Glucuronidase Gene
Gluconacetobacter diazotrophicus lives inside plant tissue cells in the form of colonies and excretes about half of the fixed nitrogen, which offers potential power that improves plant growth. The aim of this study is to find the interaction of glucuronidase (GUS)-labeled G. diazotrophicus with spin...
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| Format: | Article |
| Language: | English |
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Koya University
2024-01-01
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| Series: | ARO-The Scientific Journal of Koya University |
| Subjects: | |
| Online Access: | http://aro.koyauniversity.org/index.php/aro/article/view/1239 |
| _version_ | 1797361503023661056 |
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| author | Hikmat M. Masyab Qutaiba S. Al-Nema Mozahim Q. Al-Mallah |
| author_facet | Hikmat M. Masyab Qutaiba S. Al-Nema Mozahim Q. Al-Mallah |
| author_sort | Hikmat M. Masyab |
| collection | DOAJ |
| description | Gluconacetobacter diazotrophicus lives inside plant tissue cells in the form of colonies and excretes about half of the fixed nitrogen, which offers potential power that improves plant growth. The aim of this study is to find the interaction of glucuronidase (GUS)-labeled G. diazotrophicus with spinach seedlings and the detection of GUS genes using X-gluc dye (5-bromo-4-chloro-3-indolyl-β-D- glucuronic acid). The GUS protocol is used to detect GUS-labeled G. diazotrophicus in spinach seedling tissues by chemical detection using X-gluc dye. The results show that the spinach seedlings are successfully infected with GUS-labeled G. diazotrophicus , with the survival of the seedlings throughout their growth period and an improvement in the growth of pollinated seedlings. The outcomes of the microscopic inspection of the root slices reveal the presence of bacterial cells at the root tips and their concentration in the area of the cell walls of the peripheral cells. Furthermore, the findings of microscopic examinations of longitudinal sections for cotyledons show the presence of a number of bacteria within epidermal cell walls. This indicates that the determinants of the interaction between these bacteria and spinach seedlings are suitable for the expression of the gene responsible for the formation of the nitrogenase enzyme. |
| first_indexed | 2024-03-08T15:54:37Z |
| format | Article |
| id | doaj.art-5ccfa07e819f400aac0b1203b25ab178 |
| institution | Directory Open Access Journal |
| issn | 2410-9355 2307-549X |
| language | English |
| last_indexed | 2024-03-08T15:54:37Z |
| publishDate | 2024-01-01 |
| publisher | Koya University |
| record_format | Article |
| series | ARO-The Scientific Journal of Koya University |
| spelling | doaj.art-5ccfa07e819f400aac0b1203b25ab1782024-01-08T21:48:19ZengKoya UniversityARO-The Scientific Journal of Koya University2410-93552307-549X2024-01-0112110.14500/aro.11239Glucuronidase GeneHikmat M. Masyab0Qutaiba S. Al-Nema1Mozahim Q. Al-Mallah2Department of Biology, Faculty of Science and Health, Koya University, Koya KOY45, Kurdistan Region - F.R. IraqDepartment of Biology, College Education for Pure Sciences, University of Mosul, Mosul, F.R IraqDepartment of MLT, Al-Hadbaa University College, Mosul, F..R. IraqGluconacetobacter diazotrophicus lives inside plant tissue cells in the form of colonies and excretes about half of the fixed nitrogen, which offers potential power that improves plant growth. The aim of this study is to find the interaction of glucuronidase (GUS)-labeled G. diazotrophicus with spinach seedlings and the detection of GUS genes using X-gluc dye (5-bromo-4-chloro-3-indolyl-β-D- glucuronic acid). The GUS protocol is used to detect GUS-labeled G. diazotrophicus in spinach seedling tissues by chemical detection using X-gluc dye. The results show that the spinach seedlings are successfully infected with GUS-labeled G. diazotrophicus , with the survival of the seedlings throughout their growth period and an improvement in the growth of pollinated seedlings. The outcomes of the microscopic inspection of the root slices reveal the presence of bacterial cells at the root tips and their concentration in the area of the cell walls of the peripheral cells. Furthermore, the findings of microscopic examinations of longitudinal sections for cotyledons show the presence of a number of bacteria within epidermal cell walls. This indicates that the determinants of the interaction between these bacteria and spinach seedlings are suitable for the expression of the gene responsible for the formation of the nitrogenase enzyme.http://aro.koyauniversity.org/index.php/aro/article/view/1239Glucuronidase protocolGluconacetobacter diazotrophicusInteractionNitrogen fixationSpinach |
| spellingShingle | Hikmat M. Masyab Qutaiba S. Al-Nema Mozahim Q. Al-Mallah Glucuronidase Gene ARO-The Scientific Journal of Koya University Glucuronidase protocol Gluconacetobacter diazotrophicus Interaction Nitrogen fixation Spinach |
| title | Glucuronidase Gene |
| title_full | Glucuronidase Gene |
| title_fullStr | Glucuronidase Gene |
| title_full_unstemmed | Glucuronidase Gene |
| title_short | Glucuronidase Gene |
| title_sort | glucuronidase gene |
| topic | Glucuronidase protocol Gluconacetobacter diazotrophicus Interaction Nitrogen fixation Spinach |
| url | http://aro.koyauniversity.org/index.php/aro/article/view/1239 |
| work_keys_str_mv | AT hikmatmmasyab glucuronidasegene AT qutaibasalnema glucuronidasegene AT mozahimqalmallah glucuronidasegene |