Stable contacts of naïve CD4 T cells with migratory dendritic cells are ICAM-1-dependent but dispensable for proliferation in vivo
It is unclear if naïve T cells require dendritic cell ICAMs to proliferate inside lymph nodes. To check if and when CD4 lymphocytes use ICAMs on migratory DCs, wild-type and ICAM-1 and 2 double knock out bone marrow-derived DCs pulsed with saturating levels of an OT-II transgene-specific ovalbumin-d...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Cell Adhesion & Migration |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19336918.2019.1644857 |
| _version_ | 1818310760707653632 |
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| author | Stav Kozlovski Ofir Atrakchi Sara W Feigelson Ziv Shulman Ronen Alon |
| author_facet | Stav Kozlovski Ofir Atrakchi Sara W Feigelson Ziv Shulman Ronen Alon |
| author_sort | Stav Kozlovski |
| collection | DOAJ |
| description | It is unclear if naïve T cells require dendritic cell ICAMs to proliferate inside lymph nodes. To check if and when CD4 lymphocytes use ICAMs on migratory DCs, wild-type and ICAM-1 and 2 double knock out bone marrow-derived DCs pulsed with saturating levels of an OT-II transgene-specific ovalbumin-derived peptide were co-transferred into skin-draining lymph nodes. Intravital imaging of OT-II lymphocytes entering these lymph nodes revealed that ICAM-1 and −2 deficient migratory DCs formed fewer stable conjugates with OT-II lymphocytes but promoted normal T cell proliferation. DC ICAMs were also not required for unstable TCR-dependent lymphocyte arrests on antigen presenting migratory DCs. Thus, rare antigen-stimulated ICAM-stabilized T-DC conjugates are dispensable for CD4 lymphocyte proliferation inside lymph nodes. |
| first_indexed | 2024-12-13T07:51:11Z |
| format | Article |
| id | doaj.art-5cc387acedf745ec8c71bb10581837c8 |
| institution | Directory Open Access Journal |
| issn | 1933-6918 1933-6926 |
| language | English |
| last_indexed | 2024-12-13T07:51:11Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cell Adhesion & Migration |
| spelling | doaj.art-5cc387acedf745ec8c71bb10581837c82022-12-21T23:54:40ZengTaylor & Francis GroupCell Adhesion & Migration1933-69181933-69262019-01-0113131432010.1080/19336918.2019.16448571644857Stable contacts of naïve CD4 T cells with migratory dendritic cells are ICAM-1-dependent but dispensable for proliferation in vivoStav Kozlovski0Ofir Atrakchi1Sara W Feigelson2Ziv Shulman3Ronen Alon4Weizmann Institute of ScienceWeizmann Institute of ScienceWeizmann Institute of ScienceWeizmann Institute of ScienceWeizmann Institute of ScienceIt is unclear if naïve T cells require dendritic cell ICAMs to proliferate inside lymph nodes. To check if and when CD4 lymphocytes use ICAMs on migratory DCs, wild-type and ICAM-1 and 2 double knock out bone marrow-derived DCs pulsed with saturating levels of an OT-II transgene-specific ovalbumin-derived peptide were co-transferred into skin-draining lymph nodes. Intravital imaging of OT-II lymphocytes entering these lymph nodes revealed that ICAM-1 and −2 deficient migratory DCs formed fewer stable conjugates with OT-II lymphocytes but promoted normal T cell proliferation. DC ICAMs were also not required for unstable TCR-dependent lymphocyte arrests on antigen presenting migratory DCs. Thus, rare antigen-stimulated ICAM-stabilized T-DC conjugates are dispensable for CD4 lymphocyte proliferation inside lymph nodes.http://dx.doi.org/10.1080/19336918.2019.1644857lymph nodesdendritic cellsintegrinst cell activationvaccination |
| spellingShingle | Stav Kozlovski Ofir Atrakchi Sara W Feigelson Ziv Shulman Ronen Alon Stable contacts of naïve CD4 T cells with migratory dendritic cells are ICAM-1-dependent but dispensable for proliferation in vivo Cell Adhesion & Migration lymph nodes dendritic cells integrins t cell activation vaccination |
| title | Stable contacts of naïve CD4 T cells with migratory dendritic cells are ICAM-1-dependent but dispensable for proliferation in vivo |
| title_full | Stable contacts of naïve CD4 T cells with migratory dendritic cells are ICAM-1-dependent but dispensable for proliferation in vivo |
| title_fullStr | Stable contacts of naïve CD4 T cells with migratory dendritic cells are ICAM-1-dependent but dispensable for proliferation in vivo |
| title_full_unstemmed | Stable contacts of naïve CD4 T cells with migratory dendritic cells are ICAM-1-dependent but dispensable for proliferation in vivo |
| title_short | Stable contacts of naïve CD4 T cells with migratory dendritic cells are ICAM-1-dependent but dispensable for proliferation in vivo |
| title_sort | stable contacts of naive cd4 t cells with migratory dendritic cells are icam 1 dependent but dispensable for proliferation in vivo |
| topic | lymph nodes dendritic cells integrins t cell activation vaccination |
| url | http://dx.doi.org/10.1080/19336918.2019.1644857 |
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