Characterization and Application of the Sugar Transporter Zmo0293 from <i>Zymomonas mobilis</i>
<i>Zymomonas mobilis</i> is a natural ethanologen with many desirable characteristics, which makes it an ideal industrial microbial biocatalyst for the commercial production of desirable bioproducts. Sugar transporters are responsible for the import of substrate sugars and the conversion...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/6/5888 |
| Summary: | <i>Zymomonas mobilis</i> is a natural ethanologen with many desirable characteristics, which makes it an ideal industrial microbial biocatalyst for the commercial production of desirable bioproducts. Sugar transporters are responsible for the import of substrate sugars and the conversion of ethanol and other products. Glucose-facilitated diffusion protein Glf is responsible for facilitating the diffusion of glucose uptake in <i>Z. mobilis</i>. However, another sugar transporter-encoded gene, <i>ZMO0293</i>, is poorly characterized. We employed gene deletion and heterologous expression mediated by the CRISPR/Cas method to investigate the role of <i>ZMO0293</i>. The results showed that deletion of the <i>ZMO0293</i> gene slowed growth and reduced ethanol production and the activities of key enzymes involved in glucose metabolism in the presence of high concentrations of glucose. Moreover, <i>ZMO0293</i> deletion caused different transcriptional changes in some genes of the Entner Doudoroff (ED) pathway in the ZM4-Δ<i>ZM0293</i> strain but not in ZM4 cells. The integrated expression of <i>ZMO0293</i> restored the growth of the glucose uptake-defective strain <i>Escherichia coli</i> BL21(DE3)-Δ<i>ptsG</i>. This study reveals the function of the <i>ZMO0293</i> gene in <i>Z. mobilis</i> in response to high concentrations of glucose and provides a new biological part for synthetic biology. |
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| ISSN: | 1661-6596 1422-0067 |