Emergence of Carbapenem- and Tigecycline-Resistant Proteus cibarius of Animal Origin
The emergence of tet(X) and carbapenemase genes in Enterobacterales pose significant challenges to the treatment of infectious diseases. Convergence of these two categories of genes in an individual pathogen would deteriorate the antimicrobial resistance (AMR) crisis furthermore. Here, tigecycline-r...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.01940/full |
| _version_ | 1818849355079090176 |
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| author | Yan Li Qian Wang Kai Peng Yuan Liu Yuan Liu Yuan Liu Ruichao Li Ruichao Li Ruichao Li Zhiqiang Wang Zhiqiang Wang |
| author_facet | Yan Li Qian Wang Kai Peng Yuan Liu Yuan Liu Yuan Liu Ruichao Li Ruichao Li Ruichao Li Zhiqiang Wang Zhiqiang Wang |
| author_sort | Yan Li |
| collection | DOAJ |
| description | The emergence of tet(X) and carbapenemase genes in Enterobacterales pose significant challenges to the treatment of infectious diseases. Convergence of these two categories of genes in an individual pathogen would deteriorate the antimicrobial resistance (AMR) crisis furthermore. Here, tigecycline-resistant Enterobacterales strains were isolated and detected with carbapenemase genes, characterized by antimicrobial susceptibility testing, PCR, conjugation assay, whole genome sequencing, and bioinformatics analysis. Three tigecycline-resistant isolates consisting of one plasmid-mediated tet(X4)-bearing Escherichia fergusonii and two chromosomal tet(X6)-bearing Proteus cibarius were recovered from chicken feces. The tet(X4) was located on a conjugative IncX1 plasmid pHNCF11W-tetX4 encoding the identical structure as reported tet(X4)-bearing IncX1 plasmids in Escherichia coli. Among two P. cibarius strains, tet(X6) was located on two similar chromosomal MDR regions with genetic contexts IS26-aac(3)-IVa-aph(4)-Ia-ISEc59-tnpA-tet(X6)-orf-orf-ISCR2-virD2-floR-ISCR2-glmM-sul2 and IS26-aac(3)-IVa-aph(4)-Ia-ISEc59-tnpA-tet(X6)-orf-orf-ISCR2-glmM-sul2. Apart from tet(X6), P. cibarius HNCF44W harbored a novel transposon Tn6450b positive for blaNDM–1 on a conjugative plasmid. This study probed the genomic basis of three tet(X)-bearing, tigecycline-resistant strains, one of which coharbored blaNDM–1 and tet(X6), and identified P. cibarius as the important reservoir of tet(X6) variants. Emergence of P. cibarius encoding both blaNDM–1 and tet(X6) reveals a potential public health risk. |
| first_indexed | 2024-12-19T06:31:55Z |
| format | Article |
| id | doaj.art-5c94c40c7fec4a0eacf2277b180446e1 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T06:31:55Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5c94c40c7fec4a0eacf2277b180446e12022-12-21T20:32:21ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-08-011110.3389/fmicb.2020.01940570813Emergence of Carbapenem- and Tigecycline-Resistant Proteus cibarius of Animal OriginYan Li0Qian Wang1Kai Peng2Yuan Liu3Yuan Liu4Yuan Liu5Ruichao Li6Ruichao Li7Ruichao Li8Zhiqiang Wang9Zhiqiang Wang10College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaInstitute of Comparative Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaInstitute of Comparative Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaThe emergence of tet(X) and carbapenemase genes in Enterobacterales pose significant challenges to the treatment of infectious diseases. Convergence of these two categories of genes in an individual pathogen would deteriorate the antimicrobial resistance (AMR) crisis furthermore. Here, tigecycline-resistant Enterobacterales strains were isolated and detected with carbapenemase genes, characterized by antimicrobial susceptibility testing, PCR, conjugation assay, whole genome sequencing, and bioinformatics analysis. Three tigecycline-resistant isolates consisting of one plasmid-mediated tet(X4)-bearing Escherichia fergusonii and two chromosomal tet(X6)-bearing Proteus cibarius were recovered from chicken feces. The tet(X4) was located on a conjugative IncX1 plasmid pHNCF11W-tetX4 encoding the identical structure as reported tet(X4)-bearing IncX1 plasmids in Escherichia coli. Among two P. cibarius strains, tet(X6) was located on two similar chromosomal MDR regions with genetic contexts IS26-aac(3)-IVa-aph(4)-Ia-ISEc59-tnpA-tet(X6)-orf-orf-ISCR2-virD2-floR-ISCR2-glmM-sul2 and IS26-aac(3)-IVa-aph(4)-Ia-ISEc59-tnpA-tet(X6)-orf-orf-ISCR2-glmM-sul2. Apart from tet(X6), P. cibarius HNCF44W harbored a novel transposon Tn6450b positive for blaNDM–1 on a conjugative plasmid. This study probed the genomic basis of three tet(X)-bearing, tigecycline-resistant strains, one of which coharbored blaNDM–1 and tet(X6), and identified P. cibarius as the important reservoir of tet(X6) variants. Emergence of P. cibarius encoding both blaNDM–1 and tet(X6) reveals a potential public health risk.https://www.frontiersin.org/article/10.3389/fmicb.2020.01940/fulltigecycline resistancecarbapenem resistancecoexistenceplasmidchromosome |
| spellingShingle | Yan Li Qian Wang Kai Peng Yuan Liu Yuan Liu Yuan Liu Ruichao Li Ruichao Li Ruichao Li Zhiqiang Wang Zhiqiang Wang Emergence of Carbapenem- and Tigecycline-Resistant Proteus cibarius of Animal Origin Frontiers in Microbiology tigecycline resistance carbapenem resistance coexistence plasmid chromosome |
| title | Emergence of Carbapenem- and Tigecycline-Resistant Proteus cibarius of Animal Origin |
| title_full | Emergence of Carbapenem- and Tigecycline-Resistant Proteus cibarius of Animal Origin |
| title_fullStr | Emergence of Carbapenem- and Tigecycline-Resistant Proteus cibarius of Animal Origin |
| title_full_unstemmed | Emergence of Carbapenem- and Tigecycline-Resistant Proteus cibarius of Animal Origin |
| title_short | Emergence of Carbapenem- and Tigecycline-Resistant Proteus cibarius of Animal Origin |
| title_sort | emergence of carbapenem and tigecycline resistant proteus cibarius of animal origin |
| topic | tigecycline resistance carbapenem resistance coexistence plasmid chromosome |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.01940/full |
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