Genomic Characterization of Theileria luwenshuni Strain Cheeloo
ABSTRACT Theileria, a tick-borne intracellular protozoan, can cause infections of various livestock and wildlife around the world, posing a threat to veterinary health. Although more and more Theileria species have been identified, genomes have been available only from four Theileria species to date...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-08-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.00301-23 |
| _version_ | 1797741761729134592 |
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| author | Bai-Hui Wang Li-Feng Du Ming-Zhu Zhang Luo-Yuan Xia Cheng Li Zhe-Tao Lin Ning Wang Wan-Ying Gao Run-Ze Ye Jin-Yue Liu Xiao-Yu Han Wen-Qiang Shi Xiao-Yu Shi Jia-Fu Jiang Na Jia Xiao-Ming Cui Lin Zhao Wu-Chun Cao |
| author_facet | Bai-Hui Wang Li-Feng Du Ming-Zhu Zhang Luo-Yuan Xia Cheng Li Zhe-Tao Lin Ning Wang Wan-Ying Gao Run-Ze Ye Jin-Yue Liu Xiao-Yu Han Wen-Qiang Shi Xiao-Yu Shi Jia-Fu Jiang Na Jia Xiao-Ming Cui Lin Zhao Wu-Chun Cao |
| author_sort | Bai-Hui Wang |
| collection | DOAJ |
| description | ABSTRACT Theileria, a tick-borne intracellular protozoan, can cause infections of various livestock and wildlife around the world, posing a threat to veterinary health. Although more and more Theileria species have been identified, genomes have been available only from four Theileria species to date. Here, we assembled a whole genome of Theileria luwenshuni, an emerging Theileria, through next-generation sequencing of purified erythrocytes from the blood of a naturally infected goat. We designated it T. luwenshuni str. Cheeloo because its genome was assembled by the researchers at Cheeloo College of Medicine, Shandong University, China. The genome of T. lunwenshuni str. Cheeloo was the smallest in comparison with the other four Theileria species. T. luwenshuni str. Cheeloo possessed the fewest gene gains and gene family expansion. The protein count of each category was always comparable between T. luwenshuni str. Cheeloo and T. orientalis str. Shintoku in the Eukaryote Orthologs annotation, though there were remarkable differences in genome size. T. luwenshuni str. Cheeloo had lower counts than the other four Theileria species in most categories at level 3 of Gene Ontology annotation. Kyoto Encyclopedia of Genes and Genomes annotation revealed a loss of the c-Myb in T. luwenshuni str. Cheeloo. The infection rate of T. luwenshuni str. Cheeloo was up to 81.5% in a total of 54 goats from three flocks. The phylogenetic analyses based on both 18S rRNA and cox1 genes indicated that T. luwenshuni had relatively low diversity. The first characterization of the T. luwenshuni genome will promote better understanding of the emerging Theileria. IMPORTANCE Theileria has led to substantial economic losses in animal husbandry. Whole-genome sequencing data of the genus Theileria are currently limited, which has prohibited us from further understanding their molecular features. This work depicted whole-genome sequences of T. luwenshuni str. Cheeloo, an emerging Theileria species, and reported a high prevalence of T. luwenshuni str. Cheeloo infection in goats. The first assembly and characterization of T. luwenshuni genome will benefit exploring the infective and pathogenic mechanisms of the emerging Theileria to provide scientific basis for future control strategies of theileriosis. |
| first_indexed | 2024-03-12T14:31:19Z |
| format | Article |
| id | doaj.art-5cb7aa6c1a244972bed82956e8d78b74 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-12T14:31:19Z |
| publishDate | 2023-08-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-5cb7aa6c1a244972bed82956e8d78b742023-08-17T13:04:14ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972023-08-0111410.1128/spectrum.00301-23Genomic Characterization of Theileria luwenshuni Strain CheelooBai-Hui Wang0Li-Feng Du1Ming-Zhu Zhang2Luo-Yuan Xia3Cheng Li4Zhe-Tao Lin5Ning Wang6Wan-Ying Gao7Run-Ze Ye8Jin-Yue Liu9Xiao-Yu Han10Wen-Qiang Shi11Xiao-Yu Shi12Jia-Fu Jiang13Na Jia14Xiao-Ming Cui15Lin Zhao16Wu-Chun Cao17Institute of EcoHealth, School of Public Health, Shandong University, Jinan, Shandong, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Shandong University, Jinan, Shandong, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Shandong University, Jinan, Shandong, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Shandong University, Jinan, Shandong, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Shandong University, Jinan, Shandong, People’s Republic of ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Shandong University, Jinan, Shandong, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Shandong University, Jinan, Shandong, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Shandong University, Jinan, Shandong, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Shandong University, Jinan, Shandong, People’s Republic of ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Shandong University, Jinan, Shandong, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Shandong University, Jinan, Shandong, People’s Republic of ChinaABSTRACT Theileria, a tick-borne intracellular protozoan, can cause infections of various livestock and wildlife around the world, posing a threat to veterinary health. Although more and more Theileria species have been identified, genomes have been available only from four Theileria species to date. Here, we assembled a whole genome of Theileria luwenshuni, an emerging Theileria, through next-generation sequencing of purified erythrocytes from the blood of a naturally infected goat. We designated it T. luwenshuni str. Cheeloo because its genome was assembled by the researchers at Cheeloo College of Medicine, Shandong University, China. The genome of T. lunwenshuni str. Cheeloo was the smallest in comparison with the other four Theileria species. T. luwenshuni str. Cheeloo possessed the fewest gene gains and gene family expansion. The protein count of each category was always comparable between T. luwenshuni str. Cheeloo and T. orientalis str. Shintoku in the Eukaryote Orthologs annotation, though there were remarkable differences in genome size. T. luwenshuni str. Cheeloo had lower counts than the other four Theileria species in most categories at level 3 of Gene Ontology annotation. Kyoto Encyclopedia of Genes and Genomes annotation revealed a loss of the c-Myb in T. luwenshuni str. Cheeloo. The infection rate of T. luwenshuni str. Cheeloo was up to 81.5% in a total of 54 goats from three flocks. The phylogenetic analyses based on both 18S rRNA and cox1 genes indicated that T. luwenshuni had relatively low diversity. The first characterization of the T. luwenshuni genome will promote better understanding of the emerging Theileria. IMPORTANCE Theileria has led to substantial economic losses in animal husbandry. Whole-genome sequencing data of the genus Theileria are currently limited, which has prohibited us from further understanding their molecular features. This work depicted whole-genome sequences of T. luwenshuni str. Cheeloo, an emerging Theileria species, and reported a high prevalence of T. luwenshuni str. Cheeloo infection in goats. The first assembly and characterization of T. luwenshuni genome will benefit exploring the infective and pathogenic mechanisms of the emerging Theileria to provide scientific basis for future control strategies of theileriosis.https://journals.asm.org/doi/10.1128/spectrum.00301-23Theileria luwenshuni strain Cheeloowhole-genome sequenceFISHgenomic characteristicsphylogenetic analysis |
| spellingShingle | Bai-Hui Wang Li-Feng Du Ming-Zhu Zhang Luo-Yuan Xia Cheng Li Zhe-Tao Lin Ning Wang Wan-Ying Gao Run-Ze Ye Jin-Yue Liu Xiao-Yu Han Wen-Qiang Shi Xiao-Yu Shi Jia-Fu Jiang Na Jia Xiao-Ming Cui Lin Zhao Wu-Chun Cao Genomic Characterization of Theileria luwenshuni Strain Cheeloo Microbiology Spectrum Theileria luwenshuni strain Cheeloo whole-genome sequence FISH genomic characteristics phylogenetic analysis |
| title | Genomic Characterization of Theileria luwenshuni Strain Cheeloo |
| title_full | Genomic Characterization of Theileria luwenshuni Strain Cheeloo |
| title_fullStr | Genomic Characterization of Theileria luwenshuni Strain Cheeloo |
| title_full_unstemmed | Genomic Characterization of Theileria luwenshuni Strain Cheeloo |
| title_short | Genomic Characterization of Theileria luwenshuni Strain Cheeloo |
| title_sort | genomic characterization of theileria luwenshuni strain cheeloo |
| topic | Theileria luwenshuni strain Cheeloo whole-genome sequence FISH genomic characteristics phylogenetic analysis |
| url | https://journals.asm.org/doi/10.1128/spectrum.00301-23 |
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