Molecular Characteristics of Extraintestinal Pathogenic <i>E. coli</i> (ExPEC), Uropathogenic <i>E. coli</i> (UPEC), and Multidrug Resistant <i>E. coli</i> Isolated from Healthy Dogs in Spain. Whole Genome Sequencing of Canine ST372 Isolates and Comparison with Human Isolates Causing Extraintestinal Infections
Under a one health perspective and the worldwide antimicrobial resistance concern, we investigated extraintestinal pathogenic <i>Escherichia coli</i> (ExPEC), uropathogenic <i>E. coli</i> (UPEC), and multidrug resistant (MDR) <i>E. coli</i> from 197 isolates recov...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/11/1712 |
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| author | Saskia-Camille Flament-Simon María de Toro Vanesa García Jesús E. Blanco Miguel Blanco María Pilar Alonso Ana Goicoa Juan Díaz-González Marie-Hélène Nicolas-Chanoine Jorge Blanco |
| author_facet | Saskia-Camille Flament-Simon María de Toro Vanesa García Jesús E. Blanco Miguel Blanco María Pilar Alonso Ana Goicoa Juan Díaz-González Marie-Hélène Nicolas-Chanoine Jorge Blanco |
| author_sort | Saskia-Camille Flament-Simon |
| collection | DOAJ |
| description | Under a one health perspective and the worldwide antimicrobial resistance concern, we investigated extraintestinal pathogenic <i>Escherichia coli</i> (ExPEC), uropathogenic <i>E. coli</i> (UPEC), and multidrug resistant (MDR) <i>E. coli</i> from 197 isolates recovered from healthy dogs in Spain between 2013 and 2017. A total of 91 (46.2%) isolates were molecularly classified as ExPEC and/or UPEC, including 50 clones, among which (i) four clones were dominant (B2-CH14-180-ST127, B2-CH52-14-ST141, B2-CH103-9-ST372 and F-CH4-58-ST648) and (ii) 15 had been identified among isolates causing extraintestinal infections in Spanish and French humans in 2015 and 2016. A total of 28 (14.2%) isolates were classified as MDR, associated with B1, D, and E phylogroups, and included 24 clones, of which eight had also been identified among the human clinical isolates. We selected 23 ST372 strains, 21 from healthy dogs, and two from human clinical isolates for whole genome sequencing and built an SNP-tree with these 23 genomes and 174 genomes (128 from canine strains and 46 from human strains) obtained from public databases. These 197 genomes were segregated into six clusters. Cluster 1 comprised 74.6% of the strain genomes, mostly composed of canine strain genomes (<i>p</i> < 0.00001). Clusters 4 and 6 also included canine strain genomes, while clusters 2, 3, and 5 were significantly associated with human strain genomes. Finding several common clones and clone-related serotypes in dogs and humans suggests a potentially bidirectional clone transfer that argues for the one health perspective. |
| first_indexed | 2024-03-10T15:10:18Z |
| format | Article |
| id | doaj.art-5cd0043834c54214a162c2a5b65125ae |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T15:10:18Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5cd0043834c54214a162c2a5b65125ae2023-11-20T19:23:46ZengMDPI AGMicroorganisms2076-26072020-10-01811171210.3390/microorganisms8111712Molecular Characteristics of Extraintestinal Pathogenic <i>E. coli</i> (ExPEC), Uropathogenic <i>E. coli</i> (UPEC), and Multidrug Resistant <i>E. coli</i> Isolated from Healthy Dogs in Spain. Whole Genome Sequencing of Canine ST372 Isolates and Comparison with Human Isolates Causing Extraintestinal InfectionsSaskia-Camille Flament-Simon0María de Toro1Vanesa García2Jesús E. Blanco3Miguel Blanco4María Pilar Alonso5Ana Goicoa6Juan Díaz-González7Marie-Hélène Nicolas-Chanoine8Jorge Blanco9Laboratorio de Referencia de E. coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, SpainPlataforma de Genómica y Bioinformática, Centro de Investigación Biomédica de La Rioja (CIBIR), 26006 Logroño, SpainLaboratorio de Referencia de E. coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, SpainLaboratorio de Referencia de E. coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, SpainLaboratorio de Referencia de E. coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, SpainInstituto de Investigación Sanitaria de Santiago de Compostela (IDIS), 15706 Santiago de Compostela, SpainServicio de Medicina Interna, Hospital Veterinario Universitario Rof Codina, USC, 27002 Lugo, SpainLaboratorio de Referencia de E. coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, SpainUniversité de Paris, IAME, UMR1137, INSERM, F-75018 Paris, FranceLaboratorio de Referencia de E. coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, SpainUnder a one health perspective and the worldwide antimicrobial resistance concern, we investigated extraintestinal pathogenic <i>Escherichia coli</i> (ExPEC), uropathogenic <i>E. coli</i> (UPEC), and multidrug resistant (MDR) <i>E. coli</i> from 197 isolates recovered from healthy dogs in Spain between 2013 and 2017. A total of 91 (46.2%) isolates were molecularly classified as ExPEC and/or UPEC, including 50 clones, among which (i) four clones were dominant (B2-CH14-180-ST127, B2-CH52-14-ST141, B2-CH103-9-ST372 and F-CH4-58-ST648) and (ii) 15 had been identified among isolates causing extraintestinal infections in Spanish and French humans in 2015 and 2016. A total of 28 (14.2%) isolates were classified as MDR, associated with B1, D, and E phylogroups, and included 24 clones, of which eight had also been identified among the human clinical isolates. We selected 23 ST372 strains, 21 from healthy dogs, and two from human clinical isolates for whole genome sequencing and built an SNP-tree with these 23 genomes and 174 genomes (128 from canine strains and 46 from human strains) obtained from public databases. These 197 genomes were segregated into six clusters. Cluster 1 comprised 74.6% of the strain genomes, mostly composed of canine strain genomes (<i>p</i> < 0.00001). Clusters 4 and 6 also included canine strain genomes, while clusters 2, 3, and 5 were significantly associated with human strain genomes. Finding several common clones and clone-related serotypes in dogs and humans suggests a potentially bidirectional clone transfer that argues for the one health perspective.https://www.mdpi.com/2076-2607/8/11/1712<i>Escherichia coli</i>dogsvirulence genesantibiotic resistanceWGSST372 |
| spellingShingle | Saskia-Camille Flament-Simon María de Toro Vanesa García Jesús E. Blanco Miguel Blanco María Pilar Alonso Ana Goicoa Juan Díaz-González Marie-Hélène Nicolas-Chanoine Jorge Blanco Molecular Characteristics of Extraintestinal Pathogenic <i>E. coli</i> (ExPEC), Uropathogenic <i>E. coli</i> (UPEC), and Multidrug Resistant <i>E. coli</i> Isolated from Healthy Dogs in Spain. Whole Genome Sequencing of Canine ST372 Isolates and Comparison with Human Isolates Causing Extraintestinal Infections Microorganisms <i>Escherichia coli</i> dogs virulence genes antibiotic resistance WGS ST372 |
| title | Molecular Characteristics of Extraintestinal Pathogenic <i>E. coli</i> (ExPEC), Uropathogenic <i>E. coli</i> (UPEC), and Multidrug Resistant <i>E. coli</i> Isolated from Healthy Dogs in Spain. Whole Genome Sequencing of Canine ST372 Isolates and Comparison with Human Isolates Causing Extraintestinal Infections |
| title_full | Molecular Characteristics of Extraintestinal Pathogenic <i>E. coli</i> (ExPEC), Uropathogenic <i>E. coli</i> (UPEC), and Multidrug Resistant <i>E. coli</i> Isolated from Healthy Dogs in Spain. Whole Genome Sequencing of Canine ST372 Isolates and Comparison with Human Isolates Causing Extraintestinal Infections |
| title_fullStr | Molecular Characteristics of Extraintestinal Pathogenic <i>E. coli</i> (ExPEC), Uropathogenic <i>E. coli</i> (UPEC), and Multidrug Resistant <i>E. coli</i> Isolated from Healthy Dogs in Spain. Whole Genome Sequencing of Canine ST372 Isolates and Comparison with Human Isolates Causing Extraintestinal Infections |
| title_full_unstemmed | Molecular Characteristics of Extraintestinal Pathogenic <i>E. coli</i> (ExPEC), Uropathogenic <i>E. coli</i> (UPEC), and Multidrug Resistant <i>E. coli</i> Isolated from Healthy Dogs in Spain. Whole Genome Sequencing of Canine ST372 Isolates and Comparison with Human Isolates Causing Extraintestinal Infections |
| title_short | Molecular Characteristics of Extraintestinal Pathogenic <i>E. coli</i> (ExPEC), Uropathogenic <i>E. coli</i> (UPEC), and Multidrug Resistant <i>E. coli</i> Isolated from Healthy Dogs in Spain. Whole Genome Sequencing of Canine ST372 Isolates and Comparison with Human Isolates Causing Extraintestinal Infections |
| title_sort | molecular characteristics of extraintestinal pathogenic i e coli i expec uropathogenic i e coli i upec and multidrug resistant i e coli i isolated from healthy dogs in spain whole genome sequencing of canine st372 isolates and comparison with human isolates causing extraintestinal infections |
| topic | <i>Escherichia coli</i> dogs virulence genes antibiotic resistance WGS ST372 |
| url | https://www.mdpi.com/2076-2607/8/11/1712 |
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