Cell Aggregate Assembly through Microengineering for Functional Tissue Emergence
Compared to cell suspensions or monolayers, 3D cell aggregates provide cellular interactions organized in space and heterogeneity that better resume the real organization of native tissues. They represent powerful tools to narrow down the gap between in vitro and in vivo models, thanks to their self...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
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| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/11/9/1394 |
| _version_ | 1797505319385956352 |
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| author | Gozde Eke Laurence Vaysse Xi Yao Mélanie Escudero Audrey Carrière Emmanuelle Trevisiol Christophe Vieu Christian Dani Louis Casteilla Laurent Malaquin |
| author_facet | Gozde Eke Laurence Vaysse Xi Yao Mélanie Escudero Audrey Carrière Emmanuelle Trevisiol Christophe Vieu Christian Dani Louis Casteilla Laurent Malaquin |
| author_sort | Gozde Eke |
| collection | DOAJ |
| description | Compared to cell suspensions or monolayers, 3D cell aggregates provide cellular interactions organized in space and heterogeneity that better resume the real organization of native tissues. They represent powerful tools to narrow down the gap between in vitro and in vivo models, thanks to their self-evolving capabilities. Recent strategies have demonstrated their potential as building blocks to generate microtissues. Developing specific methodologies capable of organizing these cell aggregates into 3D architectures and environments has become essential to convert them into functional microtissues adapted for regenerative medicine or pharmaceutical screening purposes. Although the techniques for producing individual cell aggregates have been on the market for over a decade, the methodology for engineering functional tissues starting from them is still a young and quickly evolving field of research. In this review, we first present a panorama of emerging cell aggregates microfabrication and assembly technologies. We further discuss the perspectives opened in the establishment of functional tissues with a specific focus on controlled architecture and heterogeneity to favor cell differentiation and proliferation. |
| first_indexed | 2024-03-10T04:16:55Z |
| format | Article |
| id | doaj.art-5cb56c8424e64dbb97c032230d779a70 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T04:16:55Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-5cb56c8424e64dbb97c032230d779a702023-11-23T07:58:36ZengMDPI AGCells2073-44092022-04-01119139410.3390/cells11091394Cell Aggregate Assembly through Microengineering for Functional Tissue EmergenceGozde Eke0Laurence Vaysse1Xi Yao2Mélanie Escudero3Audrey Carrière4Emmanuelle Trevisiol5Christophe Vieu6Christian Dani7Louis Casteilla8Laurent Malaquin9Laboratoire d’Analyse et d’Architecture des Systèmes, Centre National de la Recherche Scientifique (LAAS-CNRS), Université de Toulouse, INSA, UPS, 31400 Toulouse, FranceRESTORE Research Center, Université de Toulouse, INSERM 1301, CNRS 5070, EFS, ENVT, 31100 Toulouse, FranceInstitut de Biologie Valrose, Université Côte d’Azur, 06108 Nice, FranceRESTORE Research Center, Université de Toulouse, INSERM 1301, CNRS 5070, EFS, ENVT, 31100 Toulouse, FranceRESTORE Research Center, Université de Toulouse, INSERM 1301, CNRS 5070, EFS, ENVT, 31100 Toulouse, FranceLaboratoire d’Analyse et d’Architecture des Systèmes, Centre National de la Recherche Scientifique (LAAS-CNRS), Université de Toulouse, INSA, UPS, 31400 Toulouse, FranceLaboratoire d’Analyse et d’Architecture des Systèmes, Centre National de la Recherche Scientifique (LAAS-CNRS), Université de Toulouse, INSA, UPS, 31400 Toulouse, FranceInstitut de Biologie Valrose, Université Côte d’Azur, 06108 Nice, FranceRESTORE Research Center, Université de Toulouse, INSERM 1301, CNRS 5070, EFS, ENVT, 31100 Toulouse, FranceLaboratoire d’Analyse et d’Architecture des Systèmes, Centre National de la Recherche Scientifique (LAAS-CNRS), Université de Toulouse, INSA, UPS, 31400 Toulouse, FranceCompared to cell suspensions or monolayers, 3D cell aggregates provide cellular interactions organized in space and heterogeneity that better resume the real organization of native tissues. They represent powerful tools to narrow down the gap between in vitro and in vivo models, thanks to their self-evolving capabilities. Recent strategies have demonstrated their potential as building blocks to generate microtissues. Developing specific methodologies capable of organizing these cell aggregates into 3D architectures and environments has become essential to convert them into functional microtissues adapted for regenerative medicine or pharmaceutical screening purposes. Although the techniques for producing individual cell aggregates have been on the market for over a decade, the methodology for engineering functional tissues starting from them is still a young and quickly evolving field of research. In this review, we first present a panorama of emerging cell aggregates microfabrication and assembly technologies. We further discuss the perspectives opened in the establishment of functional tissues with a specific focus on controlled architecture and heterogeneity to favor cell differentiation and proliferation.https://www.mdpi.com/2073-4409/11/9/1394cell spheroidsorganoidscell aggregatesfunctional microtissuesmicrofabricationassembly technologies |
| spellingShingle | Gozde Eke Laurence Vaysse Xi Yao Mélanie Escudero Audrey Carrière Emmanuelle Trevisiol Christophe Vieu Christian Dani Louis Casteilla Laurent Malaquin Cell Aggregate Assembly through Microengineering for Functional Tissue Emergence Cells cell spheroids organoids cell aggregates functional microtissues microfabrication assembly technologies |
| title | Cell Aggregate Assembly through Microengineering for Functional Tissue Emergence |
| title_full | Cell Aggregate Assembly through Microengineering for Functional Tissue Emergence |
| title_fullStr | Cell Aggregate Assembly through Microengineering for Functional Tissue Emergence |
| title_full_unstemmed | Cell Aggregate Assembly through Microengineering for Functional Tissue Emergence |
| title_short | Cell Aggregate Assembly through Microengineering for Functional Tissue Emergence |
| title_sort | cell aggregate assembly through microengineering for functional tissue emergence |
| topic | cell spheroids organoids cell aggregates functional microtissues microfabrication assembly technologies |
| url | https://www.mdpi.com/2073-4409/11/9/1394 |
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