Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): hints of cultivation stress-induced genomic changes
Bartonelloses are neglected emerging infectious diseases caused by facultatively intracellular bacteria transmitted between vertebrate hosts by various arthropod vectors. The highest diversity of Bartonella species has been identified in rodents. Within this study we focused on the edible dormouse (...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-11-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1289671/full |
| _version_ | 1797614204790767616 |
|---|---|
| author | Oldřich Bartoš Běla Klimešová Karolina Volfová Martin Chmel Martin Chmel Jiří Dresler Petr Pajer Hana Kabíčková Peter Adamík Peter Adamík David Modrý David Modrý David Modrý Alena Myslivcová Fučíková Jan Votýpka Jan Votýpka |
| author_facet | Oldřich Bartoš Běla Klimešová Karolina Volfová Martin Chmel Martin Chmel Jiří Dresler Petr Pajer Hana Kabíčková Peter Adamík Peter Adamík David Modrý David Modrý David Modrý Alena Myslivcová Fučíková Jan Votýpka Jan Votýpka |
| author_sort | Oldřich Bartoš |
| collection | DOAJ |
| description | Bartonelloses are neglected emerging infectious diseases caused by facultatively intracellular bacteria transmitted between vertebrate hosts by various arthropod vectors. The highest diversity of Bartonella species has been identified in rodents. Within this study we focused on the edible dormouse (Glis glis), a rodent with unique life-history traits that often enters households and whose possible role in the epidemiology of Bartonella infections had been previously unknown. We identified and cultivated two distinct Bartonella sub(species) significantly diverging from previously described species, which were characterized using growth characteristics, biochemical tests, and various molecular techniques including also proteomics. Two novel (sub)species were described: Bartonella grahamii subsp. shimonis subsp. nov. and Bartonella gliris sp. nov. We sequenced two individual strains per each described (sub)species. During exploratory genomic analyses comparing two genotypes ultimately belonging to the same species, both factually and most importantly even spatiotemporally, we noticed unexpectedly significant structural variation between them. We found that most of the detected structural variants could be explained either by prophage excision or integration. Based on a detailed study of one such event, we argue that prophage deletion represents the most probable explanation of the observed phenomena. Moreover, in one strain of Bartonella grahamii subsp. shimonis subsp. nov. we identified a deletion related to Bartonella Adhesin A, a major pathogenicity factor that modulates bacteria-host interactions. Altogether, our results suggest that even a limited number of passages induced sufficient selective pressure to promote significant changes at the level of the genome. |
| first_indexed | 2024-03-11T07:06:30Z |
| format | Article |
| id | doaj.art-5c8788f5b7d44e33a50af652aeaa4973 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-11T07:06:30Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5c8788f5b7d44e33a50af652aeaa49732023-11-17T08:56:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-11-011410.3389/fmicb.2023.12896711289671Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): hints of cultivation stress-induced genomic changesOldřich Bartoš0Běla Klimešová1Karolina Volfová2Martin Chmel3Martin Chmel4Jiří Dresler5Petr Pajer6Hana Kabíčková7Peter Adamík8Peter Adamík9David Modrý10David Modrý11David Modrý12Alena Myslivcová Fučíková13Jan Votýpka14Jan Votýpka15Military Health Institute, Military Medical Agency, Prague, CzechiaDepartment of Parasitology, Faculty of Science, Charles University, Prague, CzechiaDepartment of Parasitology, Faculty of Science, Charles University, Prague, CzechiaMilitary Health Institute, Military Medical Agency, Prague, CzechiaDepartment of Infectious Diseases, First Faculty of Medicine, Charles University and Military University Hospital Prague, Prague, CzechiaMilitary Health Institute, Military Medical Agency, Prague, CzechiaMilitary Health Institute, Military Medical Agency, Prague, CzechiaMilitary Health Institute, Military Medical Agency, Prague, CzechiaDepartment of Zoology, Faculty of Science, Palacký University, Olomouc, CzechiaMuseum of Natural History, Olomouc, CzechiaInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, CzechiaDepartment of Botany and Zoology, Faculty of Science, Masaryk University, Brno, CzechiaDepartment of Veterinary Sciences/CINeZ, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, CzechiaDepartment of Biology, University of Hradec Králové, Hradec Králové, CzechiaDepartment of Parasitology, Faculty of Science, Charles University, Prague, CzechiaInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, CzechiaBartonelloses are neglected emerging infectious diseases caused by facultatively intracellular bacteria transmitted between vertebrate hosts by various arthropod vectors. The highest diversity of Bartonella species has been identified in rodents. Within this study we focused on the edible dormouse (Glis glis), a rodent with unique life-history traits that often enters households and whose possible role in the epidemiology of Bartonella infections had been previously unknown. We identified and cultivated two distinct Bartonella sub(species) significantly diverging from previously described species, which were characterized using growth characteristics, biochemical tests, and various molecular techniques including also proteomics. Two novel (sub)species were described: Bartonella grahamii subsp. shimonis subsp. nov. and Bartonella gliris sp. nov. We sequenced two individual strains per each described (sub)species. During exploratory genomic analyses comparing two genotypes ultimately belonging to the same species, both factually and most importantly even spatiotemporally, we noticed unexpectedly significant structural variation between them. We found that most of the detected structural variants could be explained either by prophage excision or integration. Based on a detailed study of one such event, we argue that prophage deletion represents the most probable explanation of the observed phenomena. Moreover, in one strain of Bartonella grahamii subsp. shimonis subsp. nov. we identified a deletion related to Bartonella Adhesin A, a major pathogenicity factor that modulates bacteria-host interactions. Altogether, our results suggest that even a limited number of passages induced sufficient selective pressure to promote significant changes at the level of the genome.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1289671/fullBartonella glirisBartonella grahamii subsp. shimonisBartonella Adhesin Acultivation-related genomic changesgene deletion |
| spellingShingle | Oldřich Bartoš Běla Klimešová Karolina Volfová Martin Chmel Martin Chmel Jiří Dresler Petr Pajer Hana Kabíčková Peter Adamík Peter Adamík David Modrý David Modrý David Modrý Alena Myslivcová Fučíková Jan Votýpka Jan Votýpka Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): hints of cultivation stress-induced genomic changes Frontiers in Microbiology Bartonella gliris Bartonella grahamii subsp. shimonis Bartonella Adhesin A cultivation-related genomic changes gene deletion |
| title | Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): hints of cultivation stress-induced genomic changes |
| title_full | Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): hints of cultivation stress-induced genomic changes |
| title_fullStr | Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): hints of cultivation stress-induced genomic changes |
| title_full_unstemmed | Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): hints of cultivation stress-induced genomic changes |
| title_short | Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): hints of cultivation stress-induced genomic changes |
| title_sort | two novel bartonella sub species isolated from edible dormice glis glis hints of cultivation stress induced genomic changes |
| topic | Bartonella gliris Bartonella grahamii subsp. shimonis Bartonella Adhesin A cultivation-related genomic changes gene deletion |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1289671/full |
| work_keys_str_mv | AT oldrichbartos twonovelbartonellasubspeciesisolatedfromedibledormiceglisglishintsofcultivationstressinducedgenomicchanges AT belaklimesova twonovelbartonellasubspeciesisolatedfromedibledormiceglisglishintsofcultivationstressinducedgenomicchanges AT karolinavolfova twonovelbartonellasubspeciesisolatedfromedibledormiceglisglishintsofcultivationstressinducedgenomicchanges AT martinchmel twonovelbartonellasubspeciesisolatedfromedibledormiceglisglishintsofcultivationstressinducedgenomicchanges AT martinchmel twonovelbartonellasubspeciesisolatedfromedibledormiceglisglishintsofcultivationstressinducedgenomicchanges AT jiridresler twonovelbartonellasubspeciesisolatedfromedibledormiceglisglishintsofcultivationstressinducedgenomicchanges AT petrpajer twonovelbartonellasubspeciesisolatedfromedibledormiceglisglishintsofcultivationstressinducedgenomicchanges AT hanakabickova twonovelbartonellasubspeciesisolatedfromedibledormiceglisglishintsofcultivationstressinducedgenomicchanges AT peteradamik twonovelbartonellasubspeciesisolatedfromedibledormiceglisglishintsofcultivationstressinducedgenomicchanges AT peteradamik twonovelbartonellasubspeciesisolatedfromedibledormiceglisglishintsofcultivationstressinducedgenomicchanges AT davidmodry twonovelbartonellasubspeciesisolatedfromedibledormiceglisglishintsofcultivationstressinducedgenomicchanges AT davidmodry twonovelbartonellasubspeciesisolatedfromedibledormiceglisglishintsofcultivationstressinducedgenomicchanges AT davidmodry twonovelbartonellasubspeciesisolatedfromedibledormiceglisglishintsofcultivationstressinducedgenomicchanges AT alenamyslivcovafucikova twonovelbartonellasubspeciesisolatedfromedibledormiceglisglishintsofcultivationstressinducedgenomicchanges AT janvotypka twonovelbartonellasubspeciesisolatedfromedibledormiceglisglishintsofcultivationstressinducedgenomicchanges AT janvotypka twonovelbartonellasubspeciesisolatedfromedibledormiceglisglishintsofcultivationstressinducedgenomicchanges |