Transformation and regeneration of DNA polymerase Θ mutant rice plants
Abstract Agrobacterium T‐DNA integration into the plant genome is essential for the process of transgenesis and is widely used for genome engineering. The importance of the non‐homologous end‐joining (NHEJ) protein DNA polymerase Θ, encoded by the PolQ gene, for T‐DNA integration is controversial, w...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-09-01
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| Series: | Plant Direct |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/pld3.526 |
| _version_ | 1797629655338975232 |
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| author | Ayako Nishizawa‐Yokoi Stanton B. Gelvin |
| author_facet | Ayako Nishizawa‐Yokoi Stanton B. Gelvin |
| author_sort | Ayako Nishizawa‐Yokoi |
| collection | DOAJ |
| description | Abstract Agrobacterium T‐DNA integration into the plant genome is essential for the process of transgenesis and is widely used for genome engineering. The importance of the non‐homologous end‐joining (NHEJ) protein DNA polymerase Θ, encoded by the PolQ gene, for T‐DNA integration is controversial, with some groups claiming it is essential whereas others claim T‐DNA integration in Arabidopsis and rice polQ mutant plant tissue. Because of pleiotropic effects of PolQ loss on plant development, scientists have previously had difficulty regenerating transgenic polQ mutant plants. We describe a protocol for regenerating transgenic polQ mutant rice plants using a sequential transformation method. This protocol may be applicable to other plant species. |
| first_indexed | 2024-03-11T10:56:13Z |
| format | Article |
| id | doaj.art-5c8d839dab8a46eeb1e122cf05a7f432 |
| institution | Directory Open Access Journal |
| issn | 2475-4455 |
| language | English |
| last_indexed | 2024-03-11T10:56:13Z |
| publishDate | 2023-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj.art-5c8d839dab8a46eeb1e122cf05a7f4322023-11-13T07:55:56ZengWileyPlant Direct2475-44552023-09-0179n/an/a10.1002/pld3.526Transformation and regeneration of DNA polymerase Θ mutant rice plantsAyako Nishizawa‐Yokoi0Stanton B. Gelvin1Institute of Agrobiological Sciences National Agriculture and Food Research Organization Tsukuba JapanDepartment of Biological Sciences Purdue University West Lafayette Indiana USAAbstract Agrobacterium T‐DNA integration into the plant genome is essential for the process of transgenesis and is widely used for genome engineering. The importance of the non‐homologous end‐joining (NHEJ) protein DNA polymerase Θ, encoded by the PolQ gene, for T‐DNA integration is controversial, with some groups claiming it is essential whereas others claim T‐DNA integration in Arabidopsis and rice polQ mutant plant tissue. Because of pleiotropic effects of PolQ loss on plant development, scientists have previously had difficulty regenerating transgenic polQ mutant plants. We describe a protocol for regenerating transgenic polQ mutant rice plants using a sequential transformation method. This protocol may be applicable to other plant species.https://doi.org/10.1002/pld3.526Agrobacterium‐mediated plant transformationDNA polymerase thetaplant regenerationrice |
| spellingShingle | Ayako Nishizawa‐Yokoi Stanton B. Gelvin Transformation and regeneration of DNA polymerase Θ mutant rice plants Plant Direct Agrobacterium‐mediated plant transformation DNA polymerase theta plant regeneration rice |
| title | Transformation and regeneration of DNA polymerase Θ mutant rice plants |
| title_full | Transformation and regeneration of DNA polymerase Θ mutant rice plants |
| title_fullStr | Transformation and regeneration of DNA polymerase Θ mutant rice plants |
| title_full_unstemmed | Transformation and regeneration of DNA polymerase Θ mutant rice plants |
| title_short | Transformation and regeneration of DNA polymerase Θ mutant rice plants |
| title_sort | transformation and regeneration of dna polymerase θ mutant rice plants |
| topic | Agrobacterium‐mediated plant transformation DNA polymerase theta plant regeneration rice |
| url | https://doi.org/10.1002/pld3.526 |
| work_keys_str_mv | AT ayakonishizawayokoi transformationandregenerationofdnapolymerasethmutantriceplants AT stantonbgelvin transformationandregenerationofdnapolymerasethmutantriceplants |