Hydroxyapatite from biowaste for biomedical applications: obtainment, characterization and in vitro assays
The use of biogenic residues to obtain calcium phosphate compounds, mainly hydroxyapatite (HAp), is an excellent alternative to reduce costs in obtaining biomaterials. The purpose of this study was to obtain hydroxyapatite from fish bones and hen eggshells by alkaline hydrolysis and precipitation, r...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol)
2023-06-01
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| Series: | Materials Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392023000100272&tlng=en |
| _version_ | 1827908979050676224 |
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| author | Marla Karolyne dos Santos Horta Cecília Westin Daniel Navarro da Rocha José Brant de Campos Rodrigo Fernandes Magalhães de Souza Marilza Sampaio Aguilar Francisco José Moura |
| author_facet | Marla Karolyne dos Santos Horta Cecília Westin Daniel Navarro da Rocha José Brant de Campos Rodrigo Fernandes Magalhães de Souza Marilza Sampaio Aguilar Francisco José Moura |
| author_sort | Marla Karolyne dos Santos Horta |
| collection | DOAJ |
| description | The use of biogenic residues to obtain calcium phosphate compounds, mainly hydroxyapatite (HAp), is an excellent alternative to reduce costs in obtaining biomaterials. The purpose of this study was to obtain hydroxyapatite from fish bones and hen eggshells by alkaline hydrolysis and precipitation, respectively, and compare them to HAp obtained from calcium commercial source based on physical-chemical properties and in vitro assays. The biomaterials obtained were characterized by X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Brunauer-Emmett-Teller (BET) method and Fourier Transform Infrared Spectroscopy (FTIR). The XRD results revealed the presence of the main characteristic peaks of single HAp phase and FTIR analysis showed various functional groups, such as PO43-, CO3-2 and OH-, confirming the presence of HAp. SEM observations of the synthesized HAp showed a rod-like and spherical-like morphology. The cell viability and bioactivity of the materials were evaluated by rezasurin reduction and McCoy medium assays, respectively. The biomaterials obtained had no toxic effect and the sample obtained from fish bone was more bioactive when compared to the others. Therefore, the biowastes can be used as an alternative source of calcium in the synthesis of hydroxyapatite with promising properties for application in the biomedical area. |
| first_indexed | 2024-03-13T01:30:57Z |
| format | Article |
| id | doaj.art-5cb7caea29644b9bb235db3c8e0974b2 |
| institution | Directory Open Access Journal |
| issn | 1516-1439 |
| language | English |
| last_indexed | 2024-03-13T01:30:57Z |
| publishDate | 2023-06-01 |
| publisher | Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol) |
| record_format | Article |
| series | Materials Research |
| spelling | doaj.art-5cb7caea29644b9bb235db3c8e0974b22023-07-04T07:43:21ZengAssociação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol)Materials Research1516-14392023-06-012610.1590/1980-5373-mr-2022-0466Hydroxyapatite from biowaste for biomedical applications: obtainment, characterization and in vitro assaysMarla Karolyne dos Santos Hortahttps://orcid.org/0000-0002-4210-273XCecília WestinDaniel Navarro da RochaJosé Brant de Camposhttps://orcid.org/0000-0001-7425-401XRodrigo Fernandes Magalhães de Souzahttps://orcid.org/0000-0002-3468-686XMarilza Sampaio Aguilarhttps://orcid.org/0000-0002-0966-5936Francisco José MouraThe use of biogenic residues to obtain calcium phosphate compounds, mainly hydroxyapatite (HAp), is an excellent alternative to reduce costs in obtaining biomaterials. The purpose of this study was to obtain hydroxyapatite from fish bones and hen eggshells by alkaline hydrolysis and precipitation, respectively, and compare them to HAp obtained from calcium commercial source based on physical-chemical properties and in vitro assays. The biomaterials obtained were characterized by X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Brunauer-Emmett-Teller (BET) method and Fourier Transform Infrared Spectroscopy (FTIR). The XRD results revealed the presence of the main characteristic peaks of single HAp phase and FTIR analysis showed various functional groups, such as PO43-, CO3-2 and OH-, confirming the presence of HAp. SEM observations of the synthesized HAp showed a rod-like and spherical-like morphology. The cell viability and bioactivity of the materials were evaluated by rezasurin reduction and McCoy medium assays, respectively. The biomaterials obtained had no toxic effect and the sample obtained from fish bone was more bioactive when compared to the others. Therefore, the biowastes can be used as an alternative source of calcium in the synthesis of hydroxyapatite with promising properties for application in the biomedical area.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392023000100272&tlng=enHydroxyapatitebiomaterialbiowasteeggshellfishbonerecycling |
| spellingShingle | Marla Karolyne dos Santos Horta Cecília Westin Daniel Navarro da Rocha José Brant de Campos Rodrigo Fernandes Magalhães de Souza Marilza Sampaio Aguilar Francisco José Moura Hydroxyapatite from biowaste for biomedical applications: obtainment, characterization and in vitro assays Materials Research Hydroxyapatite biomaterial biowaste eggshell fishbone recycling |
| title | Hydroxyapatite from biowaste for biomedical applications: obtainment, characterization and in vitro assays |
| title_full | Hydroxyapatite from biowaste for biomedical applications: obtainment, characterization and in vitro assays |
| title_fullStr | Hydroxyapatite from biowaste for biomedical applications: obtainment, characterization and in vitro assays |
| title_full_unstemmed | Hydroxyapatite from biowaste for biomedical applications: obtainment, characterization and in vitro assays |
| title_short | Hydroxyapatite from biowaste for biomedical applications: obtainment, characterization and in vitro assays |
| title_sort | hydroxyapatite from biowaste for biomedical applications obtainment characterization and in vitro assays |
| topic | Hydroxyapatite biomaterial biowaste eggshell fishbone recycling |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392023000100272&tlng=en |
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