Microcarriers promote the through interface movement of mouse trophoblast stem cells by regulating stiffness
Mechanical force is crucial in the whole process of embryonic development. However, the role of trophoblast mechanics during embryo implantation has rarely been studied. In this study, we constructed a model to explore the effect of stiffness changes in mouse trophoblast stem cells (mTSCs) on implan...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-10-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X23001524 |
| _version_ | 1827279848754642944 |
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| author | Zili Gao Jia Guo Bo Gou Zhen Gu Tan Jia Sinan Ma Liyuan Jiang Wenli Liu Lixun Zhou Qi Gu |
| author_facet | Zili Gao Jia Guo Bo Gou Zhen Gu Tan Jia Sinan Ma Liyuan Jiang Wenli Liu Lixun Zhou Qi Gu |
| author_sort | Zili Gao |
| collection | DOAJ |
| description | Mechanical force is crucial in the whole process of embryonic development. However, the role of trophoblast mechanics during embryo implantation has rarely been studied. In this study, we constructed a model to explore the effect of stiffness changes in mouse trophoblast stem cells (mTSCs) on implantation: microcarrier was prepared by sodium alginate using a droplet microfluidics system, and mTSCs were attached to the microcarrier surface with laminin modifications, called T(micro). Compared with the spheroid, formed by the self-assembly of mTSCs (T(sph)), we could regulate the stiffness of the microcarrier, making the Young's modulus of mTSCs (367.70 ± 79.81 Pa) similar to that of the blastocyst trophoblast ectoderm (432.49 ± 151.90 Pa). Moreover, T(micro) contributes to improve the adhesion rate, expansion area and invasion depth of mTSCs. Further, T(micro) was highly expressed in tissue migration-related genes due to the activation of the Rho-associated coiled-coil containing protein kinase (ROCK) pathway at relatively similar modulus of trophoblast. Overall, our study explores the embryo implantation process with a new perspective, and provides theoretical support for understanding the effect of mechanics on embryo implantation. |
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| id | doaj.art-5c96fcfefe2c46988b60f272c7b06345 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:26:51Z |
| publishDate | 2023-10-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Bioactive Materials |
| spelling | doaj.art-5c96fcfefe2c46988b60f272c7b063452024-04-16T21:46:16ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2023-10-0128196205Microcarriers promote the through interface movement of mouse trophoblast stem cells by regulating stiffnessZili Gao0Jia Guo1Bo Gou2Zhen Gu3Tan Jia4Sinan Ma5Liyuan Jiang6Wenli Liu7Lixun Zhou8Qi Gu9State Key Laboratory of Membrane Biology, The State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, PR China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR ChinaState Key Laboratory of Membrane Biology, The State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, PR China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR ChinaState Key Laboratory of Membrane Biology, The State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, PR China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR ChinaDepartment of Chemistry and Biological Engineering, University of Science and Technology, Beijing, 100083, PR ChinaState Key Laboratory of Membrane Biology, The State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, PR China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR ChinaState Key Laboratory of Membrane Biology, The State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, PR China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, PR China; School of Life Sciences, Northeast Agricultural University, Harbin, 150030, PR ChinaState Key Laboratory of Membrane Biology, The State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, PR China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, PR China; School of Life Sciences, Northeast Agricultural University, Harbin, 150030, PR ChinaState Key Laboratory of Membrane Biology, The State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, PR China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, PR ChinaState Key Laboratory of Membrane Biology, The State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, PR China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, PR ChinaState Key Laboratory of Membrane Biology, The State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, PR China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China; Corresponding author. State Key Laboratory of Membrane Biology, The State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, PR China.Mechanical force is crucial in the whole process of embryonic development. However, the role of trophoblast mechanics during embryo implantation has rarely been studied. In this study, we constructed a model to explore the effect of stiffness changes in mouse trophoblast stem cells (mTSCs) on implantation: microcarrier was prepared by sodium alginate using a droplet microfluidics system, and mTSCs were attached to the microcarrier surface with laminin modifications, called T(micro). Compared with the spheroid, formed by the self-assembly of mTSCs (T(sph)), we could regulate the stiffness of the microcarrier, making the Young's modulus of mTSCs (367.70 ± 79.81 Pa) similar to that of the blastocyst trophoblast ectoderm (432.49 ± 151.90 Pa). Moreover, T(micro) contributes to improve the adhesion rate, expansion area and invasion depth of mTSCs. Further, T(micro) was highly expressed in tissue migration-related genes due to the activation of the Rho-associated coiled-coil containing protein kinase (ROCK) pathway at relatively similar modulus of trophoblast. Overall, our study explores the embryo implantation process with a new perspective, and provides theoretical support for understanding the effect of mechanics on embryo implantation.http://www.sciencedirect.com/science/article/pii/S2452199X23001524BiomechanicsMicrocarriersEmbryo implantationCell migration |
| spellingShingle | Zili Gao Jia Guo Bo Gou Zhen Gu Tan Jia Sinan Ma Liyuan Jiang Wenli Liu Lixun Zhou Qi Gu Microcarriers promote the through interface movement of mouse trophoblast stem cells by regulating stiffness Bioactive Materials Biomechanics Microcarriers Embryo implantation Cell migration |
| title | Microcarriers promote the through interface movement of mouse trophoblast stem cells by regulating stiffness |
| title_full | Microcarriers promote the through interface movement of mouse trophoblast stem cells by regulating stiffness |
| title_fullStr | Microcarriers promote the through interface movement of mouse trophoblast stem cells by regulating stiffness |
| title_full_unstemmed | Microcarriers promote the through interface movement of mouse trophoblast stem cells by regulating stiffness |
| title_short | Microcarriers promote the through interface movement of mouse trophoblast stem cells by regulating stiffness |
| title_sort | microcarriers promote the through interface movement of mouse trophoblast stem cells by regulating stiffness |
| topic | Biomechanics Microcarriers Embryo implantation Cell migration |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X23001524 |
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