Improved osteoblast function on titanium implant surfaces coated with nanocomposite Apatite–Wollastonite–Chitosan– an experimental in-vitro study
Abstract Background There is a continuous research in the area of biomimetic coatings on the titanium (Ti) implant surfaces for improved survival and long-term successful outcomes in the field of dentistry and orthopedics. In-vitro approaches are ideal systems for studying cell-material interactions...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2022-02-01
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| Series: | Journal of Materials Science: Materials in Medicine |
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| Online Access: | https://doi.org/10.1007/s10856-022-06651-w |
| _version_ | 1818501258856628224 |
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| author | Shayanti Mukherjee Smriti Sharma Vivek Soni Amruta Joshi Amit Gaikwad Jayesh Bellare Jyoti Kode |
| author_facet | Shayanti Mukherjee Smriti Sharma Vivek Soni Amruta Joshi Amit Gaikwad Jayesh Bellare Jyoti Kode |
| author_sort | Shayanti Mukherjee |
| collection | DOAJ |
| description | Abstract Background There is a continuous research in the area of biomimetic coatings on the titanium (Ti) implant surfaces for improved survival and long-term successful outcomes in the field of dentistry and orthopedics. In-vitro approaches are ideal systems for studying cell-material interactions without complexity and interference observed in in-vivo models. Purpose The present study was undertaken to evaluate the osteoblast characteristics and function on Ti substrates coated with the novel composite coating of ceramic apatite-wollastonite (AW) and polymer chitosan. Materials and methods Ti substrate coated with composite AW-Chitosan was synthesized, using electrophoretic deposition. MG-63 cells were seeded onto the coated substrates and cellular morphology and growth was assessed using Scanning Electron Microscopy (SEM) and Laser Scanning Microscopy (LSM). Osteocalcin expression of the seeded cells was assessed by FITC tagging and LSM analysis. Alizarin Red S staining and Confocal LSM (CSLM) analysis was used to study the in-vitro mineralization on the titanium samples. Results The AW-Chitosan coating on Ti samples by electrophoretic deposition exerted significant positive influence on cell proliferation, growth and mineralization as compared to uncoated titanium samples. Scanning electron microscopy and laser confocal microscopy experiments revealed that the coating was non-toxic to cells, enhanced adhesion and proliferation of MG-63 cells. Increased functional activity was observed by increased production of bone-specific protein osteocalcin and mineralized calcium through day 7 and 14. Conclusions The present study underscores that optimal inorganic-organic phase nanocomposite crack-free coating created on Ti by simple, cost-effective electrophoretic deposition technique may have osteoconductive potential and may have wide application in the field of implantology. Graphical abstract |
| first_indexed | 2024-12-10T20:53:43Z |
| format | Article |
| id | doaj.art-5c86743e1b644fc9931e0481038b46a6 |
| institution | Directory Open Access Journal |
| issn | 0957-4530 1573-4838 |
| language | English |
| last_indexed | 2024-12-10T20:53:43Z |
| publishDate | 2022-02-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj.art-5c86743e1b644fc9931e0481038b46a62022-12-22T01:34:01ZengSpringerJournal of Materials Science: Materials in Medicine0957-45301573-48382022-02-0133311110.1007/s10856-022-06651-wImproved osteoblast function on titanium implant surfaces coated with nanocomposite Apatite–Wollastonite–Chitosan– an experimental in-vitro studyShayanti Mukherjee0Smriti Sharma1Vivek Soni2Amruta Joshi3Amit Gaikwad4Jayesh Bellare5Jyoti Kode6Kode Lab, Tumor Immunology & Immunotherapy Group, Advanced Centre for Treatment, Research & Education in Cancer, Tata Memorial CentreDepartment of Chemical Engineering, School of Biosciences and Bioengineering, Indian Institute of Technology BombayDepartment of Chemical Engineering and Wadhwani Research Center for Bioengineering, IIT-BombayDepartment of Periodontics, MGM Dental College and HospitalDepartment of Prosthodontics, MGM Dental College and HospitalDepartment of Chemical Engineering and Wadhwani Research Center for Bioengineering, IIT-BombayKode Lab, Tumor Immunology & Immunotherapy Group, Advanced Centre for Treatment, Research & Education in Cancer, Tata Memorial CentreAbstract Background There is a continuous research in the area of biomimetic coatings on the titanium (Ti) implant surfaces for improved survival and long-term successful outcomes in the field of dentistry and orthopedics. In-vitro approaches are ideal systems for studying cell-material interactions without complexity and interference observed in in-vivo models. Purpose The present study was undertaken to evaluate the osteoblast characteristics and function on Ti substrates coated with the novel composite coating of ceramic apatite-wollastonite (AW) and polymer chitosan. Materials and methods Ti substrate coated with composite AW-Chitosan was synthesized, using electrophoretic deposition. MG-63 cells were seeded onto the coated substrates and cellular morphology and growth was assessed using Scanning Electron Microscopy (SEM) and Laser Scanning Microscopy (LSM). Osteocalcin expression of the seeded cells was assessed by FITC tagging and LSM analysis. Alizarin Red S staining and Confocal LSM (CSLM) analysis was used to study the in-vitro mineralization on the titanium samples. Results The AW-Chitosan coating on Ti samples by electrophoretic deposition exerted significant positive influence on cell proliferation, growth and mineralization as compared to uncoated titanium samples. Scanning electron microscopy and laser confocal microscopy experiments revealed that the coating was non-toxic to cells, enhanced adhesion and proliferation of MG-63 cells. Increased functional activity was observed by increased production of bone-specific protein osteocalcin and mineralized calcium through day 7 and 14. Conclusions The present study underscores that optimal inorganic-organic phase nanocomposite crack-free coating created on Ti by simple, cost-effective electrophoretic deposition technique may have osteoconductive potential and may have wide application in the field of implantology. Graphical abstracthttps://doi.org/10.1007/s10856-022-06651-wBiomimetic materialDental/Orthopedic implantOsseointegrationOsteoblastTitaniumSystematic in vitro approach |
| spellingShingle | Shayanti Mukherjee Smriti Sharma Vivek Soni Amruta Joshi Amit Gaikwad Jayesh Bellare Jyoti Kode Improved osteoblast function on titanium implant surfaces coated with nanocomposite Apatite–Wollastonite–Chitosan– an experimental in-vitro study Journal of Materials Science: Materials in Medicine Biomimetic material Dental/Orthopedic implant Osseointegration Osteoblast Titanium Systematic in vitro approach |
| title | Improved osteoblast function on titanium implant surfaces coated with nanocomposite Apatite–Wollastonite–Chitosan– an experimental in-vitro study |
| title_full | Improved osteoblast function on titanium implant surfaces coated with nanocomposite Apatite–Wollastonite–Chitosan– an experimental in-vitro study |
| title_fullStr | Improved osteoblast function on titanium implant surfaces coated with nanocomposite Apatite–Wollastonite–Chitosan– an experimental in-vitro study |
| title_full_unstemmed | Improved osteoblast function on titanium implant surfaces coated with nanocomposite Apatite–Wollastonite–Chitosan– an experimental in-vitro study |
| title_short | Improved osteoblast function on titanium implant surfaces coated with nanocomposite Apatite–Wollastonite–Chitosan– an experimental in-vitro study |
| title_sort | improved osteoblast function on titanium implant surfaces coated with nanocomposite apatite wollastonite chitosan an experimental in vitro study |
| topic | Biomimetic material Dental/Orthopedic implant Osseointegration Osteoblast Titanium Systematic in vitro approach |
| url | https://doi.org/10.1007/s10856-022-06651-w |
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