Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast Development
The generation of oxygen and organic matter in plants mainly depends on photosynthesis, which directly affects plant growth and development. The chloroplast is the main organelle in which photosynthesis occurs. In this study, a Glycine max pale green leaf 3-1 (Gmpgl3-1) mutant was isolated from the...
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.892077/full |
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| author | Hui Yu Qiushi Wang Zhirui Zhang Zhirui Zhang Tao Wu Tao Wu Xinjing Yang Xinjing Yang Xiaobin Zhu Xiaobin Zhu Yongheng Ye Yongheng Ye Jiantian Leng Suxin Yang Xianzhong Feng Xianzhong Feng Xianzhong Feng |
| author_facet | Hui Yu Qiushi Wang Zhirui Zhang Zhirui Zhang Tao Wu Tao Wu Xinjing Yang Xinjing Yang Xiaobin Zhu Xiaobin Zhu Yongheng Ye Yongheng Ye Jiantian Leng Suxin Yang Xianzhong Feng Xianzhong Feng Xianzhong Feng |
| author_sort | Hui Yu |
| collection | DOAJ |
| description | The generation of oxygen and organic matter in plants mainly depends on photosynthesis, which directly affects plant growth and development. The chloroplast is the main organelle in which photosynthesis occurs. In this study, a Glycine max pale green leaf 3-1 (Gmpgl3-1) mutant was isolated from the soybean mutagenized population. The Gmpgl3-1 mutant presented with decreased chlorophyll contents, reduced chloroplast stroma thylakoids, reduced yields, and decreased numbers of pods per plant. Bulked segregant analysis (BSA) together with map-based cloning revealed a single-nucleotide non-synonymous mutation at the 341st nucleotide of the first exon of the chloroplast development-related GmTic110a gene. The phenotype of the knockout plants was the same as that of the mutant. The GmTic110a gene was highly expressed in the leaves at various developmental stages, and its protein was localized to the inner chloroplast membrane. Split luciferase complementation assays and coimmunoprecipitation (co-IP) experiments revealed that GmTic110a interacted with GmTic20, GmTic40a, and GmTic40b in tobacco leaves. These results indicated that the GmTic110a gene plays an important role in chloroplast development. |
| first_indexed | 2024-04-12T17:36:38Z |
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| id | doaj.art-5cc605b5ad77419cb1ec5d029aaeccc5 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T17:36:38Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-5cc605b5ad77419cb1ec5d029aaeccc52022-12-22T03:22:56ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-05-011310.3389/fpls.2022.892077892077Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast DevelopmentHui Yu0Qiushi Wang1Zhirui Zhang2Zhirui Zhang3Tao Wu4Tao Wu5Xinjing Yang6Xinjing Yang7Xiaobin Zhu8Xiaobin Zhu9Yongheng Ye10Yongheng Ye11Jiantian Leng12Suxin Yang13Xianzhong Feng14Xianzhong Feng15Xianzhong Feng16Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaCollege of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaCollege of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaCollege of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaCollege of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaCollege of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaCollege of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, ChinaZhejiang Lab, Hangzhou, ChinaThe generation of oxygen and organic matter in plants mainly depends on photosynthesis, which directly affects plant growth and development. The chloroplast is the main organelle in which photosynthesis occurs. In this study, a Glycine max pale green leaf 3-1 (Gmpgl3-1) mutant was isolated from the soybean mutagenized population. The Gmpgl3-1 mutant presented with decreased chlorophyll contents, reduced chloroplast stroma thylakoids, reduced yields, and decreased numbers of pods per plant. Bulked segregant analysis (BSA) together with map-based cloning revealed a single-nucleotide non-synonymous mutation at the 341st nucleotide of the first exon of the chloroplast development-related GmTic110a gene. The phenotype of the knockout plants was the same as that of the mutant. The GmTic110a gene was highly expressed in the leaves at various developmental stages, and its protein was localized to the inner chloroplast membrane. Split luciferase complementation assays and coimmunoprecipitation (co-IP) experiments revealed that GmTic110a interacted with GmTic20, GmTic40a, and GmTic40b in tobacco leaves. These results indicated that the GmTic110a gene plays an important role in chloroplast development.https://www.frontiersin.org/articles/10.3389/fpls.2022.892077/fullsoybeanGmpgl3 mutantgene mappingGmTic110aGmTic20GmTic40a/b |
| spellingShingle | Hui Yu Qiushi Wang Zhirui Zhang Zhirui Zhang Tao Wu Tao Wu Xinjing Yang Xinjing Yang Xiaobin Zhu Xiaobin Zhu Yongheng Ye Yongheng Ye Jiantian Leng Suxin Yang Xianzhong Feng Xianzhong Feng Xianzhong Feng Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast Development Frontiers in Plant Science soybean Gmpgl3 mutant gene mapping GmTic110a GmTic20 GmTic40a/b |
| title | Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast Development |
| title_full | Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast Development |
| title_fullStr | Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast Development |
| title_full_unstemmed | Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast Development |
| title_short | Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast Development |
| title_sort | genetic mapping of the gmpgl3 mutant reveals the function of gmtic110a in soybean chloroplast development |
| topic | soybean Gmpgl3 mutant gene mapping GmTic110a GmTic20 GmTic40a/b |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.892077/full |
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