[<sup>68</sup>Ga]Ga-Pentixafor and Sodium [<sup>18</sup>F]Fluoride PET Can Non-Invasively Identify and Monitor the Dynamics of Orthodontic Tooth Movement in Mouse Model
The cellular and molecular mechanisms of orthodontic tooth movement (OTM) are not yet fully understood, partly due to the lack of dynamical datasets within the same subject. Inflammation and calcification are two main processes during OTM. Given the high sensitivity and specificity of [<sup>68...
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MDPI AG
2022-09-01
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| author | Rogerio B. Craveiro Alexandru Florea Christian Niederau Sihem Brenji Fabian Kiessling Sabri E. M. Sahnoun Agnieszka Morgenroth Felix M. Mottaghy Michael Wolf |
| author_facet | Rogerio B. Craveiro Alexandru Florea Christian Niederau Sihem Brenji Fabian Kiessling Sabri E. M. Sahnoun Agnieszka Morgenroth Felix M. Mottaghy Michael Wolf |
| author_sort | Rogerio B. Craveiro |
| collection | DOAJ |
| description | The cellular and molecular mechanisms of orthodontic tooth movement (OTM) are not yet fully understood, partly due to the lack of dynamical datasets within the same subject. Inflammation and calcification are two main processes during OTM. Given the high sensitivity and specificity of [<sup>68</sup>Ga]Ga-Pentixafor and Sodium [<sup>18</sup>F]Fluoride (Na[<sup>18</sup>F]F) for inflammation and calcification, respectively, the aim of this study is to assess their ability to identify and monitor the dynamics of OTM in an established mouse model. To monitor the processes during OTM in real time, animals were scanned using a small animal PET/CT during week 1, 3, and 5 post-implantation, with [<sup>68</sup>Ga]Ga-Pentixafor and Na[<sup>18</sup>F]F. Both tracers showed an increased uptake in the region of interest compared to the control. For [<sup>68</sup>Ga]Ga-Pentixafor, an increased uptake was observed within the 5-week trial, suggesting the continuous presence of inflammatory markers. Na[<sup>18</sup>F]F showed an increased uptake during the trial, indicating an intensification of bone remodelling. Interim and end-of-experiment histological assessments visualised increased amounts of chemokine receptor CXCR4 and TRAP-positive cells in the periodontal ligament on the compression side. This approach establishes the first in vivo model for periodontal remodelling during OTM, which efficiently detects and monitors the intricate dynamics of periodontal ligament. |
| first_indexed | 2024-03-09T21:54:46Z |
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| language | English |
| last_indexed | 2024-03-09T21:54:46Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
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| series | Cells |
| spelling | doaj.art-5c7ef464e94f4f85af90d622e0558bd32023-11-23T20:00:01ZengMDPI AGCells2073-44092022-09-011119294910.3390/cells11192949[<sup>68</sup>Ga]Ga-Pentixafor and Sodium [<sup>18</sup>F]Fluoride PET Can Non-Invasively Identify and Monitor the Dynamics of Orthodontic Tooth Movement in Mouse ModelRogerio B. Craveiro0Alexandru Florea1Christian Niederau2Sihem Brenji3Fabian Kiessling4Sabri E. M. Sahnoun5Agnieszka Morgenroth6Felix M. Mottaghy7Michael Wolf8Department of Orthodontics, University Hospital RWTH Aachen, 52074 Aachen, GermanyDepartment of Nuclear Medicine, University Hospital RWTH Aachen, 52074 Aachen, GermanyDepartment of Orthodontics, University Hospital RWTH Aachen, 52074 Aachen, GermanyDepartment of Orthodontics, University Hospital RWTH Aachen, 52074 Aachen, GermanyInstitute for Experimental Molecular Imaging, University Clinic Aachen, RWTH Aachen University, 52074 Aachen, GermanyDepartment of Nuclear Medicine, University Hospital RWTH Aachen, 52074 Aachen, GermanyDepartment of Nuclear Medicine, University Hospital RWTH Aachen, 52074 Aachen, GermanyDepartment of Nuclear Medicine, University Hospital RWTH Aachen, 52074 Aachen, GermanyDepartment of Orthodontics, University Hospital RWTH Aachen, 52074 Aachen, GermanyThe cellular and molecular mechanisms of orthodontic tooth movement (OTM) are not yet fully understood, partly due to the lack of dynamical datasets within the same subject. Inflammation and calcification are two main processes during OTM. Given the high sensitivity and specificity of [<sup>68</sup>Ga]Ga-Pentixafor and Sodium [<sup>18</sup>F]Fluoride (Na[<sup>18</sup>F]F) for inflammation and calcification, respectively, the aim of this study is to assess their ability to identify and monitor the dynamics of OTM in an established mouse model. To monitor the processes during OTM in real time, animals were scanned using a small animal PET/CT during week 1, 3, and 5 post-implantation, with [<sup>68</sup>Ga]Ga-Pentixafor and Na[<sup>18</sup>F]F. Both tracers showed an increased uptake in the region of interest compared to the control. For [<sup>68</sup>Ga]Ga-Pentixafor, an increased uptake was observed within the 5-week trial, suggesting the continuous presence of inflammatory markers. Na[<sup>18</sup>F]F showed an increased uptake during the trial, indicating an intensification of bone remodelling. Interim and end-of-experiment histological assessments visualised increased amounts of chemokine receptor CXCR4 and TRAP-positive cells in the periodontal ligament on the compression side. This approach establishes the first in vivo model for periodontal remodelling during OTM, which efficiently detects and monitors the intricate dynamics of periodontal ligament.https://www.mdpi.com/2073-4409/11/19/2949calcification[<sup>68</sup>Ga]Ga-Pentixaforinflammationin vivo remodelinglong-term monitoringorthodontic tooth movement |
| spellingShingle | Rogerio B. Craveiro Alexandru Florea Christian Niederau Sihem Brenji Fabian Kiessling Sabri E. M. Sahnoun Agnieszka Morgenroth Felix M. Mottaghy Michael Wolf [<sup>68</sup>Ga]Ga-Pentixafor and Sodium [<sup>18</sup>F]Fluoride PET Can Non-Invasively Identify and Monitor the Dynamics of Orthodontic Tooth Movement in Mouse Model Cells calcification [<sup>68</sup>Ga]Ga-Pentixafor inflammation in vivo remodeling long-term monitoring orthodontic tooth movement |
| title | [<sup>68</sup>Ga]Ga-Pentixafor and Sodium [<sup>18</sup>F]Fluoride PET Can Non-Invasively Identify and Monitor the Dynamics of Orthodontic Tooth Movement in Mouse Model |
| title_full | [<sup>68</sup>Ga]Ga-Pentixafor and Sodium [<sup>18</sup>F]Fluoride PET Can Non-Invasively Identify and Monitor the Dynamics of Orthodontic Tooth Movement in Mouse Model |
| title_fullStr | [<sup>68</sup>Ga]Ga-Pentixafor and Sodium [<sup>18</sup>F]Fluoride PET Can Non-Invasively Identify and Monitor the Dynamics of Orthodontic Tooth Movement in Mouse Model |
| title_full_unstemmed | [<sup>68</sup>Ga]Ga-Pentixafor and Sodium [<sup>18</sup>F]Fluoride PET Can Non-Invasively Identify and Monitor the Dynamics of Orthodontic Tooth Movement in Mouse Model |
| title_short | [<sup>68</sup>Ga]Ga-Pentixafor and Sodium [<sup>18</sup>F]Fluoride PET Can Non-Invasively Identify and Monitor the Dynamics of Orthodontic Tooth Movement in Mouse Model |
| title_sort | sup 68 sup ga ga pentixafor and sodium sup 18 sup f fluoride pet can non invasively identify and monitor the dynamics of orthodontic tooth movement in mouse model |
| topic | calcification [<sup>68</sup>Ga]Ga-Pentixafor inflammation in vivo remodeling long-term monitoring orthodontic tooth movement |
| url | https://www.mdpi.com/2073-4409/11/19/2949 |
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