Interactions Between Spore Morphogenetic Mutations Affect Cell Types, Sporulation, and Pathogenesis in Magnaporthe grisea
We have previously defined four single-gene mutations, con1, con2, con4, and con7, that control various stages of spore morphogenesis in the rice blast fungus. To delineate the developmental pathway of spore morphogenesis, we investigated the interactions among these morphogenetic genes by generatin...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
1998-03-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.3.199 |
| _version_ | 1818757335890264064 |
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| author | Zhixin Shi David Christian Hei Leung |
| author_facet | Zhixin Shi David Christian Hei Leung |
| author_sort | Zhixin Shi |
| collection | DOAJ |
| description | We have previously defined four single-gene mutations, con1, con2, con4, and con7, that control various stages of spore morphogenesis in the rice blast fungus. To delineate the developmental pathway of spore morphogenesis, we investigated the interactions among these morphogenetic genes by generating strains with double mutations via transformation-mediated gene disruption. Plasmids containing portions of the inactivated CON4 and CON7 genes were introduced into strains harboring single mutation to produce double mutants. Interaction between con1 and con4 resulted in reduced vegetative growth and suppression of sporulation. Interaction between con1 and con7 suppressed spore production but not vegetative growth. The con2/con4 and con4/con7 double mutants produced hybrid spore types with characteristic features of both parental mutants. The con2/con7 mutant produced con2 type spores, indicating that con2 is epistatic to con7 in spore morphogenesis. The epistatic relationship, however, reversed when traits related to pathogenesis were considered. Double mutants harboring the con7 mutation could not form appressoria or colonize plant tissue, indicating that con7 is epistatic to con2 and con4 in appressorium formation and pathogenesis. Thus, morphogenetic genes interact at multiple levels leading to different epistatic relationships in the pathways of spore morphogenesis, appressorium formation, and pathogenesis. |
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| id | doaj.art-5cd223b714b94154a2c93a7dd5806258 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-18T06:09:18Z |
| publishDate | 1998-03-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-5cd223b714b94154a2c93a7dd58062582022-12-21T21:18:27ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061998-03-0111319920710.1094/MPMI.1998.11.3.199Interactions Between Spore Morphogenetic Mutations Affect Cell Types, Sporulation, and Pathogenesis in Magnaporthe griseaZhixin ShiDavid ChristianHei LeungWe have previously defined four single-gene mutations, con1, con2, con4, and con7, that control various stages of spore morphogenesis in the rice blast fungus. To delineate the developmental pathway of spore morphogenesis, we investigated the interactions among these morphogenetic genes by generating strains with double mutations via transformation-mediated gene disruption. Plasmids containing portions of the inactivated CON4 and CON7 genes were introduced into strains harboring single mutation to produce double mutants. Interaction between con1 and con4 resulted in reduced vegetative growth and suppression of sporulation. Interaction between con1 and con7 suppressed spore production but not vegetative growth. The con2/con4 and con4/con7 double mutants produced hybrid spore types with characteristic features of both parental mutants. The con2/con7 mutant produced con2 type spores, indicating that con2 is epistatic to con7 in spore morphogenesis. The epistatic relationship, however, reversed when traits related to pathogenesis were considered. Double mutants harboring the con7 mutation could not form appressoria or colonize plant tissue, indicating that con7 is epistatic to con2 and con4 in appressorium formation and pathogenesis. Thus, morphogenetic genes interact at multiple levels leading to different epistatic relationships in the pathways of spore morphogenesis, appressorium formation, and pathogenesis.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.3.199conidiafungal developmentpathogenicityPyricularia grisea |
| spellingShingle | Zhixin Shi David Christian Hei Leung Interactions Between Spore Morphogenetic Mutations Affect Cell Types, Sporulation, and Pathogenesis in Magnaporthe grisea Molecular Plant-Microbe Interactions conidia fungal development pathogenicity Pyricularia grisea |
| title | Interactions Between Spore Morphogenetic Mutations Affect Cell Types, Sporulation, and Pathogenesis in Magnaporthe grisea |
| title_full | Interactions Between Spore Morphogenetic Mutations Affect Cell Types, Sporulation, and Pathogenesis in Magnaporthe grisea |
| title_fullStr | Interactions Between Spore Morphogenetic Mutations Affect Cell Types, Sporulation, and Pathogenesis in Magnaporthe grisea |
| title_full_unstemmed | Interactions Between Spore Morphogenetic Mutations Affect Cell Types, Sporulation, and Pathogenesis in Magnaporthe grisea |
| title_short | Interactions Between Spore Morphogenetic Mutations Affect Cell Types, Sporulation, and Pathogenesis in Magnaporthe grisea |
| title_sort | interactions between spore morphogenetic mutations affect cell types sporulation and pathogenesis in magnaporthe grisea |
| topic | conidia fungal development pathogenicity Pyricularia grisea |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.3.199 |
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