Effects of casein kinase 2 interacting protein-1 on the osteogenic differentiation ability of human periodontal ligament stem cells
Objective To investigate the effects of casein kinase 2 interacting protein-1 (CKIP-1) on the osteogenic differentiation ability of human periodontal ligament stem cells (hPDLSCs). Methods The hPDLSCs were obtained by primary culture with periodontal ligament tissues that were collected from normal...
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| Format: | Article |
| Language: | zho |
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Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
2020-07-01
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| Series: | 口腔疾病防治 |
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| Online Access: | http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2020.07.003 |
| _version_ | 1811206501964972032 |
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| author | QIN Qing SONG Yang LIU Jia LI Qiang |
| author_facet | QIN Qing SONG Yang LIU Jia LI Qiang |
| author_sort | QIN Qing |
| collection | DOAJ |
| description | Objective To investigate the effects of casein kinase 2 interacting protein-1 (CKIP-1) on the osteogenic differentiation ability of human periodontal ligament stem cells (hPDLSCs). Methods The hPDLSCs were obtained by primary culture with periodontal ligament tissues that were collected from normal humans. Then, a lentiviral vector containing a CKIP-1-specific siRNA sequence was constructed, and the transcriptional level of CKIP-1 in hPDLSCs was downregulated after vector infection. The P4 cells were divided into four groups: the control group, negative control group (infected with a control vector), CKIP-siRNA group (infected by a CKIP-1 siRNA lentivirus) and CKIP-1 group (infected by a CKIP-1 overexpression virus). All of the cells were cultured under osteogenic induction for 21 days. Then, alizarin red staining and quantitative determination were performed to detect the osteogenic differentiation ability of the hPDLSCs. In addition, qPCR was used to detect the transcriptional level of osteogenesis-related regulatory factors, such as Runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), osteocalcin (OCN), and receptor activator of nuclear factor kappa-B ligand (RANKL), and the osteogenesis-related regulatory factors of the bone morphogenetic protein (BMP) signaling pathway. Results There were no differences in the indexes between the negative control group and the control group (P > 0.05). Compared with the negative control group, the CKIP-siRNA group demonstrated more mineralized nodules (P < 0.05), significantly increased calcium salt deposition (P < 0.05), and increased mRNA levels of osteogenesis-related regulatory factors, such as Runx2 , ALP, OCN, and RANKL, and the osteogenesis-related regulatory factors of BMP signaling pathway (P < 0.05). Conclusion Downregulation of CKIP-1 could promote the osteogenic differentiation of hPDLSCs, which is related to the transcription level of osteogenic-related regulatory factors. |
| first_indexed | 2024-04-12T03:49:42Z |
| format | Article |
| id | doaj.art-5cc40226ed424101856824f3f024e045 |
| institution | Directory Open Access Journal |
| issn | 2096-1456 2096-1456 |
| language | zho |
| last_indexed | 2024-04-12T03:49:42Z |
| publishDate | 2020-07-01 |
| publisher | Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases |
| record_format | Article |
| series | 口腔疾病防治 |
| spelling | doaj.art-5cc40226ed424101856824f3f024e0452022-12-22T03:49:02ZzhoEditorial Department of Journal of Prevention and Treatment for Stomatological Diseases口腔疾病防治2096-14562096-14562020-07-0128742142610.12016/j.issn.2096-1456.2020.07.003Effects of casein kinase 2 interacting protein-1 on the osteogenic differentiation ability of human periodontal ligament stem cellsQIN Qing0SONG Yang1 LIU Jia2LI Qiang3Department of Dental, The Second Affiliated Hospital of Xi′an Jiaotong University (Xibei Hospital)Department of Stomatology, the 986 Hospital of PLAState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shanxi Clinical Research Center for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical UniversityState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shanxi International Joint Research Center for Oral Diseases, Department of General Dentistry & Emergency, School of Stomatology, The Fourth Military Medical UniversityObjective To investigate the effects of casein kinase 2 interacting protein-1 (CKIP-1) on the osteogenic differentiation ability of human periodontal ligament stem cells (hPDLSCs). Methods The hPDLSCs were obtained by primary culture with periodontal ligament tissues that were collected from normal humans. Then, a lentiviral vector containing a CKIP-1-specific siRNA sequence was constructed, and the transcriptional level of CKIP-1 in hPDLSCs was downregulated after vector infection. The P4 cells were divided into four groups: the control group, negative control group (infected with a control vector), CKIP-siRNA group (infected by a CKIP-1 siRNA lentivirus) and CKIP-1 group (infected by a CKIP-1 overexpression virus). All of the cells were cultured under osteogenic induction for 21 days. Then, alizarin red staining and quantitative determination were performed to detect the osteogenic differentiation ability of the hPDLSCs. In addition, qPCR was used to detect the transcriptional level of osteogenesis-related regulatory factors, such as Runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), osteocalcin (OCN), and receptor activator of nuclear factor kappa-B ligand (RANKL), and the osteogenesis-related regulatory factors of the bone morphogenetic protein (BMP) signaling pathway. Results There were no differences in the indexes between the negative control group and the control group (P > 0.05). Compared with the negative control group, the CKIP-siRNA group demonstrated more mineralized nodules (P < 0.05), significantly increased calcium salt deposition (P < 0.05), and increased mRNA levels of osteogenesis-related regulatory factors, such as Runx2 , ALP, OCN, and RANKL, and the osteogenesis-related regulatory factors of BMP signaling pathway (P < 0.05). Conclusion Downregulation of CKIP-1 could promote the osteogenic differentiation of hPDLSCs, which is related to the transcription level of osteogenic-related regulatory factors.http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2020.07.003periodontitisperiodontal bone losscasein kinase 2 interacting protein-1human periodontal ligament stem cellsosteogenic differentiationsirna interferencerunt-related transcription factor 2alkaline phosphataseosteocalcinreceptor activator ofnuclear factor kappa-b ligandbone morphogenetic protein signaling pathway |
| spellingShingle | QIN Qing SONG Yang LIU Jia LI Qiang Effects of casein kinase 2 interacting protein-1 on the osteogenic differentiation ability of human periodontal ligament stem cells 口腔疾病防治 periodontitis periodontal bone loss casein kinase 2 interacting protein-1 human periodontal ligament stem cells osteogenic differentiation sirna interference runt-related transcription factor 2 alkaline phosphatase osteocalcin receptor activator ofnuclear factor kappa-b ligand bone morphogenetic protein signaling pathway |
| title | Effects of casein kinase 2 interacting protein-1 on the osteogenic differentiation ability of human periodontal ligament stem cells |
| title_full | Effects of casein kinase 2 interacting protein-1 on the osteogenic differentiation ability of human periodontal ligament stem cells |
| title_fullStr | Effects of casein kinase 2 interacting protein-1 on the osteogenic differentiation ability of human periodontal ligament stem cells |
| title_full_unstemmed | Effects of casein kinase 2 interacting protein-1 on the osteogenic differentiation ability of human periodontal ligament stem cells |
| title_short | Effects of casein kinase 2 interacting protein-1 on the osteogenic differentiation ability of human periodontal ligament stem cells |
| title_sort | effects of casein kinase 2 interacting protein 1 on the osteogenic differentiation ability of human periodontal ligament stem cells |
| topic | periodontitis periodontal bone loss casein kinase 2 interacting protein-1 human periodontal ligament stem cells osteogenic differentiation sirna interference runt-related transcription factor 2 alkaline phosphatase osteocalcin receptor activator ofnuclear factor kappa-b ligand bone morphogenetic protein signaling pathway |
| url | http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2020.07.003 |
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