Microbial Community Composition in Take-All Suppressive Soils
Gaeumannomyces graminis var. tritici (Ggt) is the main soilborne factor that affects wheat production around the world. Recently we reported the occurrence of six suppressive soils in monoculture areas from indigenous “Mapuche” communities, and evidenced that the suppression relied on the biotic com...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-09-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02198/full |
| _version_ | 1818302627509698560 |
|---|---|
| author | Paola Durán Paola Durán Gonzalo Tortella Sharon Viscardi Sharon Viscardi Patricio Javier Barra Victor J. Carrión María de la Luz Mora María José Pozo |
| author_facet | Paola Durán Paola Durán Gonzalo Tortella Sharon Viscardi Sharon Viscardi Patricio Javier Barra Victor J. Carrión María de la Luz Mora María José Pozo |
| author_sort | Paola Durán |
| collection | DOAJ |
| description | Gaeumannomyces graminis var. tritici (Ggt) is the main soilborne factor that affects wheat production around the world. Recently we reported the occurrence of six suppressive soils in monoculture areas from indigenous “Mapuche” communities, and evidenced that the suppression relied on the biotic component of those soils. Here, we compare the rhizosphere and endosphere microbial community structure (total bacteria, actinomycetes, total fungi, and ascomycetes) of wheat plants grown in suppressive and conducive soils. Our results suggested that Ggt suppression could be mediated mostly by bacterial endophytes, rather than rhizosphere microorganisms, since the community structure was similar in all suppressive soils as compared with conducive. Interestingly, we found that despite the lower incidence of take-all disease in suppressive soils, the Ggt concentration in roots was not significantly reduced in all suppressive soils compared to those growing in conducive soil. Therefore, the disease suppression is not always related to a reduction of the pathogen biomass. Furthermore, we isolated endophytic bacteria from wheat roots growing in suppressive soils. Among them we identified Serratia spp. and Enterobacter spp. able to inhibit Ggt growth in vitro. Since the disease, but not always pathogen amount, was reduced in the suppressive soils, we propose that take all disease suppressiveness is not only related to direct antagonism to the pathogen. |
| first_indexed | 2024-12-13T05:41:55Z |
| format | Article |
| id | doaj.art-5c9043bc05424227bb17f94d8d3467b7 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T05:41:55Z |
| publishDate | 2018-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5c9043bc05424227bb17f94d8d3467b72022-12-21T23:57:45ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-09-01910.3389/fmicb.2018.02198385189Microbial Community Composition in Take-All Suppressive SoilsPaola Durán0Paola Durán1Gonzalo Tortella2Sharon Viscardi3Sharon Viscardi4Patricio Javier Barra5Victor J. Carrión6María de la Luz Mora7María José Pozo8Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco, ChileBiocontrol Research Laboratory, Universidad de La Frontera, Temuco, ChileScientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco, ChileBiocontrol Research Laboratory, Universidad de La Frontera, Temuco, ChileDepartamento de Procesos Diagnósticos y Evaluación, Facultad de Ciencias de la Salud, Universidad Católica de Temuco, Temuco, ChileScientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco, ChileDepartment of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, NetherlandsScientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco, ChileDepartment of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (CSIC), Granada, SpainGaeumannomyces graminis var. tritici (Ggt) is the main soilborne factor that affects wheat production around the world. Recently we reported the occurrence of six suppressive soils in monoculture areas from indigenous “Mapuche” communities, and evidenced that the suppression relied on the biotic component of those soils. Here, we compare the rhizosphere and endosphere microbial community structure (total bacteria, actinomycetes, total fungi, and ascomycetes) of wheat plants grown in suppressive and conducive soils. Our results suggested that Ggt suppression could be mediated mostly by bacterial endophytes, rather than rhizosphere microorganisms, since the community structure was similar in all suppressive soils as compared with conducive. Interestingly, we found that despite the lower incidence of take-all disease in suppressive soils, the Ggt concentration in roots was not significantly reduced in all suppressive soils compared to those growing in conducive soil. Therefore, the disease suppression is not always related to a reduction of the pathogen biomass. Furthermore, we isolated endophytic bacteria from wheat roots growing in suppressive soils. Among them we identified Serratia spp. and Enterobacter spp. able to inhibit Ggt growth in vitro. Since the disease, but not always pathogen amount, was reduced in the suppressive soils, we propose that take all disease suppressiveness is not only related to direct antagonism to the pathogen.https://www.frontiersin.org/article/10.3389/fmicb.2018.02198/fullGaeumannomyces graminismicrobial diversitysuppressive soilstake-allreal time PCR |
| spellingShingle | Paola Durán Paola Durán Gonzalo Tortella Sharon Viscardi Sharon Viscardi Patricio Javier Barra Victor J. Carrión María de la Luz Mora María José Pozo Microbial Community Composition in Take-All Suppressive Soils Frontiers in Microbiology Gaeumannomyces graminis microbial diversity suppressive soils take-all real time PCR |
| title | Microbial Community Composition in Take-All Suppressive Soils |
| title_full | Microbial Community Composition in Take-All Suppressive Soils |
| title_fullStr | Microbial Community Composition in Take-All Suppressive Soils |
| title_full_unstemmed | Microbial Community Composition in Take-All Suppressive Soils |
| title_short | Microbial Community Composition in Take-All Suppressive Soils |
| title_sort | microbial community composition in take all suppressive soils |
| topic | Gaeumannomyces graminis microbial diversity suppressive soils take-all real time PCR |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02198/full |
| work_keys_str_mv | AT paoladuran microbialcommunitycompositionintakeallsuppressivesoils AT paoladuran microbialcommunitycompositionintakeallsuppressivesoils AT gonzalotortella microbialcommunitycompositionintakeallsuppressivesoils AT sharonviscardi microbialcommunitycompositionintakeallsuppressivesoils AT sharonviscardi microbialcommunitycompositionintakeallsuppressivesoils AT patriciojavierbarra microbialcommunitycompositionintakeallsuppressivesoils AT victorjcarrion microbialcommunitycompositionintakeallsuppressivesoils AT mariadelaluzmora microbialcommunitycompositionintakeallsuppressivesoils AT mariajosepozo microbialcommunitycompositionintakeallsuppressivesoils |