Is it possible to obtain zero estimates of genetic diversity? A case study of the Nigerian indigenous goat breeds at the β-lactoglobulin gene locus
The current investigation was conducted to appraise the genetic diversity and genetic distance of three goat populations namely; Red Sokoto, Sahel and West African Dwarf (WAD), in Nigeria, making use of blood samples collected from 20, 20 and 20 individual from which blood DNAs were extraction, resp...
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| Format: | Article |
| Language: | English |
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Institute for Animal Husbandry, Belgrade
2017-01-01
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| Series: | Biotechnology in Animal Husbandry |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1450-9156/2017/1450-91561704375E.pdf |
| _version_ | 1811247087700934656 |
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| author | Ezewudo Emeka A. Abubakar Geka R. Egena Stephen-Sunday A. Alabi Olushola J. |
| author_facet | Ezewudo Emeka A. Abubakar Geka R. Egena Stephen-Sunday A. Alabi Olushola J. |
| author_sort | Ezewudo Emeka A. |
| collection | DOAJ |
| description | The current investigation was conducted to appraise the genetic diversity and genetic distance of three goat populations namely; Red Sokoto, Sahel and West African Dwarf (WAD), in Nigeria, making use of blood samples collected from 20, 20 and 20 individual from which blood DNAs were extraction, respectively. The DNAs extracted were used to study polymorphism at the β-lactoglobulin gene locus using RLFP-PCR process. Results revealed that the mean total number of alleles was 1 while the effective number of alleles was also 1. The percentage of polymorphic locus was 0% while Shannon's information index, observed homozygousity, expected heterozygosity, unbiased expected heterozygosity and inbreeding coefficient (F) were all observed to be 0.000. The pairwise Fst was 0.000 between all the breeds of goats. Variation within and between the populations of goats was 0% at p>0.05. The genetic distance between the goat breeds was 0.000. The present study revealed that RLFP-PCR may not be a powerful tool for the study of the β-lactoglobulin gene locus and hence other methodologies should be employed for a broader judgment on the genetic status of the goat population at the locus. |
| first_indexed | 2024-04-12T15:04:28Z |
| format | Article |
| id | doaj.art-5cad693e1e07451d88c6e463ade35d6a |
| institution | Directory Open Access Journal |
| issn | 1450-9156 2217-7140 |
| language | English |
| last_indexed | 2024-04-12T15:04:28Z |
| publishDate | 2017-01-01 |
| publisher | Institute for Animal Husbandry, Belgrade |
| record_format | Article |
| series | Biotechnology in Animal Husbandry |
| spelling | doaj.art-5cad693e1e07451d88c6e463ade35d6a2022-12-22T03:28:00ZengInstitute for Animal Husbandry, BelgradeBiotechnology in Animal Husbandry1450-91562217-71402017-01-013343753881450-91561704375EIs it possible to obtain zero estimates of genetic diversity? A case study of the Nigerian indigenous goat breeds at the β-lactoglobulin gene locusEzewudo Emeka A.0Abubakar Geka R.1Egena Stephen-Sunday A.2Alabi Olushola J.3Federal University of Technology, Department of Animal Production, Minna, Niger State, NigeriaFederal University of Technology, Department of Animal Production, Minna, Niger State, NigeriaFederal University of Technology, Department of Animal Production, Minna, Niger State, NigeriaFederal University of Technology, Department of Animal Production, Minna, Niger State, NigeriaThe current investigation was conducted to appraise the genetic diversity and genetic distance of three goat populations namely; Red Sokoto, Sahel and West African Dwarf (WAD), in Nigeria, making use of blood samples collected from 20, 20 and 20 individual from which blood DNAs were extraction, respectively. The DNAs extracted were used to study polymorphism at the β-lactoglobulin gene locus using RLFP-PCR process. Results revealed that the mean total number of alleles was 1 while the effective number of alleles was also 1. The percentage of polymorphic locus was 0% while Shannon's information index, observed homozygousity, expected heterozygosity, unbiased expected heterozygosity and inbreeding coefficient (F) were all observed to be 0.000. The pairwise Fst was 0.000 between all the breeds of goats. Variation within and between the populations of goats was 0% at p>0.05. The genetic distance between the goat breeds was 0.000. The present study revealed that RLFP-PCR may not be a powerful tool for the study of the β-lactoglobulin gene locus and hence other methodologies should be employed for a broader judgment on the genetic status of the goat population at the locus.https://scindeks-clanci.ceon.rs/data/pdf/1450-9156/2017/1450-91561704375E.pdfβ-lactoglobulinRLFP-PCRgenetic diversityNigerian indigenous goats and DNA |
| spellingShingle | Ezewudo Emeka A. Abubakar Geka R. Egena Stephen-Sunday A. Alabi Olushola J. Is it possible to obtain zero estimates of genetic diversity? A case study of the Nigerian indigenous goat breeds at the β-lactoglobulin gene locus Biotechnology in Animal Husbandry β-lactoglobulin RLFP-PCR genetic diversity Nigerian indigenous goats and DNA |
| title | Is it possible to obtain zero estimates of genetic diversity? A case study of the Nigerian indigenous goat breeds at the β-lactoglobulin gene locus |
| title_full | Is it possible to obtain zero estimates of genetic diversity? A case study of the Nigerian indigenous goat breeds at the β-lactoglobulin gene locus |
| title_fullStr | Is it possible to obtain zero estimates of genetic diversity? A case study of the Nigerian indigenous goat breeds at the β-lactoglobulin gene locus |
| title_full_unstemmed | Is it possible to obtain zero estimates of genetic diversity? A case study of the Nigerian indigenous goat breeds at the β-lactoglobulin gene locus |
| title_short | Is it possible to obtain zero estimates of genetic diversity? A case study of the Nigerian indigenous goat breeds at the β-lactoglobulin gene locus |
| title_sort | is it possible to obtain zero estimates of genetic diversity a case study of the nigerian indigenous goat breeds at the β lactoglobulin gene locus |
| topic | β-lactoglobulin RLFP-PCR genetic diversity Nigerian indigenous goats and DNA |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1450-9156/2017/1450-91561704375E.pdf |
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