Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork
AIM: To investigate potential gene changes in trabecular meshwork (TM) induced by dexamethasone (DEX) in steroid-induced glaucoma (SIG). METHODS: The expression data of 24 cases from a public functional genomics data were sorted to identify the mechanisms of action of DEX on the TM. The relationship...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2022-08-01
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| Series: | International Journal of Ophthalmology |
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| Online Access: | http://ies.ijo.cn/en_publish/2022/8/20220803.pdf |
| _version_ | 1818017602806480896 |
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| author | Miao Wei Lu-Ming Chen Ze-Yu Huang Guo-Wei Zhang Huai-Jin Guan Min Ji |
| author_facet | Miao Wei Lu-Ming Chen Ze-Yu Huang Guo-Wei Zhang Huai-Jin Guan Min Ji |
| author_sort | Miao Wei |
| collection | DOAJ |
| description | AIM: To investigate potential gene changes in trabecular meshwork (TM) induced by dexamethasone (DEX) in steroid-induced glaucoma (SIG). METHODS: The expression data of 24 cases from a public functional genomics data were sorted to identify the mechanisms of action of DEX on the TM. The relationships of the differentially expressed genes (DEGs) were enriched using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. In addition, the hub genes were screened by the Search Tool for the Retrieval of Interacting Genes Database (STRING) and Cytoscape tools. Finally, human TM cells (HTMCs) were treated with DEX to preliminarily explore the function of hub genes. RESULTS: Totally 47 DEGs, including 21 downregulated and 26 upregulated genes were identified. The primary enriched results of the DEGs consisted of inflammatory response, extracellular matrix (ECM), negative regulation of cell proliferation, TNF signalling pathway and the regulation of tryptophan channels by inflammatory mediators. Subsequently, pro-melanin-enriched hormone (PMCH) and Bradykinin B1 receptor (BDKRB1) were screened as hub genes. It is verified in GSE37474 data set. Western blot and quantitative real-time polymerase chain reaction (qPCR) results showed that protein and RNA expression levels of BDKRB1 were significantly decreased after DEX treatment, while PMCH was not significantly changed. CONCLUSION: BDKRB1 may be a key gene involved in SIG onset, providing a suitable therapeutic target for improving the prognosis of SIG patients. |
| first_indexed | 2024-04-14T07:29:09Z |
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| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-04-14T07:29:09Z |
| publishDate | 2022-08-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-5c7cf5fc39024580ad2fa057b26a33df2022-12-22T02:05:55ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982022-08-011581240124810.18240/ijo.2022.08.0320220803Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshworkMiao Wei0Lu-Ming Chen1Ze-Yu Huang2Guo-Wei Zhang3Huai-Jin Guan4Min Ji5Min Ji. Eye Institute, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China. amyji1234@hotmail.comEye Institute, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, ChinaEye Institute, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, ChinaEye Institute, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, ChinaEye Institute, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, ChinaEye Institute, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, ChinaAIM: To investigate potential gene changes in trabecular meshwork (TM) induced by dexamethasone (DEX) in steroid-induced glaucoma (SIG). METHODS: The expression data of 24 cases from a public functional genomics data were sorted to identify the mechanisms of action of DEX on the TM. The relationships of the differentially expressed genes (DEGs) were enriched using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. In addition, the hub genes were screened by the Search Tool for the Retrieval of Interacting Genes Database (STRING) and Cytoscape tools. Finally, human TM cells (HTMCs) were treated with DEX to preliminarily explore the function of hub genes. RESULTS: Totally 47 DEGs, including 21 downregulated and 26 upregulated genes were identified. The primary enriched results of the DEGs consisted of inflammatory response, extracellular matrix (ECM), negative regulation of cell proliferation, TNF signalling pathway and the regulation of tryptophan channels by inflammatory mediators. Subsequently, pro-melanin-enriched hormone (PMCH) and Bradykinin B1 receptor (BDKRB1) were screened as hub genes. It is verified in GSE37474 data set. Western blot and quantitative real-time polymerase chain reaction (qPCR) results showed that protein and RNA expression levels of BDKRB1 were significantly decreased after DEX treatment, while PMCH was not significantly changed. CONCLUSION: BDKRB1 may be a key gene involved in SIG onset, providing a suitable therapeutic target for improving the prognosis of SIG patients.http://ies.ijo.cn/en_publish/2022/8/20220803.pdfdexamethasonetrabecular meshwork cellssteroid-induced glaucomadifferentially expressed genesprotein-protein interaction |
| spellingShingle | Miao Wei Lu-Ming Chen Ze-Yu Huang Guo-Wei Zhang Huai-Jin Guan Min Ji Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork International Journal of Ophthalmology dexamethasone trabecular meshwork cells steroid-induced glaucoma differentially expressed genes protein-protein interaction |
| title | Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork |
| title_full | Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork |
| title_fullStr | Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork |
| title_full_unstemmed | Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork |
| title_short | Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork |
| title_sort | expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork |
| topic | dexamethasone trabecular meshwork cells steroid-induced glaucoma differentially expressed genes protein-protein interaction |
| url | http://ies.ijo.cn/en_publish/2022/8/20220803.pdf |
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