7,8-Dihydroxyflavone nano-liposomes decorated by crosslinked and glycosylated lactoferrin: storage stability, antioxidant activity, in vitro release, gastrointestinal digestion and transport in Caco-2 cell monolayers
Low bioavailability and stability of 7,8-dihydroxyflavone (7,8-DHF) hinder its potential application in prevention of humans’ disease. 7,8-DHF loaded nanoliposomes decorated by original, crosslinked and glycosylated lactoferrin (LF) were fabricated. The mean particle size of spherical crosslinked an...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-02-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464619306668 |
| _version_ | 1828959490683174912 |
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| author | Yufeng Chen Guobin Xia Zhenlei Zhao Fan Xue Yanpei Gu Chun Chen Ying Zhang |
| author_facet | Yufeng Chen Guobin Xia Zhenlei Zhao Fan Xue Yanpei Gu Chun Chen Ying Zhang |
| author_sort | Yufeng Chen |
| collection | DOAJ |
| description | Low bioavailability and stability of 7,8-dihydroxyflavone (7,8-DHF) hinder its potential application in prevention of humans’ disease. 7,8-DHF loaded nanoliposomes decorated by original, crosslinked and glycosylated lactoferrin (LF) were fabricated. The mean particle size of spherical crosslinked and glycosylated LF coating liposomes were larger than uncoated liposomes with a low polydispersity index, and the former had higher encapsulation efficiency (Above 98%). Crosslinked and glycosylated LF coating liposomes had higher antioxidant activity, storage stability, slow-release ability, bioaccessibility and transepithelial transport compared to free 7,8-DHF and 7,8-DHF loaded liposome. Effective hydrogen bonding between phospholipid and 7,8-DHF, while electrostatic interaction and hydrogen bonding between LF proteins and phospholipid existed. The encapsulated 7,8-DHF was in an amorphous state rather than a crystalline form as detected by differential scanning calorimetry. Collectively, crosslinked or glycosylated LF coating liposomes are promising delivery systems for protecting and transporting 7,8-DHF or other relative bioactive components. |
| first_indexed | 2024-12-14T09:08:02Z |
| format | Article |
| id | doaj.art-5c7a771aa11942e487cabfda04ef952a |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T09:08:02Z |
| publishDate | 2020-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-5c7a771aa11942e487cabfda04ef952a2022-12-21T23:08:39ZengElsevierJournal of Functional Foods1756-46462020-02-01651037427,8-Dihydroxyflavone nano-liposomes decorated by crosslinked and glycosylated lactoferrin: storage stability, antioxidant activity, in vitro release, gastrointestinal digestion and transport in Caco-2 cell monolayersYufeng Chen0Guobin Xia1Zhenlei Zhao2Fan Xue3Yanpei Gu4Chun Chen5Ying Zhang6Department of Food Science and Nutrition, School of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing; Zhejiang Engineering Center for Food Technology and Equipment; Zhejiang University, Hangzhou 310058, ChinaChildren’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USADepartment of Food Science and Nutrition, School of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing; Zhejiang Engineering Center for Food Technology and Equipment; Zhejiang University, Hangzhou 310058, ChinaDepartment of Food Science and Nutrition, School of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing; Zhejiang Engineering Center for Food Technology and Equipment; Zhejiang University, Hangzhou 310058, ChinaDepartment of Food Science and Nutrition, School of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing; Zhejiang Engineering Center for Food Technology and Equipment; Zhejiang University, Hangzhou 310058, ChinaDepartment of Food Science and Nutrition, School of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing; Zhejiang Engineering Center for Food Technology and Equipment; Zhejiang University, Hangzhou 310058, China; Department of Pathology and Laboratory Medicine, School of Medicine, Emory University, Atlanta, GA 30322, USADepartment of Food Science and Nutrition, School of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing; Zhejiang Engineering Center for Food Technology and Equipment; Zhejiang University, Hangzhou 310058, China; Corresponding author at: College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Xihu District, Hangzhou 310058, China.Low bioavailability and stability of 7,8-dihydroxyflavone (7,8-DHF) hinder its potential application in prevention of humans’ disease. 7,8-DHF loaded nanoliposomes decorated by original, crosslinked and glycosylated lactoferrin (LF) were fabricated. The mean particle size of spherical crosslinked and glycosylated LF coating liposomes were larger than uncoated liposomes with a low polydispersity index, and the former had higher encapsulation efficiency (Above 98%). Crosslinked and glycosylated LF coating liposomes had higher antioxidant activity, storage stability, slow-release ability, bioaccessibility and transepithelial transport compared to free 7,8-DHF and 7,8-DHF loaded liposome. Effective hydrogen bonding between phospholipid and 7,8-DHF, while electrostatic interaction and hydrogen bonding between LF proteins and phospholipid existed. The encapsulated 7,8-DHF was in an amorphous state rather than a crystalline form as detected by differential scanning calorimetry. Collectively, crosslinked or glycosylated LF coating liposomes are promising delivery systems for protecting and transporting 7,8-DHF or other relative bioactive components.http://www.sciencedirect.com/science/article/pii/S17564646193066687,8-DihydroxyflavoneLactoferrinLiposomeBioaccessibilityStability |
| spellingShingle | Yufeng Chen Guobin Xia Zhenlei Zhao Fan Xue Yanpei Gu Chun Chen Ying Zhang 7,8-Dihydroxyflavone nano-liposomes decorated by crosslinked and glycosylated lactoferrin: storage stability, antioxidant activity, in vitro release, gastrointestinal digestion and transport in Caco-2 cell monolayers Journal of Functional Foods 7,8-Dihydroxyflavone Lactoferrin Liposome Bioaccessibility Stability |
| title | 7,8-Dihydroxyflavone nano-liposomes decorated by crosslinked and glycosylated lactoferrin: storage stability, antioxidant activity, in vitro release, gastrointestinal digestion and transport in Caco-2 cell monolayers |
| title_full | 7,8-Dihydroxyflavone nano-liposomes decorated by crosslinked and glycosylated lactoferrin: storage stability, antioxidant activity, in vitro release, gastrointestinal digestion and transport in Caco-2 cell monolayers |
| title_fullStr | 7,8-Dihydroxyflavone nano-liposomes decorated by crosslinked and glycosylated lactoferrin: storage stability, antioxidant activity, in vitro release, gastrointestinal digestion and transport in Caco-2 cell monolayers |
| title_full_unstemmed | 7,8-Dihydroxyflavone nano-liposomes decorated by crosslinked and glycosylated lactoferrin: storage stability, antioxidant activity, in vitro release, gastrointestinal digestion and transport in Caco-2 cell monolayers |
| title_short | 7,8-Dihydroxyflavone nano-liposomes decorated by crosslinked and glycosylated lactoferrin: storage stability, antioxidant activity, in vitro release, gastrointestinal digestion and transport in Caco-2 cell monolayers |
| title_sort | 7 8 dihydroxyflavone nano liposomes decorated by crosslinked and glycosylated lactoferrin storage stability antioxidant activity in vitro release gastrointestinal digestion and transport in caco 2 cell monolayers |
| topic | 7,8-Dihydroxyflavone Lactoferrin Liposome Bioaccessibility Stability |
| url | http://www.sciencedirect.com/science/article/pii/S1756464619306668 |
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