Persistence of a Yeast-Based (<i>Hanseniaspora uvarum</i>) Attract-and-Kill Formulation against <i>Drosophila suzukii</i> on Grape Leaves
The production of phagostimulant and attractive volatile organic compounds (VOCs) by yeasts can be exploited to improve the efficacy of attract-and-kill formulations against the spotted wing drosophila (SWD). This study evaluated the persistence over one week of a yeast-based formulation under green...
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MDPI AG
2020-11-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/11/810 |
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| author | Flavia Bianchi Urban Spitaler Irene Castellan Carlo S. Cossu Timothy Brigadoi Claire Duménil Sergio Angeli Peter Robatscher Rudi F. Vogel Silvia Schmidt Daniela Eisenstecken |
| author_facet | Flavia Bianchi Urban Spitaler Irene Castellan Carlo S. Cossu Timothy Brigadoi Claire Duménil Sergio Angeli Peter Robatscher Rudi F. Vogel Silvia Schmidt Daniela Eisenstecken |
| author_sort | Flavia Bianchi |
| collection | DOAJ |
| description | The production of phagostimulant and attractive volatile organic compounds (VOCs) by yeasts can be exploited to improve the efficacy of attract-and-kill formulations against the spotted wing drosophila (SWD). This study evaluated the persistence over one week of a yeast-based formulation under greenhouse conditions. Potted grape plants were treated with: (i) potato dextrose broth (PDB), (ii) PDB containing spinosad (PDB + S), and (iii) <i>H. uvarum</i> fermentation broth grown on PDB containing spinosad (H. u. + S). Laboratory trials were performed to determine the survival and the oviposition rate of SWD after exposure to treated leaves. Ion-exchange chromatography was performed to measure carbohydrates, sugar alcohols, and organic acids on leaf surfaces, while amino acids were assessed through liquid chromatography–mass-spectrometry. Additionally, the VOCs released by plants treated with <i>H.uvarum</i> were collected via closed-loop-stripping analysis and compared to those emitted by untreated leaves. A higher mortality was observed for adult SWDs in contact with <i>H. uvarum</i> containing spinosad compared to PDB containing spinosad. Generally, a decrease in the amounts of non-volatile compounds was observed over time, though numerous nutrients were still present one week after treatment. The application of the yeast-based formulation induced the emission of VOCs by the treated leaves. The concentration of 2-phenylethanol, one of the main VOCs emitted by yeasts, decreased over time. These findings describe the presence of potential phagostimulants and compounds attractive to SWD in a yeast-based attract-and-kill formulation and demonstrate the efficacy of the formulation over one week. |
| first_indexed | 2024-03-10T14:45:48Z |
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| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T14:45:48Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-5c8b9554de2c496f9b7255dfe551d80a2023-11-20T21:21:43ZengMDPI AGInsects2075-44502020-11-01111181010.3390/insects11110810Persistence of a Yeast-Based (<i>Hanseniaspora uvarum</i>) Attract-and-Kill Formulation against <i>Drosophila suzukii</i> on Grape LeavesFlavia Bianchi0Urban Spitaler1Irene Castellan2Carlo S. Cossu3Timothy Brigadoi4Claire Duménil5Sergio Angeli6Peter Robatscher7Rudi F. Vogel8Silvia Schmidt9Daniela Eisenstecken10Laboratory for Flavours and Metabolites, Institute for Agricultural Chemistry and Food Quality, Laimburg Research Centre, Laimburg 6, 39040 Auer (Ora), South Tyrol, ItalyEntomology Group, Institute for Plant Health, Laimburg Research Centre, Laimburg 6, 39040 Auer (Ora), South Tyrol, ItalyFaculty of Science and Technology, Free University of Bozen-Bolzano, 39100 Bolzano, ItalyEntomology Group, Institute for Plant Health, Laimburg Research Centre, Laimburg 6, 39040 Auer (Ora), South Tyrol, ItalyLaboratory for Flavours and Metabolites, Institute for Agricultural Chemistry and Food Quality, Laimburg Research Centre, Laimburg 6, 39040 Auer (Ora), South Tyrol, ItalyFaculty of Science and Technology, Free University of Bozen-Bolzano, 39100 Bolzano, ItalyFaculty of Science and Technology, Free University of Bozen-Bolzano, 39100 Bolzano, ItalyLaboratory for Flavours and Metabolites, Institute for Agricultural Chemistry and Food Quality, Laimburg Research Centre, Laimburg 6, 39040 Auer (Ora), South Tyrol, ItalyChair of Technical Microbiology, TUM School of Life Sciences, Technical University of Munich, Gregor-Mendel-Straße 4, 85354 Freising, GermanyEntomology Group, Institute for Plant Health, Laimburg Research Centre, Laimburg 6, 39040 Auer (Ora), South Tyrol, ItalyLaboratory for Flavours and Metabolites, Institute for Agricultural Chemistry and Food Quality, Laimburg Research Centre, Laimburg 6, 39040 Auer (Ora), South Tyrol, ItalyThe production of phagostimulant and attractive volatile organic compounds (VOCs) by yeasts can be exploited to improve the efficacy of attract-and-kill formulations against the spotted wing drosophila (SWD). This study evaluated the persistence over one week of a yeast-based formulation under greenhouse conditions. Potted grape plants were treated with: (i) potato dextrose broth (PDB), (ii) PDB containing spinosad (PDB + S), and (iii) <i>H. uvarum</i> fermentation broth grown on PDB containing spinosad (H. u. + S). Laboratory trials were performed to determine the survival and the oviposition rate of SWD after exposure to treated leaves. Ion-exchange chromatography was performed to measure carbohydrates, sugar alcohols, and organic acids on leaf surfaces, while amino acids were assessed through liquid chromatography–mass-spectrometry. Additionally, the VOCs released by plants treated with <i>H.uvarum</i> were collected via closed-loop-stripping analysis and compared to those emitted by untreated leaves. A higher mortality was observed for adult SWDs in contact with <i>H. uvarum</i> containing spinosad compared to PDB containing spinosad. Generally, a decrease in the amounts of non-volatile compounds was observed over time, though numerous nutrients were still present one week after treatment. The application of the yeast-based formulation induced the emission of VOCs by the treated leaves. The concentration of 2-phenylethanol, one of the main VOCs emitted by yeasts, decreased over time. These findings describe the presence of potential phagostimulants and compounds attractive to SWD in a yeast-based attract-and-kill formulation and demonstrate the efficacy of the formulation over one week.https://www.mdpi.com/2075-4450/11/11/810spotted wing drosophilapest controlspinosadmetabolitesVOCs |
| spellingShingle | Flavia Bianchi Urban Spitaler Irene Castellan Carlo S. Cossu Timothy Brigadoi Claire Duménil Sergio Angeli Peter Robatscher Rudi F. Vogel Silvia Schmidt Daniela Eisenstecken Persistence of a Yeast-Based (<i>Hanseniaspora uvarum</i>) Attract-and-Kill Formulation against <i>Drosophila suzukii</i> on Grape Leaves Insects spotted wing drosophila pest control spinosad metabolites VOCs |
| title | Persistence of a Yeast-Based (<i>Hanseniaspora uvarum</i>) Attract-and-Kill Formulation against <i>Drosophila suzukii</i> on Grape Leaves |
| title_full | Persistence of a Yeast-Based (<i>Hanseniaspora uvarum</i>) Attract-and-Kill Formulation against <i>Drosophila suzukii</i> on Grape Leaves |
| title_fullStr | Persistence of a Yeast-Based (<i>Hanseniaspora uvarum</i>) Attract-and-Kill Formulation against <i>Drosophila suzukii</i> on Grape Leaves |
| title_full_unstemmed | Persistence of a Yeast-Based (<i>Hanseniaspora uvarum</i>) Attract-and-Kill Formulation against <i>Drosophila suzukii</i> on Grape Leaves |
| title_short | Persistence of a Yeast-Based (<i>Hanseniaspora uvarum</i>) Attract-and-Kill Formulation against <i>Drosophila suzukii</i> on Grape Leaves |
| title_sort | persistence of a yeast based i hanseniaspora uvarum i attract and kill formulation against i drosophila suzukii i on grape leaves |
| topic | spotted wing drosophila pest control spinosad metabolites VOCs |
| url | https://www.mdpi.com/2075-4450/11/11/810 |
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