Microbiome patterns across the gastrointestinal tract of the rabbitfish Siganus fuscescens
Most of our knowledge regarding the biodiversity of gut microbes comes from terrestrial organisms or marine species of economic value, with less emphasis on ecologically important species. Here we investigate the bacterial composition associated with the gut of Siganus fuscescens, a rabbitfish that...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2017-05-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/3317.pdf |
| _version_ | 1797420557722976256 |
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| author | Shaun Nielsen Jackson Wilkes Walburn Adriana Vergés Torsten Thomas Suhelen Egan |
| author_facet | Shaun Nielsen Jackson Wilkes Walburn Adriana Vergés Torsten Thomas Suhelen Egan |
| author_sort | Shaun Nielsen |
| collection | DOAJ |
| description | Most of our knowledge regarding the biodiversity of gut microbes comes from terrestrial organisms or marine species of economic value, with less emphasis on ecologically important species. Here we investigate the bacterial composition associated with the gut of Siganus fuscescens, a rabbitfish that plays an important ecological role in coastal ecosystems by consuming seaweeds. Members of Firmicutes, Bacteroidetes and delta-Proteobacteria were among the dominant taxa across samples taken from the contents and the walls (sites) of the midgut and hindgut (location). Despite the high variability among individual fish, we observed statistically significant differences in beta-diversity between gut sites and gut locations. Some bacterial taxa low in abundance in the midgut content (e.g., Desulfovibrio) were found in greater abundances on the midgut wall and within the hindgut, suggesting that the gut may select for specific groups of environmental and/or food-associated microorganisms. In contrast, some distinct taxa present in the midgut content (e.g., Synechococcus) were noticeably reduced in the midgut wall and hindgut, and are thus likely to be representative of transient microbiota. This is the first assessment of the bacterial diversity associated with the gut of S. fuscescens and highlights the need to consider the variability across different gut locations and sites when analyzing fish gut microbiomes. |
| first_indexed | 2024-03-09T07:03:10Z |
| format | Article |
| id | doaj.art-5ca89214d3e2404792137363404409c8 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:03:10Z |
| publishDate | 2017-05-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-5ca89214d3e2404792137363404409c82023-12-03T09:46:17ZengPeerJ Inc.PeerJ2167-83592017-05-015e331710.7717/peerj.3317Microbiome patterns across the gastrointestinal tract of the rabbitfish Siganus fuscescensShaun Nielsen0Jackson Wilkes Walburn1Adriana Vergés2Torsten Thomas3Suhelen Egan4Centre for Marine Bio-Innovation and School of Biological Earth and Environmental Sciences, University of New South Wales, Sydney, AustraliaCentre for Marine Bio-Innovation and School of Biological Earth and Environmental Sciences, University of New South Wales, Sydney, AustraliaCentre for Marine Bio-Innovation and School of Biological Earth and Environmental Sciences, University of New South Wales, Sydney, AustraliaCentre for Marine Bio-Innovation and School of Biological Earth and Environmental Sciences, University of New South Wales, Sydney, AustraliaCentre for Marine Bio-Innovation and School of Biological Earth and Environmental Sciences, University of New South Wales, Sydney, AustraliaMost of our knowledge regarding the biodiversity of gut microbes comes from terrestrial organisms or marine species of economic value, with less emphasis on ecologically important species. Here we investigate the bacterial composition associated with the gut of Siganus fuscescens, a rabbitfish that plays an important ecological role in coastal ecosystems by consuming seaweeds. Members of Firmicutes, Bacteroidetes and delta-Proteobacteria were among the dominant taxa across samples taken from the contents and the walls (sites) of the midgut and hindgut (location). Despite the high variability among individual fish, we observed statistically significant differences in beta-diversity between gut sites and gut locations. Some bacterial taxa low in abundance in the midgut content (e.g., Desulfovibrio) were found in greater abundances on the midgut wall and within the hindgut, suggesting that the gut may select for specific groups of environmental and/or food-associated microorganisms. In contrast, some distinct taxa present in the midgut content (e.g., Synechococcus) were noticeably reduced in the midgut wall and hindgut, and are thus likely to be representative of transient microbiota. This is the first assessment of the bacterial diversity associated with the gut of S. fuscescens and highlights the need to consider the variability across different gut locations and sites when analyzing fish gut microbiomes.https://peerj.com/articles/3317.pdfMicrobial community analysisFish gut microbiome16S rRNA gene amplicon sequencingMarine microbiologyRabbitfish |
| spellingShingle | Shaun Nielsen Jackson Wilkes Walburn Adriana Vergés Torsten Thomas Suhelen Egan Microbiome patterns across the gastrointestinal tract of the rabbitfish Siganus fuscescens PeerJ Microbial community analysis Fish gut microbiome 16S rRNA gene amplicon sequencing Marine microbiology Rabbitfish |
| title | Microbiome patterns across the gastrointestinal tract of the rabbitfish Siganus fuscescens |
| title_full | Microbiome patterns across the gastrointestinal tract of the rabbitfish Siganus fuscescens |
| title_fullStr | Microbiome patterns across the gastrointestinal tract of the rabbitfish Siganus fuscescens |
| title_full_unstemmed | Microbiome patterns across the gastrointestinal tract of the rabbitfish Siganus fuscescens |
| title_short | Microbiome patterns across the gastrointestinal tract of the rabbitfish Siganus fuscescens |
| title_sort | microbiome patterns across the gastrointestinal tract of the rabbitfish siganus fuscescens |
| topic | Microbial community analysis Fish gut microbiome 16S rRNA gene amplicon sequencing Marine microbiology Rabbitfish |
| url | https://peerj.com/articles/3317.pdf |
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