Plant Response to Cold Stress: Cold Stress Changes Antioxidant Metabolism in Heading Type Kimchi Cabbage (<i>Brassica rapa</i> L. ssp. Pekinensis)
Cold stress is known as the important yield-limiting factor of heading type Kimchi cabbage (HtKc, <i>Brassica rapa</i> L. ssp. pekinensis), which is an economically important crop worldwide. However, the biochemical and molecular responses to cold stress in HtKc are largely unknown. In t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/4/700 |
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| author | Seung Hee Eom Min-A Ahn Eunhui Kim Hee Ju Lee Jin Hyoung Lee Seung Hwan Wi Sung Kyeom Kim Heung Bin Lim Tae Kyung Hyun |
| author_facet | Seung Hee Eom Min-A Ahn Eunhui Kim Hee Ju Lee Jin Hyoung Lee Seung Hwan Wi Sung Kyeom Kim Heung Bin Lim Tae Kyung Hyun |
| author_sort | Seung Hee Eom |
| collection | DOAJ |
| description | Cold stress is known as the important yield-limiting factor of heading type Kimchi cabbage (HtKc, <i>Brassica rapa</i> L. ssp. pekinensis), which is an economically important crop worldwide. However, the biochemical and molecular responses to cold stress in HtKc are largely unknown. In this study, we conducted transcriptome analyses on HtKc grown under normal versus cold conditions to investigate the molecular mechanism underlying HtKc responses to cold stress. A total of 2131 genes (936 up-regulated and 1195 down-regulated) were identified as differentially expressed genes and were significantly annotated in the category of “response to stimulus”. In addition, cold stress caused the accumulation of polyphenolic compounds, including p-coumaric, ferulic, and sinapic acids, in HtKc by inducing the phenylpropanoid pathway. The results of the chemical-based antioxidant assay indicated that the cold-induced polyphenolic compounds improved the free-radical scavenging activity and antioxidant capacity, suggesting that the phenylpropanoid pathway induced by cold stress contributes to resistance to cold-induced reactive oxygen species in HtKc. Taken together, our results will serve as an important base to improve the cold tolerance in plants via enhancing the antioxidant machinery. |
| first_indexed | 2024-03-09T11:13:55Z |
| format | Article |
| id | doaj.art-5ccd855ce7b84b158fcd84e306113ead |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T11:13:55Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-5ccd855ce7b84b158fcd84e306113ead2023-12-01T00:35:21ZengMDPI AGAntioxidants2076-39212022-04-0111470010.3390/antiox11040700Plant Response to Cold Stress: Cold Stress Changes Antioxidant Metabolism in Heading Type Kimchi Cabbage (<i>Brassica rapa</i> L. ssp. Pekinensis)Seung Hee Eom0Min-A Ahn1Eunhui Kim2Hee Ju Lee3Jin Hyoung Lee4Seung Hwan Wi5Sung Kyeom Kim6Heung Bin Lim7Tae Kyung Hyun8Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University, Cheongju 28644, KoreaDepartment of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University, Cheongju 28644, KoreaDepartment of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University, Cheongju 28644, KoreaVegetable Research Division, National Institute of Horticultural & Herbal Science, Wanju 55365, KoreaVegetable Research Division, National Institute of Horticultural & Herbal Science, Wanju 55365, KoreaVegetable Research Division, National Institute of Horticultural & Herbal Science, Wanju 55365, KoreaDepartment of Horticultural Science, College of Agriculture and Life Science, Kyungpook National University, Daegu 41566, KoreaDepartment of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University, Cheongju 28644, KoreaDepartment of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University, Cheongju 28644, KoreaCold stress is known as the important yield-limiting factor of heading type Kimchi cabbage (HtKc, <i>Brassica rapa</i> L. ssp. pekinensis), which is an economically important crop worldwide. However, the biochemical and molecular responses to cold stress in HtKc are largely unknown. In this study, we conducted transcriptome analyses on HtKc grown under normal versus cold conditions to investigate the molecular mechanism underlying HtKc responses to cold stress. A total of 2131 genes (936 up-regulated and 1195 down-regulated) were identified as differentially expressed genes and were significantly annotated in the category of “response to stimulus”. In addition, cold stress caused the accumulation of polyphenolic compounds, including p-coumaric, ferulic, and sinapic acids, in HtKc by inducing the phenylpropanoid pathway. The results of the chemical-based antioxidant assay indicated that the cold-induced polyphenolic compounds improved the free-radical scavenging activity and antioxidant capacity, suggesting that the phenylpropanoid pathway induced by cold stress contributes to resistance to cold-induced reactive oxygen species in HtKc. Taken together, our results will serve as an important base to improve the cold tolerance in plants via enhancing the antioxidant machinery.https://www.mdpi.com/2076-3921/11/4/700antioxidantkimchi cabbagecold stressphenylpropanoid pathway |
| spellingShingle | Seung Hee Eom Min-A Ahn Eunhui Kim Hee Ju Lee Jin Hyoung Lee Seung Hwan Wi Sung Kyeom Kim Heung Bin Lim Tae Kyung Hyun Plant Response to Cold Stress: Cold Stress Changes Antioxidant Metabolism in Heading Type Kimchi Cabbage (<i>Brassica rapa</i> L. ssp. Pekinensis) Antioxidants antioxidant kimchi cabbage cold stress phenylpropanoid pathway |
| title | Plant Response to Cold Stress: Cold Stress Changes Antioxidant Metabolism in Heading Type Kimchi Cabbage (<i>Brassica rapa</i> L. ssp. Pekinensis) |
| title_full | Plant Response to Cold Stress: Cold Stress Changes Antioxidant Metabolism in Heading Type Kimchi Cabbage (<i>Brassica rapa</i> L. ssp. Pekinensis) |
| title_fullStr | Plant Response to Cold Stress: Cold Stress Changes Antioxidant Metabolism in Heading Type Kimchi Cabbage (<i>Brassica rapa</i> L. ssp. Pekinensis) |
| title_full_unstemmed | Plant Response to Cold Stress: Cold Stress Changes Antioxidant Metabolism in Heading Type Kimchi Cabbage (<i>Brassica rapa</i> L. ssp. Pekinensis) |
| title_short | Plant Response to Cold Stress: Cold Stress Changes Antioxidant Metabolism in Heading Type Kimchi Cabbage (<i>Brassica rapa</i> L. ssp. Pekinensis) |
| title_sort | plant response to cold stress cold stress changes antioxidant metabolism in heading type kimchi cabbage i brassica rapa i l ssp pekinensis |
| topic | antioxidant kimchi cabbage cold stress phenylpropanoid pathway |
| url | https://www.mdpi.com/2076-3921/11/4/700 |
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