Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Scedosporium, Acremonium-Like, Scopulariopsis, and Microascus Species
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a powerful microorganism identification tool. Research on MALDI-TOF MS identification of rare filamentous fungi is still lacking. This study aimed to evaluate the performance of MALDI-TOF MS in...
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.841286/full |
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| author | Linwei Wei Linwei Wei Linwei Wei Linwei Wei Jin Shao Jin Shao Jin Shao Jin Shao Yinggai Song Yinggai Song Yinggai Song Yinggai Song Zhe Wan Zhe Wan Zhe Wan Zhe Wan Limin Yao Limin Yao Limin Yao Limin Yao Hong Wang Hong Wang Hong Wang Hong Wang Jin Yu Jin Yu Jin Yu Jin Yu |
| author_facet | Linwei Wei Linwei Wei Linwei Wei Linwei Wei Jin Shao Jin Shao Jin Shao Jin Shao Yinggai Song Yinggai Song Yinggai Song Yinggai Song Zhe Wan Zhe Wan Zhe Wan Zhe Wan Limin Yao Limin Yao Limin Yao Limin Yao Hong Wang Hong Wang Hong Wang Hong Wang Jin Yu Jin Yu Jin Yu Jin Yu |
| author_sort | Linwei Wei |
| collection | DOAJ |
| description | Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a powerful microorganism identification tool. Research on MALDI-TOF MS identification of rare filamentous fungi is still lacking. This study aimed to evaluate the performance of MALDI-TOF MS in the identification of Scedosporium, Acremonium-like, Scopulariopsis, and Microascus species. Sabouraud broth cultivation and formic acid/acetonitrile protein extraction were used for MALDI-TOF MS identification by a Bruker Biotyper system. An in-house database containing 29 isolates of Scedosporium, Acremonium-like, Scopulariopsis, and Microascus spp. was constructed. A total of 52 clinical isolates were identified using the Bruker Filamentous Fungi Library v1.0 (FFL v1.0) alone, and Filamentous Fungi Library v1.0 plus the in-house library, respectively. The mass spectrum profile (MSP) dendrograms of the 28 Scedosporium isolates, 26 Acremonium-like isolates, and 27 Scopulariopsis and Microascus isolates were constructed by MALDI Biotyper OC 4.0 software, respectively. The correct species identification rate significantly improved when using the combined databases compared with that when using FFL v1.0 alone (Scedosporium spp., 75% versus 0%; Acremonium-like spp., 100% versus 0%; Scopulariopsis and Microascus spp., 100% versus 62.5%). The MSP dendrograms differentiated Acremonium-like species, Scopulariopsis and Microascus species clearly, but cannot distinguish species in the Scedosporium apiospermum complex. In conclusion, with an expanded database, MALDI-TOF MS is an effective tool for the identification of Scedosporium, Acremonium-like, Scopulariopsis, and Microascus species. |
| first_indexed | 2024-12-22T03:27:52Z |
| format | Article |
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| language | English |
| last_indexed | 2024-12-22T03:27:52Z |
| publishDate | 2022-03-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-5ccb64e20be740309eeeb351a7fd92e02022-12-21T18:40:33ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-03-011310.3389/fmicb.2022.841286841286Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Scedosporium, Acremonium-Like, Scopulariopsis, and Microascus SpeciesLinwei Wei0Linwei Wei1Linwei Wei2Linwei Wei3Jin Shao4Jin Shao5Jin Shao6Jin Shao7Yinggai Song8Yinggai Song9Yinggai Song10Yinggai Song11Zhe Wan12Zhe Wan13Zhe Wan14Zhe Wan15Limin Yao16Limin Yao17Limin Yao18Limin Yao19Hong Wang20Hong Wang21Hong Wang22Hong Wang23Jin Yu24Jin Yu25Jin Yu26Jin Yu27Department of Dermatology and Venereology, Peking University First Hospital, Beijing, ChinaResearch Center for Medical Mycology, Peking University, Beijing, ChinaNational Clinical Research Center for Skin and Immune Diseases, Beijing, ChinaBeijing Key Laboratory of Molecular Diagnosis on Dermatoses, Beijing, ChinaDepartment of Dermatology and Venereology, Peking University First Hospital, Beijing, ChinaResearch Center for Medical Mycology, Peking University, Beijing, ChinaNational Clinical Research Center for Skin and Immune Diseases, Beijing, ChinaBeijing Key Laboratory of Molecular Diagnosis on Dermatoses, Beijing, ChinaDepartment of Dermatology and Venereology, Peking University First Hospital, Beijing, ChinaResearch Center for Medical Mycology, Peking University, Beijing, ChinaNational Clinical Research Center for Skin and Immune Diseases, Beijing, ChinaBeijing Key Laboratory of Molecular Diagnosis on Dermatoses, Beijing, ChinaDepartment of Dermatology and Venereology, Peking University First Hospital, Beijing, ChinaResearch Center for Medical Mycology, Peking University, Beijing, ChinaNational Clinical Research Center for Skin and Immune Diseases, Beijing, ChinaBeijing Key Laboratory of Molecular Diagnosis on Dermatoses, Beijing, ChinaDepartment of Dermatology and Venereology, Peking University First Hospital, Beijing, ChinaResearch Center for Medical Mycology, Peking University, Beijing, ChinaNational Clinical Research Center for Skin and Immune Diseases, Beijing, ChinaBeijing Key Laboratory of Molecular Diagnosis on Dermatoses, Beijing, ChinaDepartment of Dermatology and Venereology, Peking University First Hospital, Beijing, ChinaResearch Center for Medical Mycology, Peking University, Beijing, ChinaNational Clinical Research Center for Skin and Immune Diseases, Beijing, ChinaBeijing Key Laboratory of Molecular Diagnosis on Dermatoses, Beijing, ChinaDepartment of Dermatology and Venereology, Peking University First Hospital, Beijing, ChinaResearch Center for Medical Mycology, Peking University, Beijing, ChinaNational Clinical Research Center for Skin and Immune Diseases, Beijing, ChinaBeijing Key Laboratory of Molecular Diagnosis on Dermatoses, Beijing, ChinaMatrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a powerful microorganism identification tool. Research on MALDI-TOF MS identification of rare filamentous fungi is still lacking. This study aimed to evaluate the performance of MALDI-TOF MS in the identification of Scedosporium, Acremonium-like, Scopulariopsis, and Microascus species. Sabouraud broth cultivation and formic acid/acetonitrile protein extraction were used for MALDI-TOF MS identification by a Bruker Biotyper system. An in-house database containing 29 isolates of Scedosporium, Acremonium-like, Scopulariopsis, and Microascus spp. was constructed. A total of 52 clinical isolates were identified using the Bruker Filamentous Fungi Library v1.0 (FFL v1.0) alone, and Filamentous Fungi Library v1.0 plus the in-house library, respectively. The mass spectrum profile (MSP) dendrograms of the 28 Scedosporium isolates, 26 Acremonium-like isolates, and 27 Scopulariopsis and Microascus isolates were constructed by MALDI Biotyper OC 4.0 software, respectively. The correct species identification rate significantly improved when using the combined databases compared with that when using FFL v1.0 alone (Scedosporium spp., 75% versus 0%; Acremonium-like spp., 100% versus 0%; Scopulariopsis and Microascus spp., 100% versus 62.5%). The MSP dendrograms differentiated Acremonium-like species, Scopulariopsis and Microascus species clearly, but cannot distinguish species in the Scedosporium apiospermum complex. In conclusion, with an expanded database, MALDI-TOF MS is an effective tool for the identification of Scedosporium, Acremonium-like, Scopulariopsis, and Microascus species.https://www.frontiersin.org/articles/10.3389/fmicb.2022.841286/fullMALDI-TOS MSidentificationScedosporiumAcremoniumScopulariopsisMicroascus |
| spellingShingle | Linwei Wei Linwei Wei Linwei Wei Linwei Wei Jin Shao Jin Shao Jin Shao Jin Shao Yinggai Song Yinggai Song Yinggai Song Yinggai Song Zhe Wan Zhe Wan Zhe Wan Zhe Wan Limin Yao Limin Yao Limin Yao Limin Yao Hong Wang Hong Wang Hong Wang Hong Wang Jin Yu Jin Yu Jin Yu Jin Yu Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Scedosporium, Acremonium-Like, Scopulariopsis, and Microascus Species Frontiers in Microbiology MALDI-TOS MS identification Scedosporium Acremonium Scopulariopsis Microascus |
| title | Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Scedosporium, Acremonium-Like, Scopulariopsis, and Microascus Species |
| title_full | Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Scedosporium, Acremonium-Like, Scopulariopsis, and Microascus Species |
| title_fullStr | Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Scedosporium, Acremonium-Like, Scopulariopsis, and Microascus Species |
| title_full_unstemmed | Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Scedosporium, Acremonium-Like, Scopulariopsis, and Microascus Species |
| title_short | Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Scedosporium, Acremonium-Like, Scopulariopsis, and Microascus Species |
| title_sort | performance of matrix assisted laser desorption ionization time of flight mass spectrometry for identification of scedosporium acremonium like scopulariopsis and microascus species |
| topic | MALDI-TOS MS identification Scedosporium Acremonium Scopulariopsis Microascus |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.841286/full |
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