Comparative Transcriptome Analysis Reveals <i>OsBGs</i> and <i>OsGSLs</i> Influence Sugar Transport through Callose Metabolism under Heat Stress in Rice
Heat or high temperature stress have caused huge damage to many crops and have become the largest threat in terms of the future. Although a huge amount of research has been conducted to explore the mechanisms of heat tolerance and many achievements were accomplished, the mechanism by which how heat...
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/4/3175 |
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| author | Ruiwei Luan Jingyang Liu Longxing Tao Guanfu Fu Caixia Zhang |
| author_facet | Ruiwei Luan Jingyang Liu Longxing Tao Guanfu Fu Caixia Zhang |
| author_sort | Ruiwei Luan |
| collection | DOAJ |
| description | Heat or high temperature stress have caused huge damage to many crops and have become the largest threat in terms of the future. Although a huge amount of research has been conducted to explore the mechanisms of heat tolerance and many achievements were accomplished, the mechanism by which how heat stress (HS) influences the yield is still unclear. In this study, RNA-seq analysis indicated that nine 1,3-β-glucanases (<i>BGs</i>) belonging to the carbohydrate metabolic pathway were expressed differently during heat treatment. Therefore, we identified the BGs and glucan-synthase-likes (<i>GSLs</i>) in three rice ecotypes and processed the analyses of gene gain and loss, phylogenetic relationship, duplication, and syntenic relationship. We found the possibility of an environmental adaption based on <i>BGs</i> and <i>GSLs</i> during evolution. Submicrostructure and dry matter distribution analysis confirmed that HS might block the endoplasmic sugar transport pathway by increasing callose synthesis, which may lead to decreased yield and quality in rice production. This study provides a new clue regarding rice yield and quality under HS and provides guidance to rice cultivation and heat tolerance breeding. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T08:43:56Z |
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| spelling | doaj.art-5ca7fd0d2a674b0da28289731aba85f02023-11-16T20:56:18ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244317510.3390/ijms24043175Comparative Transcriptome Analysis Reveals <i>OsBGs</i> and <i>OsGSLs</i> Influence Sugar Transport through Callose Metabolism under Heat Stress in RiceRuiwei Luan0Jingyang Liu1Longxing Tao2Guanfu Fu3Caixia Zhang4Key Laboratory of Crop Biology of China, Shandong Agricultural University, Taian 271018, ChinaKey Laboratory of Crop Biology of China, Shandong Agricultural University, Taian 271018, ChinaNational Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaNational Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaKey Laboratory of Crop Biology of China, Shandong Agricultural University, Taian 271018, ChinaHeat or high temperature stress have caused huge damage to many crops and have become the largest threat in terms of the future. Although a huge amount of research has been conducted to explore the mechanisms of heat tolerance and many achievements were accomplished, the mechanism by which how heat stress (HS) influences the yield is still unclear. In this study, RNA-seq analysis indicated that nine 1,3-β-glucanases (<i>BGs</i>) belonging to the carbohydrate metabolic pathway were expressed differently during heat treatment. Therefore, we identified the BGs and glucan-synthase-likes (<i>GSLs</i>) in three rice ecotypes and processed the analyses of gene gain and loss, phylogenetic relationship, duplication, and syntenic relationship. We found the possibility of an environmental adaption based on <i>BGs</i> and <i>GSLs</i> during evolution. Submicrostructure and dry matter distribution analysis confirmed that HS might block the endoplasmic sugar transport pathway by increasing callose synthesis, which may lead to decreased yield and quality in rice production. This study provides a new clue regarding rice yield and quality under HS and provides guidance to rice cultivation and heat tolerance breeding.https://www.mdpi.com/1422-0067/24/4/3175riceheat stress<i>BGs</i> and <i>GSLs</i>callose metabolism |
| spellingShingle | Ruiwei Luan Jingyang Liu Longxing Tao Guanfu Fu Caixia Zhang Comparative Transcriptome Analysis Reveals <i>OsBGs</i> and <i>OsGSLs</i> Influence Sugar Transport through Callose Metabolism under Heat Stress in Rice International Journal of Molecular Sciences rice heat stress <i>BGs</i> and <i>GSLs</i> callose metabolism |
| title | Comparative Transcriptome Analysis Reveals <i>OsBGs</i> and <i>OsGSLs</i> Influence Sugar Transport through Callose Metabolism under Heat Stress in Rice |
| title_full | Comparative Transcriptome Analysis Reveals <i>OsBGs</i> and <i>OsGSLs</i> Influence Sugar Transport through Callose Metabolism under Heat Stress in Rice |
| title_fullStr | Comparative Transcriptome Analysis Reveals <i>OsBGs</i> and <i>OsGSLs</i> Influence Sugar Transport through Callose Metabolism under Heat Stress in Rice |
| title_full_unstemmed | Comparative Transcriptome Analysis Reveals <i>OsBGs</i> and <i>OsGSLs</i> Influence Sugar Transport through Callose Metabolism under Heat Stress in Rice |
| title_short | Comparative Transcriptome Analysis Reveals <i>OsBGs</i> and <i>OsGSLs</i> Influence Sugar Transport through Callose Metabolism under Heat Stress in Rice |
| title_sort | comparative transcriptome analysis reveals i osbgs i and i osgsls i influence sugar transport through callose metabolism under heat stress in rice |
| topic | rice heat stress <i>BGs</i> and <i>GSLs</i> callose metabolism |
| url | https://www.mdpi.com/1422-0067/24/4/3175 |
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