Insights into Mechanical Behavior and Biological Properties of Chia Seed Mucilage Hydrogels
In this contribution we report insights on the rheological properties of chia (<i>Salvia hispanica</i>) seed mucilage hydrogels. Creep experiments performed in steady state conditions allowed calculation of Newtonian viscosities for chia hydrogels with different polymer concentration, po...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | Gels |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2310-2861/7/2/47 |
| _version_ | 1797537049879773184 |
|---|---|
| author | Pasquale Sacco Sara Lipari Michela Cok Matilde Colella Eleonora Marsich Francesco Lopez Ivan Donati |
| author_facet | Pasquale Sacco Sara Lipari Michela Cok Matilde Colella Eleonora Marsich Francesco Lopez Ivan Donati |
| author_sort | Pasquale Sacco |
| collection | DOAJ |
| description | In this contribution we report insights on the rheological properties of chia (<i>Salvia hispanica</i>) seed mucilage hydrogels. Creep experiments performed in steady state conditions allowed calculation of Newtonian viscosities for chia hydrogels with different polymer concentration, pointing at inter-chain interactions as the main responsible for the different behavior toward network slipping under constant stress. A combination of oscillatory frequency and stress sweep tests highlighted a moderate effect of temperature in influencing hydrogel mechanics. The latter results prompted us to investigate potential biological functions for this set of biomaterials. Lactate Dehydrogenase assay proved the lack of cytotoxicity of chia suspensions toward Human Mesenchymal Stem Cells from adipose tissue used here as a cell model. Differentiation experiments were finally undertaken to verify the influence of chia samples on osteo-induction triggered by chemical differentiation factors. Alkaline Phosphatase enzyme activity assay and Alizarin red staining demonstrated that chia mucilage did not alter in vitro stem cell differentiation. Collectively, this set of experiments revealed an almost inert role associated with chia suspensions, indicating a possible application of chia-based networks as scaffold models to study osteogenesis in vitro. |
| first_indexed | 2024-03-10T12:09:36Z |
| format | Article |
| id | doaj.art-5cba2b0991b94bba9ea5907ea4e19f2e |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-10T12:09:36Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-5cba2b0991b94bba9ea5907ea4e19f2e2023-11-21T16:18:03ZengMDPI AGGels2310-28612021-04-01724710.3390/gels7020047Insights into Mechanical Behavior and Biological Properties of Chia Seed Mucilage HydrogelsPasquale Sacco0Sara Lipari1Michela Cok2Matilde Colella3Eleonora Marsich4Francesco Lopez5Ivan Donati6Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, Via Licio Giorgieri 5, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, Via Licio Giorgieri 5, 34127 Trieste, ItalyDepartment of Biosciences, Biotechnology and Biopharmaceutics, University of Bari “Aldo Moro”, Via Orabona, 4, 70126 Bari, ItalyDepartment of Medicine, Surgery and Health Sciences, University of Trieste, Piazza dell’Ospitale 1, 34129 Trieste, ItalyDepartment of Agricultural, Environmental and Food Sciences (DiAAA) and Center for Colloid and Surface Science (CSGI), University of Molise, Via De Sanctis, 86100 Campobasso, ItalyDepartment of Life Sciences, University of Trieste, Via Licio Giorgieri 5, 34127 Trieste, ItalyIn this contribution we report insights on the rheological properties of chia (<i>Salvia hispanica</i>) seed mucilage hydrogels. Creep experiments performed in steady state conditions allowed calculation of Newtonian viscosities for chia hydrogels with different polymer concentration, pointing at inter-chain interactions as the main responsible for the different behavior toward network slipping under constant stress. A combination of oscillatory frequency and stress sweep tests highlighted a moderate effect of temperature in influencing hydrogel mechanics. The latter results prompted us to investigate potential biological functions for this set of biomaterials. Lactate Dehydrogenase assay proved the lack of cytotoxicity of chia suspensions toward Human Mesenchymal Stem Cells from adipose tissue used here as a cell model. Differentiation experiments were finally undertaken to verify the influence of chia samples on osteo-induction triggered by chemical differentiation factors. Alkaline Phosphatase enzyme activity assay and Alizarin red staining demonstrated that chia mucilage did not alter in vitro stem cell differentiation. Collectively, this set of experiments revealed an almost inert role associated with chia suspensions, indicating a possible application of chia-based networks as scaffold models to study osteogenesis in vitro.https://www.mdpi.com/2310-2861/7/2/47chia seedsmucilagehydrogelsmechanical behaviorcytotoxicityosteogenesis |
| spellingShingle | Pasquale Sacco Sara Lipari Michela Cok Matilde Colella Eleonora Marsich Francesco Lopez Ivan Donati Insights into Mechanical Behavior and Biological Properties of Chia Seed Mucilage Hydrogels Gels chia seeds mucilage hydrogels mechanical behavior cytotoxicity osteogenesis |
| title | Insights into Mechanical Behavior and Biological Properties of Chia Seed Mucilage Hydrogels |
| title_full | Insights into Mechanical Behavior and Biological Properties of Chia Seed Mucilage Hydrogels |
| title_fullStr | Insights into Mechanical Behavior and Biological Properties of Chia Seed Mucilage Hydrogels |
| title_full_unstemmed | Insights into Mechanical Behavior and Biological Properties of Chia Seed Mucilage Hydrogels |
| title_short | Insights into Mechanical Behavior and Biological Properties of Chia Seed Mucilage Hydrogels |
| title_sort | insights into mechanical behavior and biological properties of chia seed mucilage hydrogels |
| topic | chia seeds mucilage hydrogels mechanical behavior cytotoxicity osteogenesis |
| url | https://www.mdpi.com/2310-2861/7/2/47 |
| work_keys_str_mv | AT pasqualesacco insightsintomechanicalbehaviorandbiologicalpropertiesofchiaseedmucilagehydrogels AT saralipari insightsintomechanicalbehaviorandbiologicalpropertiesofchiaseedmucilagehydrogels AT michelacok insightsintomechanicalbehaviorandbiologicalpropertiesofchiaseedmucilagehydrogels AT matildecolella insightsintomechanicalbehaviorandbiologicalpropertiesofchiaseedmucilagehydrogels AT eleonoramarsich insightsintomechanicalbehaviorandbiologicalpropertiesofchiaseedmucilagehydrogels AT francescolopez insightsintomechanicalbehaviorandbiologicalpropertiesofchiaseedmucilagehydrogels AT ivandonati insightsintomechanicalbehaviorandbiologicalpropertiesofchiaseedmucilagehydrogels |