Response of Nitrate Metabolism in Seedlings of Oilseed Rape (Brassica napus L.) to Low Oxygen Stress
In order to understand the response of nitrate metabolism in seedlings of oilseed rape (Brassica napus L.) to low oxygen stress (LOS), two cultivars were studied at different light, LOS time and exogenous nitrate concentrations under hydroponic stress. Results show that N-uptake and dry matter of ra...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-11-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311913606930 |
| _version_ | 1819000459054022656 |
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| author | Chang-bing YU Yu-yun XIE Jia-jia HOU You-qiang FU Hong SHEN Xing LIAO |
| author_facet | Chang-bing YU Yu-yun XIE Jia-jia HOU You-qiang FU Hong SHEN Xing LIAO |
| author_sort | Chang-bing YU |
| collection | DOAJ |
| description | In order to understand the response of nitrate metabolism in seedlings of oilseed rape (Brassica napus L.) to low oxygen stress (LOS), two cultivars were studied at different light, LOS time and exogenous nitrate concentrations under hydroponic stress. Results show that N-uptake and dry matter of rape seedlings were decreased after LOS stress while nitrate accumulation (NA) under LOS was induced by darkness. Nitrate accumulation peaked at 3 d while root activity (RA, defined as dehydrogenase activity) decreased with prolonged waterlogging exposure. Exogenous nitrate significantly elevated NA and RA. Tungstate (TS) and LOS inhibited nitrate reductase (NR) activity while NR transcription and activity were enhanced by exogenous nitrate. Low oxygen stress stimulated the activity of superoxide dismutase (SOD) and peroxidase (POD) slightly, but inhibited that of catalase (CAT). B. napus L. Zhongshuang 10 (ZS10), a LOS tolerant cultivar, displayed smaller decrease upon dry matter under LOS, higher NA in darkness and lower NA in light than B. napus L. Ganlan CC (GAC), a LOS sensitive variety. ZS10 had lower NA and higher RA after waterlogging and exogenous nitrate treatment, and higher NR activity under TS inhibition than GAC, but the activity of antioxidant enzymes did not change under LOS. The results indicate that nitrate metabolism involved tolerance of rape seedlings to LOS, with lower accumulation and higher reduction of nitrate being related to higher LOS tolerance of rape seedlings exposed to waterlogging. |
| first_indexed | 2024-12-20T22:33:39Z |
| format | Article |
| id | doaj.art-5ccf30c6e6584a949078a4a8d46b06db |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-20T22:33:39Z |
| publishDate | 2014-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-5ccf30c6e6584a949078a4a8d46b06db2022-12-21T19:24:40ZengElsevierJournal of Integrative Agriculture2095-31192014-11-01131124162423Response of Nitrate Metabolism in Seedlings of Oilseed Rape (Brassica napus L.) to Low Oxygen StressChang-bing YU0Yu-yun XIE1Jia-jia HOU2You-qiang FU3Hong SHEN4Xing LIAO5Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture/Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, P.R. China; YU Chang-bing, Tel: +86-27-86712256College of Resources and Environment, South China Agricultural University, Guangzhou 510642, P.R. ChinaCollege of Resources and Environment, South China Agricultural University, Guangzhou 510642, P.R. ChinaCollege of Resources and Environment, South China Agricultural University, Guangzhou 510642, P.R. ChinaCollege of Resources and Environment, South China Agricultural University, Guangzhou 510642, P.R. China; Correspondence SHEN Hong, Tel: +86-20-85281822Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture/Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, P.R. China; LIAO Xing, Tel: +86-27-86712256In order to understand the response of nitrate metabolism in seedlings of oilseed rape (Brassica napus L.) to low oxygen stress (LOS), two cultivars were studied at different light, LOS time and exogenous nitrate concentrations under hydroponic stress. Results show that N-uptake and dry matter of rape seedlings were decreased after LOS stress while nitrate accumulation (NA) under LOS was induced by darkness. Nitrate accumulation peaked at 3 d while root activity (RA, defined as dehydrogenase activity) decreased with prolonged waterlogging exposure. Exogenous nitrate significantly elevated NA and RA. Tungstate (TS) and LOS inhibited nitrate reductase (NR) activity while NR transcription and activity were enhanced by exogenous nitrate. Low oxygen stress stimulated the activity of superoxide dismutase (SOD) and peroxidase (POD) slightly, but inhibited that of catalase (CAT). B. napus L. Zhongshuang 10 (ZS10), a LOS tolerant cultivar, displayed smaller decrease upon dry matter under LOS, higher NA in darkness and lower NA in light than B. napus L. Ganlan CC (GAC), a LOS sensitive variety. ZS10 had lower NA and higher RA after waterlogging and exogenous nitrate treatment, and higher NR activity under TS inhibition than GAC, but the activity of antioxidant enzymes did not change under LOS. The results indicate that nitrate metabolism involved tolerance of rape seedlings to LOS, with lower accumulation and higher reduction of nitrate being related to higher LOS tolerance of rape seedlings exposed to waterlogging.http://www.sciencedirect.com/science/article/pii/S2095311913606930Brassica napuslow oxygen stressnitrate accumulationnitrate reductasegene expression |
| spellingShingle | Chang-bing YU Yu-yun XIE Jia-jia HOU You-qiang FU Hong SHEN Xing LIAO Response of Nitrate Metabolism in Seedlings of Oilseed Rape (Brassica napus L.) to Low Oxygen Stress Journal of Integrative Agriculture Brassica napus low oxygen stress nitrate accumulation nitrate reductase gene expression |
| title | Response of Nitrate Metabolism in Seedlings of Oilseed Rape (Brassica napus L.) to Low Oxygen Stress |
| title_full | Response of Nitrate Metabolism in Seedlings of Oilseed Rape (Brassica napus L.) to Low Oxygen Stress |
| title_fullStr | Response of Nitrate Metabolism in Seedlings of Oilseed Rape (Brassica napus L.) to Low Oxygen Stress |
| title_full_unstemmed | Response of Nitrate Metabolism in Seedlings of Oilseed Rape (Brassica napus L.) to Low Oxygen Stress |
| title_short | Response of Nitrate Metabolism in Seedlings of Oilseed Rape (Brassica napus L.) to Low Oxygen Stress |
| title_sort | response of nitrate metabolism in seedlings of oilseed rape brassica napus l to low oxygen stress |
| topic | Brassica napus low oxygen stress nitrate accumulation nitrate reductase gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S2095311913606930 |
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