Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of <i>Humulus japonicus</i> and It’s Protective Effects against Parkinson’s Disease Mouse Model
<i>Humulus japonicus</i> is an annual plant belonging to the Cannabacea family, and it has been traditionally used to treat pulmonary tuberculosis, dysentery, chronic colitis, and hypertension. We investigated the active components against Parkinson’s disease from <i>H. j...
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MDPI AG
2019-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/7/1435 |
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| author | Hee Ju Lee Basanta Dhodary Ju Yong Lee Jin-Pyo An Young-Kyoung Ryu Kyoung-Shim Kim Chul-Ho Lee Won Keun Oh |
| author_facet | Hee Ju Lee Basanta Dhodary Ju Yong Lee Jin-Pyo An Young-Kyoung Ryu Kyoung-Shim Kim Chul-Ho Lee Won Keun Oh |
| author_sort | Hee Ju Lee |
| collection | DOAJ |
| description | <i>Humulus japonicus</i> is an annual plant belonging to the Cannabacea family, and it has been traditionally used to treat pulmonary tuberculosis, dysentery, chronic colitis, and hypertension. We investigated the active components against Parkinson’s disease from <i>H. japonicus</i> fraction (HJF) using high performance liquid chromatography (HPLC) coupled with quadruple-time-of-flight mass spectroscopy (qTOF-MS) and NMR. Fourteen compounds were isolated from HJF, including one new compound, using HPLC-qTOF-MS and NMR. The major compounds of HJF were luteolin-7-<i>O</i>-glucoside and apigenin-7-<i>O</i>-glucoside, and there was approximately 12.57- and 9.68-folds increase in the contents of these flavonoids compared to those of the 70% EtOH extract. Apigenin and luteolin exhibited the strongest inhibitory effects on monoamine oxidase (MAO) B enzyme activity. In animal studies, limb-use behavior was significantly reduced by unilateral 6-OHDA lesion and ipsilateral rotations. These results indicated that oral administration of 300 mg/kg HJF resulted in the improvement of motor asymmetry and motor impairment in unilateral 6-OHDA-lesioned mice. HJF, including active components leads to an improvement of motor behavior in a Parkinson’s disease mouse model. |
| first_indexed | 2024-12-13T20:05:13Z |
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| id | doaj.art-5c8bfc860a554d4d9599ebde058d3bb4 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T20:05:13Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-5c8bfc860a554d4d9599ebde058d3bb42022-12-21T23:33:02ZengMDPI AGMolecules1420-30492019-04-01247143510.3390/molecules24071435molecules24071435Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of <i>Humulus japonicus</i> and It’s Protective Effects against Parkinson’s Disease Mouse ModelHee Ju Lee0Basanta Dhodary1Ju Yong Lee2Jin-Pyo An3Young-Kyoung Ryu4Kyoung-Shim Kim5Chul-Ho Lee6Won Keun Oh7Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 08826, KoreaKorea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 08826, KoreaKorea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 08826, KoreaKorea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 08826, KoreaLaboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, KoreaLaboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, KoreaLaboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, KoreaKorea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 08826, Korea<i>Humulus japonicus</i> is an annual plant belonging to the Cannabacea family, and it has been traditionally used to treat pulmonary tuberculosis, dysentery, chronic colitis, and hypertension. We investigated the active components against Parkinson’s disease from <i>H. japonicus</i> fraction (HJF) using high performance liquid chromatography (HPLC) coupled with quadruple-time-of-flight mass spectroscopy (qTOF-MS) and NMR. Fourteen compounds were isolated from HJF, including one new compound, using HPLC-qTOF-MS and NMR. The major compounds of HJF were luteolin-7-<i>O</i>-glucoside and apigenin-7-<i>O</i>-glucoside, and there was approximately 12.57- and 9.68-folds increase in the contents of these flavonoids compared to those of the 70% EtOH extract. Apigenin and luteolin exhibited the strongest inhibitory effects on monoamine oxidase (MAO) B enzyme activity. In animal studies, limb-use behavior was significantly reduced by unilateral 6-OHDA lesion and ipsilateral rotations. These results indicated that oral administration of 300 mg/kg HJF resulted in the improvement of motor asymmetry and motor impairment in unilateral 6-OHDA-lesioned mice. HJF, including active components leads to an improvement of motor behavior in a Parkinson’s disease mouse model.https://www.mdpi.com/1420-3049/24/7/1435<i>Humulus japonicus</i>HPLC-qTOF-MS<i>in vitro</i> MAO B assayin vivo 6-OHDA-lesioned PD animal testParkinson’s Disease |
| spellingShingle | Hee Ju Lee Basanta Dhodary Ju Yong Lee Jin-Pyo An Young-Kyoung Ryu Kyoung-Shim Kim Chul-Ho Lee Won Keun Oh Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of <i>Humulus japonicus</i> and It’s Protective Effects against Parkinson’s Disease Mouse Model Molecules <i>Humulus japonicus</i> HPLC-qTOF-MS <i>in vitro</i> MAO B assay in vivo 6-OHDA-lesioned PD animal test Parkinson’s Disease |
| title | Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of <i>Humulus japonicus</i> and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| title_full | Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of <i>Humulus japonicus</i> and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| title_fullStr | Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of <i>Humulus japonicus</i> and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| title_full_unstemmed | Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of <i>Humulus japonicus</i> and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| title_short | Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of <i>Humulus japonicus</i> and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| title_sort | dereplication of components coupled with hplc qtof ms in the active fraction of i humulus japonicus i and it s protective effects against parkinson s disease mouse model |
| topic | <i>Humulus japonicus</i> HPLC-qTOF-MS <i>in vitro</i> MAO B assay in vivo 6-OHDA-lesioned PD animal test Parkinson’s Disease |
| url | https://www.mdpi.com/1420-3049/24/7/1435 |
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