Effects of whey peptide extract on the growth of probiotics and gut microbiota
Whey peptide extract with molecular weight below 1 kDa was investigated in microplate assay, and viable cells, as well as metabolic activity were determined to evaluate augmented growth of probiotic bacteria (Lactobacillus acidophilus and Bifidobacterium animalis). Results illustrated that whey pept...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-03-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464615005526 |
| _version_ | 1818647954070700032 |
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| author | Ya-Ju Yu Manuela Amorim Cláudia Marques Conceição Calhau Manuela Pintado |
| author_facet | Ya-Ju Yu Manuela Amorim Cláudia Marques Conceição Calhau Manuela Pintado |
| author_sort | Ya-Ju Yu |
| collection | DOAJ |
| description | Whey peptide extract with molecular weight below 1 kDa was investigated in microplate assay, and viable cells, as well as metabolic activity were determined to evaluate augmented growth of probiotic bacteria (Lactobacillus acidophilus and Bifidobacterium animalis). Results illustrated that whey peptide extract 1% (w/v) has the capacity to stimulate the proliferation of both probiotic bacteria tested, further supported by the faster generation of metabolic products. The effect of whey peptide extract on the modulation of gut microbiota was also examined in Wistar rats fed either with a standard or a high-fat diet, assessed via 16S ribosomal RNA expression of gut microbiota by quantitative PCR. Relative abundance of Lactobacillus spp., Bifidobacterium spp. and Bacteroidetes was significantly increased by whey peptide extract in rats fed with a standard diet. These results highlight an additional unexploited positive effect of whey peptide extract on gut microbiota modulation. |
| first_indexed | 2024-12-17T01:10:44Z |
| format | Article |
| id | doaj.art-5cb193061f4249bca33f23da2d00d23f |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T01:10:44Z |
| publishDate | 2016-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-5cb193061f4249bca33f23da2d00d23f2022-12-21T22:09:09ZengElsevierJournal of Functional Foods1756-46462016-03-0121507516Effects of whey peptide extract on the growth of probiotics and gut microbiotaYa-Ju Yu0Manuela Amorim1Cláudia Marques2Conceição Calhau3Manuela Pintado4Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, 4202-401 Porto, PortugalCentro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, 4202-401 Porto, PortugalDepartamento de Bioquímica, Faculdade de Medicina da Universidade do Porto, Al. Prof. Hernâni Monteiro, 4200-319 Porto, PortugalDepartamento de Bioquímica, Faculdade de Medicina da Universidade do Porto, Al. Prof. Hernâni Monteiro, 4200-319 Porto, Portugal; CINTESIS, Centro de Investigação em Tecnologias e Sistemas de Informação em Saúde, Al. Prof. Hernâni Monteiro, 4200-319 Porto, PortugalCentro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, 4202-401 Porto, Portugal; Corresponding author. Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, 4202-401 Porto, Portugal. Tel.: +351 225580097; fax: +351 22 558 00 01.Whey peptide extract with molecular weight below 1 kDa was investigated in microplate assay, and viable cells, as well as metabolic activity were determined to evaluate augmented growth of probiotic bacteria (Lactobacillus acidophilus and Bifidobacterium animalis). Results illustrated that whey peptide extract 1% (w/v) has the capacity to stimulate the proliferation of both probiotic bacteria tested, further supported by the faster generation of metabolic products. The effect of whey peptide extract on the modulation of gut microbiota was also examined in Wistar rats fed either with a standard or a high-fat diet, assessed via 16S ribosomal RNA expression of gut microbiota by quantitative PCR. Relative abundance of Lactobacillus spp., Bifidobacterium spp. and Bacteroidetes was significantly increased by whey peptide extract in rats fed with a standard diet. These results highlight an additional unexploited positive effect of whey peptide extract on gut microbiota modulation.http://www.sciencedirect.com/science/article/pii/S1756464615005526Gut microbiotaPrebioticsProbioticsWhey peptide extract |
| spellingShingle | Ya-Ju Yu Manuela Amorim Cláudia Marques Conceição Calhau Manuela Pintado Effects of whey peptide extract on the growth of probiotics and gut microbiota Journal of Functional Foods Gut microbiota Prebiotics Probiotics Whey peptide extract |
| title | Effects of whey peptide extract on the growth of probiotics and gut microbiota |
| title_full | Effects of whey peptide extract on the growth of probiotics and gut microbiota |
| title_fullStr | Effects of whey peptide extract on the growth of probiotics and gut microbiota |
| title_full_unstemmed | Effects of whey peptide extract on the growth of probiotics and gut microbiota |
| title_short | Effects of whey peptide extract on the growth of probiotics and gut microbiota |
| title_sort | effects of whey peptide extract on the growth of probiotics and gut microbiota |
| topic | Gut microbiota Prebiotics Probiotics Whey peptide extract |
| url | http://www.sciencedirect.com/science/article/pii/S1756464615005526 |
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