Synthesis and anti-intracellular Copper overload evaluation of Nanoconjugated D-penicillamine –Dendrimer in Wilson’s model cells
Background and Objective: Wilson’s disease (WD) is caused by mutation to the cooer-transporting gene ATP7B. Chelation therapy is the main protochol of treatment for patients with Wilson’s disease. D-penicillamine is one of the well-known chelator agants which is used in WD treatment but it can not e...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | fas |
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Golestan University of Medical Sciences
2016-03-01
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| Series: | مجله دانشگاه علوم پزشکی گرگان |
| Subjects: | |
| Online Access: | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-876&slc_lang=en&sid=1 |
| _version_ | 1818114022652772352 |
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| author | Fani N Shafiee Ardestani M Yaghmaei P Assadi A Barzegar Behrouz A |
| author_facet | Fani N Shafiee Ardestani M Yaghmaei P Assadi A Barzegar Behrouz A |
| author_sort | Fani N |
| collection | DOAJ |
| description | Background and Objective: Wilson’s disease (WD) is caused by mutation to the cooer-transporting gene ATP7B. Chelation therapy is the main protochol of treatment for patients with Wilson’s disease. D-penicillamine is one of the well-known chelator agants which is used in WD treatment but it can not enter into the intracellular space.This study was done to evaluate the synthesis and anti-intracellular Copper overload evaluation of Nanoconjugated D-penicillamine –Dendrimer in Wilson’s model cells. Methods: In this descriptive-analytic study, initially 0.01 mm polyethylene glycol (PEG) and 0.0018 mm citric acid, Dendrimer was synthesized. After purification by dialysis bag and lyophilization, 10mg dendrimer was conjugated to 3.3mg D-penicillamine. Nanoconjugated D-penicillamine-dendrimer was injected on Wilson’s model cells. After incubation and centrifugation intracellular measurement of copper concentration and FTIR test were done. Results: Copper accumulation significantly reduced in the HepG2 WD cell by Nanoconjugated D-penicillamine - Dendrimer in compared to D-penicillamine (P<0.05). Copper accumulation was determined to be 46.61. MTT assay showed no toxicological damage in HepG2 WD cell. Conclusion: Nanoconjugated D-penicillamine –Dendrimer can reduces intracellular concentration of Copper. |
| first_indexed | 2024-12-11T03:44:07Z |
| format | Article |
| id | doaj.art-5cc9c7f13a4a42868c8b597018de2cfd |
| institution | Directory Open Access Journal |
| issn | 1562-4765 2008-4080 |
| language | fas |
| last_indexed | 2024-12-11T03:44:07Z |
| publishDate | 2016-03-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی گرگان |
| spelling | doaj.art-5cc9c7f13a4a42868c8b597018de2cfd2022-12-22T01:22:04ZfasGolestan University of Medical Sciencesمجله دانشگاه علوم پزشکی گرگان1562-47652008-40802016-03-01181114119Synthesis and anti-intracellular Copper overload evaluation of Nanoconjugated D-penicillamine –Dendrimer in Wilson’s model cellsFani N0Shafiee Ardestani M1Yaghmaei P2Assadi A3Barzegar Behrouz A4 Department of Biochemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran Assistant Professor, Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Associate Professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Department of Biochemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran M.Sc in Physiology, Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Background and Objective: Wilson’s disease (WD) is caused by mutation to the cooer-transporting gene ATP7B. Chelation therapy is the main protochol of treatment for patients with Wilson’s disease. D-penicillamine is one of the well-known chelator agants which is used in WD treatment but it can not enter into the intracellular space.This study was done to evaluate the synthesis and anti-intracellular Copper overload evaluation of Nanoconjugated D-penicillamine –Dendrimer in Wilson’s model cells. Methods: In this descriptive-analytic study, initially 0.01 mm polyethylene glycol (PEG) and 0.0018 mm citric acid, Dendrimer was synthesized. After purification by dialysis bag and lyophilization, 10mg dendrimer was conjugated to 3.3mg D-penicillamine. Nanoconjugated D-penicillamine-dendrimer was injected on Wilson’s model cells. After incubation and centrifugation intracellular measurement of copper concentration and FTIR test were done. Results: Copper accumulation significantly reduced in the HepG2 WD cell by Nanoconjugated D-penicillamine - Dendrimer in compared to D-penicillamine (P<0.05). Copper accumulation was determined to be 46.61. MTT assay showed no toxicological damage in HepG2 WD cell. Conclusion: Nanoconjugated D-penicillamine –Dendrimer can reduces intracellular concentration of Copper.http://goums.ac.ir/journal/browse.php?a_code=A-10-1-876&slc_lang=en&sid=1Wilson’s DiseaseNanoconjugated D-penicillamine-dendrimer |
| spellingShingle | Fani N Shafiee Ardestani M Yaghmaei P Assadi A Barzegar Behrouz A Synthesis and anti-intracellular Copper overload evaluation of Nanoconjugated D-penicillamine –Dendrimer in Wilson’s model cells مجله دانشگاه علوم پزشکی گرگان Wilson’s Disease Nanoconjugated D-penicillamine-dendrimer |
| title | Synthesis and anti-intracellular Copper overload evaluation of Nanoconjugated D-penicillamine –Dendrimer in Wilson’s model cells |
| title_full | Synthesis and anti-intracellular Copper overload evaluation of Nanoconjugated D-penicillamine –Dendrimer in Wilson’s model cells |
| title_fullStr | Synthesis and anti-intracellular Copper overload evaluation of Nanoconjugated D-penicillamine –Dendrimer in Wilson’s model cells |
| title_full_unstemmed | Synthesis and anti-intracellular Copper overload evaluation of Nanoconjugated D-penicillamine –Dendrimer in Wilson’s model cells |
| title_short | Synthesis and anti-intracellular Copper overload evaluation of Nanoconjugated D-penicillamine –Dendrimer in Wilson’s model cells |
| title_sort | synthesis and anti intracellular copper overload evaluation of nanoconjugated d penicillamine dendrimer in wilson s model cells |
| topic | Wilson’s Disease Nanoconjugated D-penicillamine-dendrimer |
| url | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-876&slc_lang=en&sid=1 |
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