Hypolipidemic effect of XH601 on hamsters of Hyperlipidemia and its potential mechanism
Abstract Background The novel compound XH601 is a synthesized derivative of formononetin. The present study was to investigate the hypolipidemia effect and potential mechanism of XH601. Methods Male Golden Syrian hamsters were induced by high-fat diet (HFD) for eight weeks and the hyperlipidemic mod...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-05-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12944-017-0472-z |
| _version_ | 1818128603302330368 |
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| author | Meng-Jie Zhao Shan-Shan Wang Yao Jiang Ying Wang Hong Shen Pei Xu Hua Xiang Hong Xiao |
| author_facet | Meng-Jie Zhao Shan-Shan Wang Yao Jiang Ying Wang Hong Shen Pei Xu Hua Xiang Hong Xiao |
| author_sort | Meng-Jie Zhao |
| collection | DOAJ |
| description | Abstract Background The novel compound XH601 is a synthesized derivative of formononetin. The present study was to investigate the hypolipidemia effect and potential mechanism of XH601. Methods Male Golden Syrian hamsters were induced by high-fat diet (HFD) for eight weeks and the hyperlipidemic model was established successfully. After XH601 treatment, serum and hepatic biochemistry parameters of hamsters were detected and the effect of XH601 on adipose tissue was also analyzed. Furthermore, 3 T3-L1 cell differentiation by Oil-Red-O staining was observed and the mRNA and protein expression of peroxisome proliferator-activated receptors (PPARs) were measured by qRT-PCR and Western-blot in mature adipocytes. Results The in vivo results suggest that XH601 significantly decreased the adipose weight and levels of serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL-C), apolipoprotein B (Apo-B), apolipoprotein E (Apo-E), while increased serum high-density lipoprotein (HDL-C). The in vitro results implied that XH601 up-regulated the mRNA and protein expression of both PPARα and PPARβ/δ in a dose-dependent manner. Conclusions The study suggests that XH601 exhibited strong ability to improve the dyslipidemia in hamsters fed with high-fat diet. The potential mechanism of XH601 was associated with the up-regulation of PPARα and PPARβ/δ mRNA and protein expression. |
| first_indexed | 2024-12-11T07:35:52Z |
| format | Article |
| id | doaj.art-5c956c5667624e408908e6325c83cbdc |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-11T07:35:52Z |
| publishDate | 2017-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-5c956c5667624e408908e6325c83cbdc2022-12-22T01:15:42ZengBMCLipids in Health and Disease1476-511X2017-05-0116111210.1186/s12944-017-0472-zHypolipidemic effect of XH601 on hamsters of Hyperlipidemia and its potential mechanismMeng-Jie Zhao0Shan-Shan Wang1Yao Jiang2Ying Wang3Hong Shen4Pei Xu5Hua Xiang6Hong Xiao7Nanjing Medical University, Affiliated Nanjing Brain HospitalNanjing Medical University, Affiliated Nanjing Brain HospitalNanjing Medical University, Affiliated Nanjing Brain HospitalNanjing Medical University, Affiliated Nanjing Brain HospitalNanjing Medical University, Affiliated Nanjing Brain HospitalDepartment of Pharmaceutical Chemistry, China Pharmaceutical UniversityDepartment of Pharmaceutical Chemistry, China Pharmaceutical UniversityNanjing Medical University, Affiliated Nanjing Brain HospitalAbstract Background The novel compound XH601 is a synthesized derivative of formononetin. The present study was to investigate the hypolipidemia effect and potential mechanism of XH601. Methods Male Golden Syrian hamsters were induced by high-fat diet (HFD) for eight weeks and the hyperlipidemic model was established successfully. After XH601 treatment, serum and hepatic biochemistry parameters of hamsters were detected and the effect of XH601 on adipose tissue was also analyzed. Furthermore, 3 T3-L1 cell differentiation by Oil-Red-O staining was observed and the mRNA and protein expression of peroxisome proliferator-activated receptors (PPARs) were measured by qRT-PCR and Western-blot in mature adipocytes. Results The in vivo results suggest that XH601 significantly decreased the adipose weight and levels of serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL-C), apolipoprotein B (Apo-B), apolipoprotein E (Apo-E), while increased serum high-density lipoprotein (HDL-C). The in vitro results implied that XH601 up-regulated the mRNA and protein expression of both PPARα and PPARβ/δ in a dose-dependent manner. Conclusions The study suggests that XH601 exhibited strong ability to improve the dyslipidemia in hamsters fed with high-fat diet. The potential mechanism of XH601 was associated with the up-regulation of PPARα and PPARβ/δ mRNA and protein expression.http://link.springer.com/article/10.1186/s12944-017-0472-zXH601HyperlipidemiaGolden Syrian hamster3 T3-L1 adipocytePPARs |
| spellingShingle | Meng-Jie Zhao Shan-Shan Wang Yao Jiang Ying Wang Hong Shen Pei Xu Hua Xiang Hong Xiao Hypolipidemic effect of XH601 on hamsters of Hyperlipidemia and its potential mechanism Lipids in Health and Disease XH601 Hyperlipidemia Golden Syrian hamster 3 T3-L1 adipocyte PPARs |
| title | Hypolipidemic effect of XH601 on hamsters of Hyperlipidemia and its potential mechanism |
| title_full | Hypolipidemic effect of XH601 on hamsters of Hyperlipidemia and its potential mechanism |
| title_fullStr | Hypolipidemic effect of XH601 on hamsters of Hyperlipidemia and its potential mechanism |
| title_full_unstemmed | Hypolipidemic effect of XH601 on hamsters of Hyperlipidemia and its potential mechanism |
| title_short | Hypolipidemic effect of XH601 on hamsters of Hyperlipidemia and its potential mechanism |
| title_sort | hypolipidemic effect of xh601 on hamsters of hyperlipidemia and its potential mechanism |
| topic | XH601 Hyperlipidemia Golden Syrian hamster 3 T3-L1 adipocyte PPARs |
| url | http://link.springer.com/article/10.1186/s12944-017-0472-z |
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