Enhancing the Cell-Free Expression of Native Membrane Proteins by In Silico Optimization of the Coding Sequence—An Experimental Study of the Human Voltage-Dependent Anion Channel

Enhancing the Cell-Free Expression of Native Membrane Proteins by In Silico Optimization of the Coding Sequence—An Experimental Study of the Human Voltage-Dependent Anion Channel

Membrane proteins are involved in many aspects of cellular biology; for example, they regulate how cells interact with their environment, so such proteins are important drug targets. The rapid advancement in the field of immune effector cell therapy has been expanding the horizons of synthetic membr...

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Bibliographic Details
Main Authors: Sonja Zayni, Samar Damiati, Susana Moreno-Flores, Fabian Amman, Ivo Hofacker, David Jin, Eva-Kathrin Ehmoser
Format: Article
Language:English
Published: MDPI AG 2021-09-01
Series:Membranes
Subjects:
cell-free membrane protein expression
translation enhancer
translation initiation
ribosome docking site
sequence design
Online Access:https://www.mdpi.com/2077-0375/11/10/741

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