Screening and Application of Bacillus Strains Isolated from Nonrhizospheric Rice Soil for the Biocontrol of Rice Blast
Rice blast, caused by Magnaporthe oryzae, is one of the most destructive rice diseases worldwide. The aim of this study was to screen bacterial isolates to efficiently prevent the occurrence of rice blast. A total of 232 bacterial isolates were extracted from nonrhizospheric rice soil and were scree...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hanrimwon Publishing Company
2020-06-01
|
| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ppjonline.org/journal/view.html?doi=10.5423/PPJ.OA.02.2020.0028 |
| _version_ | 1829493050906247168 |
|---|---|
| author | Yuexia Sha Qingchao Zeng Shuting Sui |
| author_facet | Yuexia Sha Qingchao Zeng Shuting Sui |
| author_sort | Yuexia Sha |
| collection | DOAJ |
| description | Rice blast, caused by Magnaporthe oryzae, is one of the most destructive rice diseases worldwide. The aim of this study was to screen bacterial isolates to efficiently prevent the occurrence of rice blast. A total of 232 bacterial isolates were extracted from nonrhizospheric rice soil and were screened for antifungal activity against M. oryzae using a leaf segment assay. Strains S170 and S9 showed significant antagonistic activity against M. oryzae in vitro and in leaf disk assays, and controlled M. oryzae infection under greenhouse conditions. The results showed that strains S170 and S9 could effectively control rice leaf blast and panicle neck blast after five spray treatments in field. This suggested that the bacterial strains S170 and S9 were valuable and promising for the biocontrol of rice disease caused by M. oryzae. Based on 16S rDNA, and gyrA and gyrB gene sequence analyses, S170 and S9 were identified as Bacillus amyloliquefaciens and B. pumilus, respectively. The research also demonstrated that B. amyloliquefaciens S170 and B. pumilus S9 could colonize rice plants to prevent pathogenic infection and evidently suppressed plant disease caused by 11 other plant pathogenic fungi. This is the first study to demonstrate that B. amyloliquefaciens and B. pumilus isolated from nonrhizospheric rice soil are capable of recolonizing internal rice stem tissues. |
| first_indexed | 2024-12-16T06:26:10Z |
| format | Article |
| id | doaj.art-5c834f397e324257b150d057f4a80b74 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-12-16T06:26:10Z |
| publishDate | 2020-06-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-5c834f397e324257b150d057f4a80b742022-12-21T22:41:00ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542020-06-0136323124310.5423/PPJ.OA.02.2020.002810.5423PPJ.OA.02.2020.0028Screening and Application of Bacillus Strains Isolated from Nonrhizospheric Rice Soil for the Biocontrol of Rice BlastYuexia ShaQingchao ZengShuting SuiRice blast, caused by Magnaporthe oryzae, is one of the most destructive rice diseases worldwide. The aim of this study was to screen bacterial isolates to efficiently prevent the occurrence of rice blast. A total of 232 bacterial isolates were extracted from nonrhizospheric rice soil and were screened for antifungal activity against M. oryzae using a leaf segment assay. Strains S170 and S9 showed significant antagonistic activity against M. oryzae in vitro and in leaf disk assays, and controlled M. oryzae infection under greenhouse conditions. The results showed that strains S170 and S9 could effectively control rice leaf blast and panicle neck blast after five spray treatments in field. This suggested that the bacterial strains S170 and S9 were valuable and promising for the biocontrol of rice disease caused by M. oryzae. Based on 16S rDNA, and gyrA and gyrB gene sequence analyses, S170 and S9 were identified as Bacillus amyloliquefaciens and B. pumilus, respectively. The research also demonstrated that B. amyloliquefaciens S170 and B. pumilus S9 could colonize rice plants to prevent pathogenic infection and evidently suppressed plant disease caused by 11 other plant pathogenic fungi. This is the first study to demonstrate that B. amyloliquefaciens and B. pumilus isolated from nonrhizospheric rice soil are capable of recolonizing internal rice stem tissues.http://www.ppjonline.org/journal/view.html?doi=10.5423/PPJ.OA.02.2020.0028bacillusbiocontrolnonrhizospheric rice soilrice blastscreening |
| spellingShingle | Yuexia Sha Qingchao Zeng Shuting Sui Screening and Application of Bacillus Strains Isolated from Nonrhizospheric Rice Soil for the Biocontrol of Rice Blast The Plant Pathology Journal bacillus biocontrol nonrhizospheric rice soil rice blast screening |
| title | Screening and Application of Bacillus Strains Isolated from Nonrhizospheric Rice Soil for the Biocontrol of Rice Blast |
| title_full | Screening and Application of Bacillus Strains Isolated from Nonrhizospheric Rice Soil for the Biocontrol of Rice Blast |
| title_fullStr | Screening and Application of Bacillus Strains Isolated from Nonrhizospheric Rice Soil for the Biocontrol of Rice Blast |
| title_full_unstemmed | Screening and Application of Bacillus Strains Isolated from Nonrhizospheric Rice Soil for the Biocontrol of Rice Blast |
| title_short | Screening and Application of Bacillus Strains Isolated from Nonrhizospheric Rice Soil for the Biocontrol of Rice Blast |
| title_sort | screening and application of bacillus strains isolated from nonrhizospheric rice soil for the biocontrol of rice blast |
| topic | bacillus biocontrol nonrhizospheric rice soil rice blast screening |
| url | http://www.ppjonline.org/journal/view.html?doi=10.5423/PPJ.OA.02.2020.0028 |
| work_keys_str_mv | AT yuexiasha screeningandapplicationofbacillusstrainsisolatedfromnonrhizosphericricesoilforthebiocontrolofriceblast AT qingchaozeng screeningandapplicationofbacillusstrainsisolatedfromnonrhizosphericricesoilforthebiocontrolofriceblast AT shutingsui screeningandapplicationofbacillusstrainsisolatedfromnonrhizosphericricesoilforthebiocontrolofriceblast |