Distinct lung microbiota associate with HIV-associated chronic lung disease in children
Abstract Chronic lung disease (CLD) is a common co-morbidity for HIV-positive children and adolescents on antiretroviral therapy (ART) in sub-Saharan Africa. In this population, distinct airway microbiota may differentially confer risk of CLD. In a cross-sectional study of 202 HIV-infected children...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-73085-1 |
| _version_ | 1818460298567221248 |
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| author | Sudha Bhadriraju Douglas W. Fadrosh Meera K. Shenoy Din L. Lin Kole V. Lynch Kathryn McCauley Rashida A. Ferrand Edith D. Majonga Grace McHugh Laurence Huang Susan V. Lynch John Z. Metcalfe |
| author_facet | Sudha Bhadriraju Douglas W. Fadrosh Meera K. Shenoy Din L. Lin Kole V. Lynch Kathryn McCauley Rashida A. Ferrand Edith D. Majonga Grace McHugh Laurence Huang Susan V. Lynch John Z. Metcalfe |
| author_sort | Sudha Bhadriraju |
| collection | DOAJ |
| description | Abstract Chronic lung disease (CLD) is a common co-morbidity for HIV-positive children and adolescents on antiretroviral therapy (ART) in sub-Saharan Africa. In this population, distinct airway microbiota may differentially confer risk of CLD. In a cross-sectional study of 202 HIV-infected children aged 6–16 years in Harare, Zimbabwe, we determined the association of sputum microbiota composition (using 16S ribosomal RNA V4 gene region sequencing) with CLD defined using clinical, spirometric, or radiographic criteria. Forty-two percent of children were determined to have CLD according to our definition. Dirichlet multinomial mixtures identified four compositionally distinct sputum microbiota structures. Patients whose sputum microbiota was dominated by Haemophilus, Moraxella or Neisseria (HMN) were at 1.5 times higher risk of CLD than those with Streptococcus or Prevotella (SP)-dominated microbiota (RR = 1.48, p = 0.035). Cell-free products of HMN sputum microbiota induced features of epithelial disruption and inflammatory gene expression in vitro, indicating enhanced pathogenic potential of these CLD-associated microbiota. Thus, HIV-positive children harbor distinct sputum microbiota, with those dominated by Haemophilus, Moraxella or Neisseria associated with enhanced pathogenesis in vitro and clinical CLD. |
| first_indexed | 2024-12-14T23:28:02Z |
| format | Article |
| id | doaj.art-5c889e806c114c2e94ed9a46227d7dc5 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T23:28:02Z |
| publishDate | 2020-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5c889e806c114c2e94ed9a46227d7dc52022-12-21T22:43:45ZengNature PortfolioScientific Reports2045-23222020-09-011011910.1038/s41598-020-73085-1Distinct lung microbiota associate with HIV-associated chronic lung disease in childrenSudha Bhadriraju0Douglas W. Fadrosh1Meera K. Shenoy2Din L. Lin3Kole V. Lynch4Kathryn McCauley5Rashida A. Ferrand6Edith D. Majonga7Grace McHugh8Laurence Huang9Susan V. Lynch10John Z. Metcalfe11Division of Pulmonary and Critical Care Medicine, San Francisco General Hospital and Trauma Center, University of California San FranciscoDivision of Gastroenterology, Department of Medicine, University of CaliforniaDivision of Gastroenterology, Department of Medicine, University of CaliforniaDivision of Gastroenterology, Department of Medicine, University of CaliforniaDivision of Gastroenterology, Department of Medicine, University of CaliforniaDivision of Gastroenterology, Department of Medicine, University of CaliforniaBiomedical Research and Training InstituteBiomedical Research and Training InstituteBiomedical Research and Training InstituteDivision of Pulmonary and Critical Care Medicine, San Francisco General Hospital and Trauma Center, University of California San FranciscoDivision of Gastroenterology, Department of Medicine, University of CaliforniaDivision of Pulmonary and Critical Care Medicine, San Francisco General Hospital and Trauma Center, University of California San FranciscoAbstract Chronic lung disease (CLD) is a common co-morbidity for HIV-positive children and adolescents on antiretroviral therapy (ART) in sub-Saharan Africa. In this population, distinct airway microbiota may differentially confer risk of CLD. In a cross-sectional study of 202 HIV-infected children aged 6–16 years in Harare, Zimbabwe, we determined the association of sputum microbiota composition (using 16S ribosomal RNA V4 gene region sequencing) with CLD defined using clinical, spirometric, or radiographic criteria. Forty-two percent of children were determined to have CLD according to our definition. Dirichlet multinomial mixtures identified four compositionally distinct sputum microbiota structures. Patients whose sputum microbiota was dominated by Haemophilus, Moraxella or Neisseria (HMN) were at 1.5 times higher risk of CLD than those with Streptococcus or Prevotella (SP)-dominated microbiota (RR = 1.48, p = 0.035). Cell-free products of HMN sputum microbiota induced features of epithelial disruption and inflammatory gene expression in vitro, indicating enhanced pathogenic potential of these CLD-associated microbiota. Thus, HIV-positive children harbor distinct sputum microbiota, with those dominated by Haemophilus, Moraxella or Neisseria associated with enhanced pathogenesis in vitro and clinical CLD.https://doi.org/10.1038/s41598-020-73085-1 |
| spellingShingle | Sudha Bhadriraju Douglas W. Fadrosh Meera K. Shenoy Din L. Lin Kole V. Lynch Kathryn McCauley Rashida A. Ferrand Edith D. Majonga Grace McHugh Laurence Huang Susan V. Lynch John Z. Metcalfe Distinct lung microbiota associate with HIV-associated chronic lung disease in children Scientific Reports |
| title | Distinct lung microbiota associate with HIV-associated chronic lung disease in children |
| title_full | Distinct lung microbiota associate with HIV-associated chronic lung disease in children |
| title_fullStr | Distinct lung microbiota associate with HIV-associated chronic lung disease in children |
| title_full_unstemmed | Distinct lung microbiota associate with HIV-associated chronic lung disease in children |
| title_short | Distinct lung microbiota associate with HIV-associated chronic lung disease in children |
| title_sort | distinct lung microbiota associate with hiv associated chronic lung disease in children |
| url | https://doi.org/10.1038/s41598-020-73085-1 |
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