Conserved Inhibition of Neutrophil Extracellular Trap Release by Clinical Candida albicans Biofilms
Candida albicans biofilms are difficult to eradicate due to their resistance to host defenses and antifungal drugs. Although neutrophils are the primary responder to C. albicans during invasive candidiasis, biofilms resist killing by neutrophils. Prior investigation, with the commonly used laborator...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-09-01
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| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/3/3/49 |
| _version_ | 1818495059586187264 |
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| author | John F. Kernien Chad J. Johnson Jeniel E. Nett |
| author_facet | John F. Kernien Chad J. Johnson Jeniel E. Nett |
| author_sort | John F. Kernien |
| collection | DOAJ |
| description | Candida albicans biofilms are difficult to eradicate due to their resistance to host defenses and antifungal drugs. Although neutrophils are the primary responder to C. albicans during invasive candidiasis, biofilms resist killing by neutrophils. Prior investigation, with the commonly used laboratory strain SC5314, linked this phenotype to the impaired release of neutrophil extracellular traps (NETs), which are structures of DNA, histones, and antimicrobial proteins involved in extracellular microbial killing. Considering the diversity of C. albicans biofilms, we examined the neutrophil response to a subset of clinical isolates forming biofilms with varying depths and architectures. Using fluorescent staining of DNA and scanning electron microscopy, we found that inhibition of NET release was conserved across the clinical isolates. However, the dampening of the production of reactive oxygen species (ROS) by neutrophils was strain-dependent, suggesting an uncoupling of ROS and NET inhibition. Our findings show that biofilms formed by clinical C. albicans isolates uniformly impair the release of NETs. Further investigation of this pathway may reveal novel approaches to augment immunity to C. albicans biofilm infections. |
| first_indexed | 2024-12-10T18:15:13Z |
| format | Article |
| id | doaj.art-5cc93ebe4e314315ab1e578771382e65 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-12-10T18:15:13Z |
| publishDate | 2017-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-5cc93ebe4e314315ab1e578771382e652022-12-22T01:38:21ZengMDPI AGJournal of Fungi2309-608X2017-09-01334910.3390/jof3030049jof3030049Conserved Inhibition of Neutrophil Extracellular Trap Release by Clinical Candida albicans BiofilmsJohn F. Kernien0Chad J. Johnson1Jeniel E. Nett2Department of Medicine, University of Wisconsin, Madison, WI 53706-1521, USADepartment of Medicine, University of Wisconsin, Madison, WI 53706-1521, USADepartment of Medicine, University of Wisconsin, Madison, WI 53706-1521, USACandida albicans biofilms are difficult to eradicate due to their resistance to host defenses and antifungal drugs. Although neutrophils are the primary responder to C. albicans during invasive candidiasis, biofilms resist killing by neutrophils. Prior investigation, with the commonly used laboratory strain SC5314, linked this phenotype to the impaired release of neutrophil extracellular traps (NETs), which are structures of DNA, histones, and antimicrobial proteins involved in extracellular microbial killing. Considering the diversity of C. albicans biofilms, we examined the neutrophil response to a subset of clinical isolates forming biofilms with varying depths and architectures. Using fluorescent staining of DNA and scanning electron microscopy, we found that inhibition of NET release was conserved across the clinical isolates. However, the dampening of the production of reactive oxygen species (ROS) by neutrophils was strain-dependent, suggesting an uncoupling of ROS and NET inhibition. Our findings show that biofilms formed by clinical C. albicans isolates uniformly impair the release of NETs. Further investigation of this pathway may reveal novel approaches to augment immunity to C. albicans biofilm infections.https://www.mdpi.com/2309-608X/3/3/49Candidabiofilmneutrophilneutrophil extracellular trapfungalreactive oxygen speciesfilamentationphagocyte |
| spellingShingle | John F. Kernien Chad J. Johnson Jeniel E. Nett Conserved Inhibition of Neutrophil Extracellular Trap Release by Clinical Candida albicans Biofilms Journal of Fungi Candida biofilm neutrophil neutrophil extracellular trap fungal reactive oxygen species filamentation phagocyte |
| title | Conserved Inhibition of Neutrophil Extracellular Trap Release by Clinical Candida albicans Biofilms |
| title_full | Conserved Inhibition of Neutrophil Extracellular Trap Release by Clinical Candida albicans Biofilms |
| title_fullStr | Conserved Inhibition of Neutrophil Extracellular Trap Release by Clinical Candida albicans Biofilms |
| title_full_unstemmed | Conserved Inhibition of Neutrophil Extracellular Trap Release by Clinical Candida albicans Biofilms |
| title_short | Conserved Inhibition of Neutrophil Extracellular Trap Release by Clinical Candida albicans Biofilms |
| title_sort | conserved inhibition of neutrophil extracellular trap release by clinical candida albicans biofilms |
| topic | Candida biofilm neutrophil neutrophil extracellular trap fungal reactive oxygen species filamentation phagocyte |
| url | https://www.mdpi.com/2309-608X/3/3/49 |
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