Identification of QTLs associated with curd architecture in cauliflower
Abstract Background Curd architecture is one of the most important characters determining the curd morphology of cauliflower. However, the genetic mechanism dissection of this complex trait at molecular level is lacking. Genes/QTLs responsible for the morphological differences between present-day lo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-04-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-020-02377-5 |
| _version_ | 1818962227300925440 |
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| author | Zhen-Qing Zhao Xiao-Guang Sheng Hui-Fang Yu Jian-Sheng Wang Yu-Sen Shen Hong-Hui Gu |
| author_facet | Zhen-Qing Zhao Xiao-Guang Sheng Hui-Fang Yu Jian-Sheng Wang Yu-Sen Shen Hong-Hui Gu |
| author_sort | Zhen-Qing Zhao |
| collection | DOAJ |
| description | Abstract Background Curd architecture is one of the most important characters determining the curd morphology of cauliflower. However, the genetic mechanism dissection of this complex trait at molecular level is lacking. Genes/QTLs responsible for the morphological differences between present-day loose-curd and compact-curd cauliflower haven’t been well revealed. Results Herein, by using a common compact-curd parent and two loose-curd parents, we developed two double haploid (DH) populations including 122 and 79 lines, respectively. For each population, we decomposed the curd architecture concept into four parameters (basal diameter, stalk length, stalk angle and curd solidity), and collected corresponding phenotypic data for each parameter across two environments. The Kosambi function and composite interval mapping algorithm were conducted to construct the linkage map and analyze the QTLs associated with curd architecture parameters. A total of 20 QTLs were detected with the minimum likelihood of odd (LOD) values ranging from 2.61 to 8.38 and the percentage of the phenotypic variance explained by each QTL (PVE) varying between 7.69 and 25.10%. Of these, two QTLs controlling stalk length (qSL.C6–1, qSL.C6–2) and two QTLs controlling curd solidity (qCS.C6–1 and qCS.C6–2) were steadily expressed in both environments. Further, qSL.C6–1, qSL.C6–2, qCS.C6–1 and qCS.C6–4 fell into the same chromosomal region of the reference genome, indicating that these loci are involved in pleiotropic effects or are tightly linked. Conclusion The current study identified a series of QTLs associated with curd architecture parameters, which might contribute essentially to the formation of present-day loose-curd cauliflower that is widely cultivated in China. These results may pave the way for intensive deciphering the molecular mechanisms of curd development and for marker-assisted selection of curd morphology in cauliflower breeding. |
| first_indexed | 2024-12-20T12:25:58Z |
| format | Article |
| id | doaj.art-5ccc2077793d4d95a4cbd48b1f880d0a |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-20T12:25:58Z |
| publishDate | 2020-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-5ccc2077793d4d95a4cbd48b1f880d0a2022-12-21T19:40:53ZengBMCBMC Plant Biology1471-22292020-04-012011810.1186/s12870-020-02377-5Identification of QTLs associated with curd architecture in cauliflowerZhen-Qing Zhao0Xiao-Guang Sheng1Hui-Fang Yu2Jian-Sheng Wang3Yu-Sen Shen4Hong-Hui Gu5Institute of Vegetables, Zhejiang Academy of Agricultural SciencesInstitute of Vegetables, Zhejiang Academy of Agricultural SciencesInstitute of Vegetables, Zhejiang Academy of Agricultural SciencesInstitute of Vegetables, Zhejiang Academy of Agricultural SciencesInstitute of Vegetables, Zhejiang Academy of Agricultural SciencesInstitute of Vegetables, Zhejiang Academy of Agricultural SciencesAbstract Background Curd architecture is one of the most important characters determining the curd morphology of cauliflower. However, the genetic mechanism dissection of this complex trait at molecular level is lacking. Genes/QTLs responsible for the morphological differences between present-day loose-curd and compact-curd cauliflower haven’t been well revealed. Results Herein, by using a common compact-curd parent and two loose-curd parents, we developed two double haploid (DH) populations including 122 and 79 lines, respectively. For each population, we decomposed the curd architecture concept into four parameters (basal diameter, stalk length, stalk angle and curd solidity), and collected corresponding phenotypic data for each parameter across two environments. The Kosambi function and composite interval mapping algorithm were conducted to construct the linkage map and analyze the QTLs associated with curd architecture parameters. A total of 20 QTLs were detected with the minimum likelihood of odd (LOD) values ranging from 2.61 to 8.38 and the percentage of the phenotypic variance explained by each QTL (PVE) varying between 7.69 and 25.10%. Of these, two QTLs controlling stalk length (qSL.C6–1, qSL.C6–2) and two QTLs controlling curd solidity (qCS.C6–1 and qCS.C6–2) were steadily expressed in both environments. Further, qSL.C6–1, qSL.C6–2, qCS.C6–1 and qCS.C6–4 fell into the same chromosomal region of the reference genome, indicating that these loci are involved in pleiotropic effects or are tightly linked. Conclusion The current study identified a series of QTLs associated with curd architecture parameters, which might contribute essentially to the formation of present-day loose-curd cauliflower that is widely cultivated in China. These results may pave the way for intensive deciphering the molecular mechanisms of curd development and for marker-assisted selection of curd morphology in cauliflower breeding.http://link.springer.com/article/10.1186/s12870-020-02377-5CauliflowerCurd architectureLinkage analysisGenetic mapQTL mapping |
| spellingShingle | Zhen-Qing Zhao Xiao-Guang Sheng Hui-Fang Yu Jian-Sheng Wang Yu-Sen Shen Hong-Hui Gu Identification of QTLs associated with curd architecture in cauliflower BMC Plant Biology Cauliflower Curd architecture Linkage analysis Genetic map QTL mapping |
| title | Identification of QTLs associated with curd architecture in cauliflower |
| title_full | Identification of QTLs associated with curd architecture in cauliflower |
| title_fullStr | Identification of QTLs associated with curd architecture in cauliflower |
| title_full_unstemmed | Identification of QTLs associated with curd architecture in cauliflower |
| title_short | Identification of QTLs associated with curd architecture in cauliflower |
| title_sort | identification of qtls associated with curd architecture in cauliflower |
| topic | Cauliflower Curd architecture Linkage analysis Genetic map QTL mapping |
| url | http://link.springer.com/article/10.1186/s12870-020-02377-5 |
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