Novel Projections to the Cerebrospinal Fluid-Contacting Nucleus From the Subcortex and Limbic System in Rat
Objective: To identify the novel projections received by the cerebrospinal fluid (CSF)-contacting nucleus from the subcortex and limbic system to understand the biological functions of the nucleus.Methods: The cholera toxin subunit B (CB), a retrograde tracer, was injected into the CSF-contacting nu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-08-01
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| Series: | Frontiers in Neuroanatomy |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnana.2020.00057/full |
| _version_ | 1818256889862946816 |
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| author | Si-Yuan Song Xiao-Meng Zhai Jia-Hao Dai Lei-Lei Lu Cheng-Jing Shan Jia Hong Jun-Li Cao Li-Cai Zhang |
| author_facet | Si-Yuan Song Xiao-Meng Zhai Jia-Hao Dai Lei-Lei Lu Cheng-Jing Shan Jia Hong Jun-Li Cao Li-Cai Zhang |
| author_sort | Si-Yuan Song |
| collection | DOAJ |
| description | Objective: To identify the novel projections received by the cerebrospinal fluid (CSF)-contacting nucleus from the subcortex and limbic system to understand the biological functions of the nucleus.Methods: The cholera toxin subunit B (CB), a retrograde tracer, was injected into the CSF-contacting nucleus in Sprague–Dawley rats. After 7–10 days, the surviving rats were perfused, and the whole brain and spinal cord were sliced for CB immunofluorescence detection. The CB-positive neurons in the subcortex and limbic system were observed under a fluorescence microscope, followed by 3D reconstructed with the imaris software.Results: CB-positive neurons were found in the basal forebrain, septum, periventricular organs, preoptic area, and amygdaloid structures. Five functional areas including 46 sub-regions sent projections to the CSF-contacting nucleus. However, the projections had different densities, ranging from sparse to moderate, to dense.Conclusions: According to the projections from the subcortex and limbic system, we hypothesize that the CSF-contacting nucleus participates in emotion, cognition, homeostasis regulation, visceral activity, pain, and addiction. In this study, we illustrate the novel projections from the subcortex and limbic system to the CSF-contacting nucleus, which underlies the diverse and complicated circuits of the nucleus in body regulations. |
| first_indexed | 2024-12-12T17:34:56Z |
| format | Article |
| id | doaj.art-5ca9a23eaa1b42dda680409d78a50922 |
| institution | Directory Open Access Journal |
| issn | 1662-5129 |
| language | English |
| last_indexed | 2024-12-12T17:34:56Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroanatomy |
| spelling | doaj.art-5ca9a23eaa1b42dda680409d78a509222022-12-22T00:17:14ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292020-08-011410.3389/fnana.2020.00057578035Novel Projections to the Cerebrospinal Fluid-Contacting Nucleus From the Subcortex and Limbic System in RatSi-Yuan SongXiao-Meng ZhaiJia-Hao DaiLei-Lei LuCheng-Jing ShanJia HongJun-Li CaoLi-Cai ZhangObjective: To identify the novel projections received by the cerebrospinal fluid (CSF)-contacting nucleus from the subcortex and limbic system to understand the biological functions of the nucleus.Methods: The cholera toxin subunit B (CB), a retrograde tracer, was injected into the CSF-contacting nucleus in Sprague–Dawley rats. After 7–10 days, the surviving rats were perfused, and the whole brain and spinal cord were sliced for CB immunofluorescence detection. The CB-positive neurons in the subcortex and limbic system were observed under a fluorescence microscope, followed by 3D reconstructed with the imaris software.Results: CB-positive neurons were found in the basal forebrain, septum, periventricular organs, preoptic area, and amygdaloid structures. Five functional areas including 46 sub-regions sent projections to the CSF-contacting nucleus. However, the projections had different densities, ranging from sparse to moderate, to dense.Conclusions: According to the projections from the subcortex and limbic system, we hypothesize that the CSF-contacting nucleus participates in emotion, cognition, homeostasis regulation, visceral activity, pain, and addiction. In this study, we illustrate the novel projections from the subcortex and limbic system to the CSF-contacting nucleus, which underlies the diverse and complicated circuits of the nucleus in body regulations.https://www.frontiersin.org/article/10.3389/fnana.2020.00057/fullCSF-contacting nucleussubcortexlimbic systemprojectionretrograde trace |
| spellingShingle | Si-Yuan Song Xiao-Meng Zhai Jia-Hao Dai Lei-Lei Lu Cheng-Jing Shan Jia Hong Jun-Li Cao Li-Cai Zhang Novel Projections to the Cerebrospinal Fluid-Contacting Nucleus From the Subcortex and Limbic System in Rat Frontiers in Neuroanatomy CSF-contacting nucleus subcortex limbic system projection retrograde trace |
| title | Novel Projections to the Cerebrospinal Fluid-Contacting Nucleus From the Subcortex and Limbic System in Rat |
| title_full | Novel Projections to the Cerebrospinal Fluid-Contacting Nucleus From the Subcortex and Limbic System in Rat |
| title_fullStr | Novel Projections to the Cerebrospinal Fluid-Contacting Nucleus From the Subcortex and Limbic System in Rat |
| title_full_unstemmed | Novel Projections to the Cerebrospinal Fluid-Contacting Nucleus From the Subcortex and Limbic System in Rat |
| title_short | Novel Projections to the Cerebrospinal Fluid-Contacting Nucleus From the Subcortex and Limbic System in Rat |
| title_sort | novel projections to the cerebrospinal fluid contacting nucleus from the subcortex and limbic system in rat |
| topic | CSF-contacting nucleus subcortex limbic system projection retrograde trace |
| url | https://www.frontiersin.org/article/10.3389/fnana.2020.00057/full |
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