Isoprocarb causes neurotoxicity of zebrafish embryos through oxidative stress-induced apoptosis
Isoprocarb is a widely used carbamate insecticide in agriculture and aquaculture. Overuse of isoprocarb always leaves toxic residues in soil and water, however, the potential ecotoxicity of isoprocarb to organisms is still confusing. In this study, zebrafish embryo was used as a model to evaluate th...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322007102 |
| _version_ | 1811214926651326464 |
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| author | Shanghong Wang Xue Han Tingting Yu Yulong Liu Hongying Zhang Huiling Mao Chengyu Hu Xiaowen Xu |
| author_facet | Shanghong Wang Xue Han Tingting Yu Yulong Liu Hongying Zhang Huiling Mao Chengyu Hu Xiaowen Xu |
| author_sort | Shanghong Wang |
| collection | DOAJ |
| description | Isoprocarb is a widely used carbamate insecticide in agriculture and aquaculture. Overuse of isoprocarb always leaves toxic residues in soil and water, however, the potential ecotoxicity of isoprocarb to organisms is still confusing. In this study, zebrafish embryo was used as a model to evaluate the toxicity of isoprocarb. Zebrafish embryos (96 hpf) were separately exposed at different concentrations of isoprocarb. The mortality rate, hatchability rate, average heart beat of the zebrafish embryo were separately calculated. Our results suggested that exposure to isoprocarb induced developmental toxicity in zebrafish embryos. HE staining showed that exposure to isoprocarb caused developmental defect in the hindbrain of zebrafish embryos. As expected, the behavioral analysis also showed that the motor ability of zebrafish embryos were significantly inhibited following exposure to isoprocarb. In terms of mechanism, The expressions of genes involved in neurodevelopment signaling pathways, such as foxo3a, gfap, syn2a, elavl3 and sox19b, were inhibited in zebrafish embryos after exposure to isoprocarb. The acetylcholinesterase (AChE) activity was also reduced in isoprocarb-treated zebrafish embryos. Moreover, oxidative stress was induced by increasing the reactive oxygen species (ROS) level and decreasing the activity of antioxidant enzyme (SOD) after exposure to isoprocarb. Expectedly, acridine orange (AO) staining and the detection of some apoptosis-related genes revealed that oxidative stress resulted in apoptosis. In short, the expressions of genes associated with the neurodevelopmental signaling pathway are inhibited, and oxidative stress is also induced in zebrafish embryos after exposure to isoprocarb, which may be the molecular basics of isoprocarb-induced neurotoxicity in zebrafish embryos. |
| first_indexed | 2024-04-12T06:12:45Z |
| format | Article |
| id | doaj.art-5c9a5d3d668b4091be7e8c62ab42591e |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-12T06:12:45Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-5c9a5d3d668b4091be7e8c62ab42591e2022-12-22T03:44:37ZengElsevierEcotoxicology and Environmental Safety0147-65132022-09-01242113870Isoprocarb causes neurotoxicity of zebrafish embryos through oxidative stress-induced apoptosisShanghong Wang0Xue Han1Tingting Yu2Yulong Liu3Hongying Zhang4Huiling Mao5Chengyu Hu6Xiaowen Xu7School of Life Science, Nanchang University, Nanchang 330031, Jiangxi, ChinaSchool of Life Science, Nanchang University, Nanchang 330031, Jiangxi, ChinaSchool of Life Science, Nanchang University, Nanchang 330031, Jiangxi, ChinaSchool of Life Science, Nanchang University, Nanchang 330031, Jiangxi, ChinaSchool of Life Science, Nanchang University, Nanchang 330031, Jiangxi, ChinaSchool of Life Science, Nanchang University, Nanchang 330031, Jiangxi, ChinaSchool of Life Science, Nanchang University, Nanchang 330031, Jiangxi, ChinaSchool of Life Science, Nanchang University, Nanchang 330031, Jiangxi, China; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi, China; Jiangxi Provincial Key Laboratory of Interdisciplinary Science, Nanchang University, Nanchang 330031, Jiangxi, China; Correspondence to: Department of Bioscience, School of Life Science, Nanchang University, Nanchang 330031, China.Isoprocarb is a widely used carbamate insecticide in agriculture and aquaculture. Overuse of isoprocarb always leaves toxic residues in soil and water, however, the potential ecotoxicity of isoprocarb to organisms is still confusing. In this study, zebrafish embryo was used as a model to evaluate the toxicity of isoprocarb. Zebrafish embryos (96 hpf) were separately exposed at different concentrations of isoprocarb. The mortality rate, hatchability rate, average heart beat of the zebrafish embryo were separately calculated. Our results suggested that exposure to isoprocarb induced developmental toxicity in zebrafish embryos. HE staining showed that exposure to isoprocarb caused developmental defect in the hindbrain of zebrafish embryos. As expected, the behavioral analysis also showed that the motor ability of zebrafish embryos were significantly inhibited following exposure to isoprocarb. In terms of mechanism, The expressions of genes involved in neurodevelopment signaling pathways, such as foxo3a, gfap, syn2a, elavl3 and sox19b, were inhibited in zebrafish embryos after exposure to isoprocarb. The acetylcholinesterase (AChE) activity was also reduced in isoprocarb-treated zebrafish embryos. Moreover, oxidative stress was induced by increasing the reactive oxygen species (ROS) level and decreasing the activity of antioxidant enzyme (SOD) after exposure to isoprocarb. Expectedly, acridine orange (AO) staining and the detection of some apoptosis-related genes revealed that oxidative stress resulted in apoptosis. In short, the expressions of genes associated with the neurodevelopmental signaling pathway are inhibited, and oxidative stress is also induced in zebrafish embryos after exposure to isoprocarb, which may be the molecular basics of isoprocarb-induced neurotoxicity in zebrafish embryos.http://www.sciencedirect.com/science/article/pii/S0147651322007102Carbamate insecticideZebrafish embryosNeurodevelopmentOxidative stressApoptosis |
| spellingShingle | Shanghong Wang Xue Han Tingting Yu Yulong Liu Hongying Zhang Huiling Mao Chengyu Hu Xiaowen Xu Isoprocarb causes neurotoxicity of zebrafish embryos through oxidative stress-induced apoptosis Ecotoxicology and Environmental Safety Carbamate insecticide Zebrafish embryos Neurodevelopment Oxidative stress Apoptosis |
| title | Isoprocarb causes neurotoxicity of zebrafish embryos through oxidative stress-induced apoptosis |
| title_full | Isoprocarb causes neurotoxicity of zebrafish embryos through oxidative stress-induced apoptosis |
| title_fullStr | Isoprocarb causes neurotoxicity of zebrafish embryos through oxidative stress-induced apoptosis |
| title_full_unstemmed | Isoprocarb causes neurotoxicity of zebrafish embryos through oxidative stress-induced apoptosis |
| title_short | Isoprocarb causes neurotoxicity of zebrafish embryos through oxidative stress-induced apoptosis |
| title_sort | isoprocarb causes neurotoxicity of zebrafish embryos through oxidative stress induced apoptosis |
| topic | Carbamate insecticide Zebrafish embryos Neurodevelopment Oxidative stress Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322007102 |
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