Shrimp miR-965 transfers tumoricidal mitochondria
Abstract Background Micro RNA of Marsupenaeus japonicas has been known to promote apoptosis of tumor cells. However, the detailed mechanisms are not well understood. Results Using tomographic microscope, which can detect the internal structure of cells, we observed breast tumor cells following treat...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-10-01
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| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12575-022-00178-8 |
| _version_ | 1811257132405751808 |
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| author | Hyueyun Kim Ji Ha Choi Chang Mo Moon Jihee Lee Kang Minna Woo Minsuk Kim |
| author_facet | Hyueyun Kim Ji Ha Choi Chang Mo Moon Jihee Lee Kang Minna Woo Minsuk Kim |
| author_sort | Hyueyun Kim |
| collection | DOAJ |
| description | Abstract Background Micro RNA of Marsupenaeus japonicas has been known to promote apoptosis of tumor cells. However, the detailed mechanisms are not well understood. Results Using tomographic microscope, which can detect the internal structure of cells, we observed breast tumor cells following treatment of the miRNA. Intriguingly, we found that mitochondria migrate to an adjacent tumor cells through a tunneling nanotube. To recapitulate this process, we engineered a microfluidic device through which mitochondria were transferred. We show that this mitochondrial transfer process released endonuclease G (Endo G) into tumor cells, which we referred to herein as unsealed mitochondria. Importantly, Endo G depleted mitochondria alone did not have tumoricidal effects. Moreover, unsealed mitochondria had synergistic apoptotic effects with subtoxic dose of doxorubicin thereby mitigating cardiotoxicity. Conclusions Together, we show that the mitochondrial transfer through microfluidics can provide potential novel strategies towards tumor cell death. |
| first_indexed | 2024-04-12T17:51:45Z |
| format | Article |
| id | doaj.art-5c7bf728830f4cc1a310929b02362c53 |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-04-12T17:51:45Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj.art-5c7bf728830f4cc1a310929b02362c532022-12-22T03:22:29ZengBMCBiological Procedures Online1480-92222022-10-0124111210.1186/s12575-022-00178-8Shrimp miR-965 transfers tumoricidal mitochondriaHyueyun Kim0Ji Ha Choi1Chang Mo Moon2Jihee Lee Kang3Minna Woo4Minsuk Kim5Department of Pharmacology, College of Medicine, Ewha Womans UniversityDepartment of Pharmacology, College of Medicine, Ewha Womans UniversityDepartment of Internal Medicine, College of Medicine, Ewha Womans UniversityDepartment of Physiology and Tissue Injury Defense Research Center, College of Medicine, Ewha Womans UniversityToronto General Hospital Research Institute and Division of Endocrinology and Metabolism, Department of Medicine, University Health Network, University of TorontoDepartment of Pharmacology, College of Medicine, Ewha Womans UniversityAbstract Background Micro RNA of Marsupenaeus japonicas has been known to promote apoptosis of tumor cells. However, the detailed mechanisms are not well understood. Results Using tomographic microscope, which can detect the internal structure of cells, we observed breast tumor cells following treatment of the miRNA. Intriguingly, we found that mitochondria migrate to an adjacent tumor cells through a tunneling nanotube. To recapitulate this process, we engineered a microfluidic device through which mitochondria were transferred. We show that this mitochondrial transfer process released endonuclease G (Endo G) into tumor cells, which we referred to herein as unsealed mitochondria. Importantly, Endo G depleted mitochondria alone did not have tumoricidal effects. Moreover, unsealed mitochondria had synergistic apoptotic effects with subtoxic dose of doxorubicin thereby mitigating cardiotoxicity. Conclusions Together, we show that the mitochondrial transfer through microfluidics can provide potential novel strategies towards tumor cell death.https://doi.org/10.1186/s12575-022-00178-8MitochondriaMicrofluidicsTunneling nanotubeBreast tumorCotton candyTomographic microscope |
| spellingShingle | Hyueyun Kim Ji Ha Choi Chang Mo Moon Jihee Lee Kang Minna Woo Minsuk Kim Shrimp miR-965 transfers tumoricidal mitochondria Biological Procedures Online Mitochondria Microfluidics Tunneling nanotube Breast tumor Cotton candy Tomographic microscope |
| title | Shrimp miR-965 transfers tumoricidal mitochondria |
| title_full | Shrimp miR-965 transfers tumoricidal mitochondria |
| title_fullStr | Shrimp miR-965 transfers tumoricidal mitochondria |
| title_full_unstemmed | Shrimp miR-965 transfers tumoricidal mitochondria |
| title_short | Shrimp miR-965 transfers tumoricidal mitochondria |
| title_sort | shrimp mir 965 transfers tumoricidal mitochondria |
| topic | Mitochondria Microfluidics Tunneling nanotube Breast tumor Cotton candy Tomographic microscope |
| url | https://doi.org/10.1186/s12575-022-00178-8 |
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