Synergistic Combination of Ciplukan (Physalis angulata) Herbs Ethanolic Extract and Doxorubicin on T47D Breast Cancer Cells
Doxorubicin is one of chemotherapeutic agent widely used in breast cancer treatment, but in high dose doxorubicin gives negative side effect, including vomit, nausea, immune suppression, and cardiac toxicity. This toxicity hopefully could be reduced by combination chemotherapy using natural herbs su...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Cancer Chemoprevention
2010-02-01
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| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/5 |
| _version_ | 1817976392457912320 |
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| author | Inna Armandari Kartika Dyah Palupi Sofa Farida Adam Hermawan Ratna Asmah Susidarti Edy Meiyanto |
| author_facet | Inna Armandari Kartika Dyah Palupi Sofa Farida Adam Hermawan Ratna Asmah Susidarti Edy Meiyanto |
| author_sort | Inna Armandari |
| collection | DOAJ |
| description | Doxorubicin is one of chemotherapeutic agent widely used in breast cancer treatment, but in high dose doxorubicin gives negative side effect, including vomit, nausea, immune suppression, and cardiac toxicity. This toxicity hopefully could be reduced by combination chemotherapy using natural herbs such as ciplukan herb. This research was conducted to explore cytotoxic activity of single ciplukan herbs ethanolic extract and its combination with doxorubicin on T47D breast cancer cells. Cytotoxic activity of ciplukan herbs ethanolic extract only and its combination with doxorubicin were tested on T47D cells using MTT assay to obtain IC50 value and combination index (CI), respectively. Single extract showed cytotoxic activity on T47D cells with IC50 value of was 160 µg/ml. Thus, combination treatment from ciplukan herbs ethanolic extract and doxorubicin showed synergistic effect (CI<1,0). This effect was reached at concentration of ciplukan herbs ethanolic extract-doxorubicin 80 μg/ml- 2 nM, 80 μg/ml-4 nM, and 80 μg/ml-8 nM. This research indicated that ciplukan herbs ethanolic extract is potential to be applied as co-chemotherapeutic agent in breast cancer therapy.
Key word : ciplukan herbs, doxorubicin, co-chemotherapy, T47D cells |
| first_indexed | 2024-04-13T22:02:00Z |
| format | Article |
| id | doaj.art-5cd0d50ef74c463eb1782d0a13a51ccd |
| institution | Directory Open Access Journal |
| issn | 2088-0197 2355-8989 |
| language | English |
| last_indexed | 2024-04-13T22:02:00Z |
| publishDate | 2010-02-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj.art-5cd0d50ef74c463eb1782d0a13a51ccd2022-12-22T02:28:03ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892010-02-0111263110.14499/indonesianjcanchemoprev1iss1pp26-316Synergistic Combination of Ciplukan (Physalis angulata) Herbs Ethanolic Extract and Doxorubicin on T47D Breast Cancer CellsInna Armandari0Kartika Dyah Palupi1Sofa Farida2Adam Hermawan3Ratna Asmah Susidarti4Edy Meiyanto5Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaDoxorubicin is one of chemotherapeutic agent widely used in breast cancer treatment, but in high dose doxorubicin gives negative side effect, including vomit, nausea, immune suppression, and cardiac toxicity. This toxicity hopefully could be reduced by combination chemotherapy using natural herbs such as ciplukan herb. This research was conducted to explore cytotoxic activity of single ciplukan herbs ethanolic extract and its combination with doxorubicin on T47D breast cancer cells. Cytotoxic activity of ciplukan herbs ethanolic extract only and its combination with doxorubicin were tested on T47D cells using MTT assay to obtain IC50 value and combination index (CI), respectively. Single extract showed cytotoxic activity on T47D cells with IC50 value of was 160 µg/ml. Thus, combination treatment from ciplukan herbs ethanolic extract and doxorubicin showed synergistic effect (CI<1,0). This effect was reached at concentration of ciplukan herbs ethanolic extract-doxorubicin 80 μg/ml- 2 nM, 80 μg/ml-4 nM, and 80 μg/ml-8 nM. This research indicated that ciplukan herbs ethanolic extract is potential to be applied as co-chemotherapeutic agent in breast cancer therapy. Key word : ciplukan herbs, doxorubicin, co-chemotherapy, T47D cellshttps://ijcc.chemoprev.org/index.php/ijcc/article/view/5 |
| spellingShingle | Inna Armandari Kartika Dyah Palupi Sofa Farida Adam Hermawan Ratna Asmah Susidarti Edy Meiyanto Synergistic Combination of Ciplukan (Physalis angulata) Herbs Ethanolic Extract and Doxorubicin on T47D Breast Cancer Cells ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | Synergistic Combination of Ciplukan (Physalis angulata) Herbs Ethanolic Extract and Doxorubicin on T47D Breast Cancer Cells |
| title_full | Synergistic Combination of Ciplukan (Physalis angulata) Herbs Ethanolic Extract and Doxorubicin on T47D Breast Cancer Cells |
| title_fullStr | Synergistic Combination of Ciplukan (Physalis angulata) Herbs Ethanolic Extract and Doxorubicin on T47D Breast Cancer Cells |
| title_full_unstemmed | Synergistic Combination of Ciplukan (Physalis angulata) Herbs Ethanolic Extract and Doxorubicin on T47D Breast Cancer Cells |
| title_short | Synergistic Combination of Ciplukan (Physalis angulata) Herbs Ethanolic Extract and Doxorubicin on T47D Breast Cancer Cells |
| title_sort | synergistic combination of ciplukan physalis angulata herbs ethanolic extract and doxorubicin on t47d breast cancer cells |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/5 |
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