Genetic variants in the TORC2 gene promoter and their association with body measurement and carcass quality traits in Qinchuan cattle.
The TORC2 gene is responsible for nutrient metabolism, gluconeogenesis, myogenesis and adipogenesis through the PI3K-Akt, AMPK, glucagon and insulin resistance signaling pathways. Sequencing of PCR amplicons explored three novel SNPs at loci g.16534694G>A, g.16535011C>T, and g.16535044A>T i...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0227254 |
| _version_ | 1818833790074617856 |
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| author | Rajwali Khan Sayed Haidar Abbas Raza Hongfang Guo Wang Xiaoyu Wu Sen Syed Muhammad Suhail Abdur Rahman Irfan Ullah Ayman Hassan Abd El-Aziz Zeinab Manzari Akil Alshawi Linsen Zan |
| author_facet | Rajwali Khan Sayed Haidar Abbas Raza Hongfang Guo Wang Xiaoyu Wu Sen Syed Muhammad Suhail Abdur Rahman Irfan Ullah Ayman Hassan Abd El-Aziz Zeinab Manzari Akil Alshawi Linsen Zan |
| author_sort | Rajwali Khan |
| collection | DOAJ |
| description | The TORC2 gene is responsible for nutrient metabolism, gluconeogenesis, myogenesis and adipogenesis through the PI3K-Akt, AMPK, glucagon and insulin resistance signaling pathways. Sequencing of PCR amplicons explored three novel SNPs at loci g.16534694G>A, g.16535011C>T, and g.16535044A>T in the promoter region of the TORC2 gene in the Qinchuan breed of cattle. Allelic and genotypic frequencies of these SNPs deviated from Hardy-Weinberg equilibrium (HWE) (P < 0.05). SNP1 genotype GG, SNP2 genotype CT and SNP3 genotype AT showed significantly (P <0.05) larger body measurement and improved carcass quality traits. Haplotype H1 (GCA) showed significantly (p<0.01) higher transcriptional activity (51.44%) followed by H4 (ATT) (34.13%) in bovine preadipocytes. The diplotypes HI-H3 (GG-CC-AT), H1-H2 (GG-CT-AT) and H3-H4 (GA-CT-TT) showed significant (P<0.01) associations with body measurement and improved carcass quality traits. Analysis of the relative mRNA expression level of the TORC2 gene in different tissues within two different age groups revealed a significant increase (P<0.01) in liver, small intestine, muscle and fat tissues with growth from calf stage to adult stage. We can conclude that variants mapped within TORC2 can be used in marker-assisted selection for carcass quality and body measurement traits in breed improvement programs of Qinchuan cattle. |
| first_indexed | 2024-12-19T02:24:31Z |
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| id | doaj.art-5cab2b2298734c858498490d0d05e56b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T02:24:31Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5cab2b2298734c858498490d0d05e56b2022-12-21T20:39:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01152e022725410.1371/journal.pone.0227254Genetic variants in the TORC2 gene promoter and their association with body measurement and carcass quality traits in Qinchuan cattle.Rajwali KhanSayed Haidar Abbas RazaHongfang GuoWang XiaoyuWu SenSyed Muhammad SuhailAbdur RahmanIrfan UllahAyman Hassan Abd El-AzizZeinab ManzariAkil AlshawiLinsen ZanThe TORC2 gene is responsible for nutrient metabolism, gluconeogenesis, myogenesis and adipogenesis through the PI3K-Akt, AMPK, glucagon and insulin resistance signaling pathways. Sequencing of PCR amplicons explored three novel SNPs at loci g.16534694G>A, g.16535011C>T, and g.16535044A>T in the promoter region of the TORC2 gene in the Qinchuan breed of cattle. Allelic and genotypic frequencies of these SNPs deviated from Hardy-Weinberg equilibrium (HWE) (P < 0.05). SNP1 genotype GG, SNP2 genotype CT and SNP3 genotype AT showed significantly (P <0.05) larger body measurement and improved carcass quality traits. Haplotype H1 (GCA) showed significantly (p<0.01) higher transcriptional activity (51.44%) followed by H4 (ATT) (34.13%) in bovine preadipocytes. The diplotypes HI-H3 (GG-CC-AT), H1-H2 (GG-CT-AT) and H3-H4 (GA-CT-TT) showed significant (P<0.01) associations with body measurement and improved carcass quality traits. Analysis of the relative mRNA expression level of the TORC2 gene in different tissues within two different age groups revealed a significant increase (P<0.01) in liver, small intestine, muscle and fat tissues with growth from calf stage to adult stage. We can conclude that variants mapped within TORC2 can be used in marker-assisted selection for carcass quality and body measurement traits in breed improvement programs of Qinchuan cattle.https://doi.org/10.1371/journal.pone.0227254 |
| spellingShingle | Rajwali Khan Sayed Haidar Abbas Raza Hongfang Guo Wang Xiaoyu Wu Sen Syed Muhammad Suhail Abdur Rahman Irfan Ullah Ayman Hassan Abd El-Aziz Zeinab Manzari Akil Alshawi Linsen Zan Genetic variants in the TORC2 gene promoter and their association with body measurement and carcass quality traits in Qinchuan cattle. PLoS ONE |
| title | Genetic variants in the TORC2 gene promoter and their association with body measurement and carcass quality traits in Qinchuan cattle. |
| title_full | Genetic variants in the TORC2 gene promoter and their association with body measurement and carcass quality traits in Qinchuan cattle. |
| title_fullStr | Genetic variants in the TORC2 gene promoter and their association with body measurement and carcass quality traits in Qinchuan cattle. |
| title_full_unstemmed | Genetic variants in the TORC2 gene promoter and their association with body measurement and carcass quality traits in Qinchuan cattle. |
| title_short | Genetic variants in the TORC2 gene promoter and their association with body measurement and carcass quality traits in Qinchuan cattle. |
| title_sort | genetic variants in the torc2 gene promoter and their association with body measurement and carcass quality traits in qinchuan cattle |
| url | https://doi.org/10.1371/journal.pone.0227254 |
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