Spent Yeast Waste Streams as a Sustainable Source of Bioactive Peptides for Skin Applications
Spent yeast waste streams are a byproduct obtained from fermentation process and have been shown to be a rich secondary source of bioactive compounds such as phenolic compounds and peptides. The latter are of particular interest for skin care and cosmetics as they have been shown to be safe and hypo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/2253 |
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| author | Eduardo M. Costa Ana Sofia Oliveira Sara Silva Alessandra B. Ribeiro Carla F. Pereira Carlos Ferreira Francisca Casanova Joana O. Pereira Ricardo Freixo Manuela E. Pintado Ana Paula Carvalho Óscar L. Ramos |
| author_facet | Eduardo M. Costa Ana Sofia Oliveira Sara Silva Alessandra B. Ribeiro Carla F. Pereira Carlos Ferreira Francisca Casanova Joana O. Pereira Ricardo Freixo Manuela E. Pintado Ana Paula Carvalho Óscar L. Ramos |
| author_sort | Eduardo M. Costa |
| collection | DOAJ |
| description | Spent yeast waste streams are a byproduct obtained from fermentation process and have been shown to be a rich secondary source of bioactive compounds such as phenolic compounds and peptides. The latter are of particular interest for skin care and cosmetics as they have been shown to be safe and hypoallergenic while simultaneously being able to exert various effects upon the epidermis modulating immune response and targeting skin metabolites, such as collagen production. As the potential of spent yeast’s peptides has been mainly explored for food-related applications, this work sought to understand if peptide fractions previously extracted from fermentation engineered spent yeast (<i>Saccharomyces cerevisiae</i>) waste streams possess biological potential for skin-related applications. To that end, cytotoxic effects on HaCat and HDFa cells and whether they were capable of exerting a positive effect upon the production of skin metabolites relevant for skin health, such as collagen, hyaluronic acid, fibronectin and elastin, were evaluated. The results showed that the peptide fractions assayed were not cytotoxic up to the highest concentration tested (500 µg/mL) for both cell lines tested. Furthermore, all peptide fractions showed a capacity to modulate the various target metabolites production with an overall positive effect being observed for the four fractions over the six selected targets (pro-collagen IαI, hyaluronic acid, fibronectin, cytokeratin-14, elastin, and aquaporin-9). Concerning the evaluated fractions, the overall best performance (Gpep > 1 kDa) was of an average promotion of 41.25% over the six metabolites and two cell lines assessed at a concentration of 100 µg/mL. These results showed that the peptide fractions assayed in this work have potential for future applications in skin-related products at relatively low concentrations, thus providing an alternative solution for one of the fermentation industry’s waste streams and creating a novel and highly valuable bioactive ingredient with encompassing activity to be applied in future skin care formulations. |
| first_indexed | 2024-03-11T09:41:48Z |
| format | Article |
| id | doaj.art-5c932c5d80ad4f23856f3df61a9f0f18 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:41:48Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5c932c5d80ad4f23856f3df61a9f0f182023-11-16T16:54:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01243225310.3390/ijms24032253Spent Yeast Waste Streams as a Sustainable Source of Bioactive Peptides for Skin ApplicationsEduardo M. Costa0Ana Sofia Oliveira1Sara Silva2Alessandra B. Ribeiro3Carla F. Pereira4Carlos Ferreira5Francisca Casanova6Joana O. Pereira7Ricardo Freixo8Manuela E. Pintado9Ana Paula Carvalho10Óscar L. Ramos11Escola Superior de Biotecnologia, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalEscola Superior de Biotecnologia, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalEscola Superior de Biotecnologia, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalEscola Superior de Biotecnologia, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalEscola Superior de Biotecnologia, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalEscola Superior de Biotecnologia, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalEscola Superior de Biotecnologia, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalEscola Superior de Biotecnologia, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalEscola Superior de Biotecnologia, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalEscola Superior de Biotecnologia, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalEscola Superior de Biotecnologia, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalEscola Superior de Biotecnologia, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalSpent yeast waste streams are a byproduct obtained from fermentation process and have been shown to be a rich secondary source of bioactive compounds such as phenolic compounds and peptides. The latter are of particular interest for skin care and cosmetics as they have been shown to be safe and hypoallergenic while simultaneously being able to exert various effects upon the epidermis modulating immune response and targeting skin metabolites, such as collagen production. As the potential of spent yeast’s peptides has been mainly explored for food-related applications, this work sought to understand if peptide fractions previously extracted from fermentation engineered spent yeast (<i>Saccharomyces cerevisiae</i>) waste streams possess biological potential for skin-related applications. To that end, cytotoxic effects on HaCat and HDFa cells and whether they were capable of exerting a positive effect upon the production of skin metabolites relevant for skin health, such as collagen, hyaluronic acid, fibronectin and elastin, were evaluated. The results showed that the peptide fractions assayed were not cytotoxic up to the highest concentration tested (500 µg/mL) for both cell lines tested. Furthermore, all peptide fractions showed a capacity to modulate the various target metabolites production with an overall positive effect being observed for the four fractions over the six selected targets (pro-collagen IαI, hyaluronic acid, fibronectin, cytokeratin-14, elastin, and aquaporin-9). Concerning the evaluated fractions, the overall best performance (Gpep > 1 kDa) was of an average promotion of 41.25% over the six metabolites and two cell lines assessed at a concentration of 100 µg/mL. These results showed that the peptide fractions assayed in this work have potential for future applications in skin-related products at relatively low concentrations, thus providing an alternative solution for one of the fermentation industry’s waste streams and creating a novel and highly valuable bioactive ingredient with encompassing activity to be applied in future skin care formulations.https://www.mdpi.com/1422-0067/24/3/2253spent yeastskin applicationcollagen IαIhyaluronic acidwaste stream valorization |
| spellingShingle | Eduardo M. Costa Ana Sofia Oliveira Sara Silva Alessandra B. Ribeiro Carla F. Pereira Carlos Ferreira Francisca Casanova Joana O. Pereira Ricardo Freixo Manuela E. Pintado Ana Paula Carvalho Óscar L. Ramos Spent Yeast Waste Streams as a Sustainable Source of Bioactive Peptides for Skin Applications International Journal of Molecular Sciences spent yeast skin application collagen IαI hyaluronic acid waste stream valorization |
| title | Spent Yeast Waste Streams as a Sustainable Source of Bioactive Peptides for Skin Applications |
| title_full | Spent Yeast Waste Streams as a Sustainable Source of Bioactive Peptides for Skin Applications |
| title_fullStr | Spent Yeast Waste Streams as a Sustainable Source of Bioactive Peptides for Skin Applications |
| title_full_unstemmed | Spent Yeast Waste Streams as a Sustainable Source of Bioactive Peptides for Skin Applications |
| title_short | Spent Yeast Waste Streams as a Sustainable Source of Bioactive Peptides for Skin Applications |
| title_sort | spent yeast waste streams as a sustainable source of bioactive peptides for skin applications |
| topic | spent yeast skin application collagen IαI hyaluronic acid waste stream valorization |
| url | https://www.mdpi.com/1422-0067/24/3/2253 |
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