Global analysis of the association between pig muscle fatty acid composition and gene expression using RNA-Seq
Abstract Fatty acids (FAs) play an essential role as mediators of cell signaling and signal transduction, affecting metabolic homeostasis and determining meat quality in pigs. However, FAs are transformed by the action of several genes, such as those encoding desaturases and elongases of FAs in lipo...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-27016-x |
| _version_ | 1797952480807485440 |
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| author | Jesús Valdés-Hernández Yuliaxis Ramayo-Caldas Magí Passols Cristina Sebastià Lourdes Criado-Mesas Daniel Crespo-Piazuelo Anna Esteve-Codina Anna Castelló Armand Sánchez Josep M. Folch |
| author_facet | Jesús Valdés-Hernández Yuliaxis Ramayo-Caldas Magí Passols Cristina Sebastià Lourdes Criado-Mesas Daniel Crespo-Piazuelo Anna Esteve-Codina Anna Castelló Armand Sánchez Josep M. Folch |
| author_sort | Jesús Valdés-Hernández |
| collection | DOAJ |
| description | Abstract Fatty acids (FAs) play an essential role as mediators of cell signaling and signal transduction, affecting metabolic homeostasis and determining meat quality in pigs. However, FAs are transformed by the action of several genes, such as those encoding desaturases and elongases of FAs in lipogenic tissues. The aim of the current work was to identify candidate genes, biological processes, and pathways involved in the modulation of intramuscular FA profile from longissimus dorsi muscle. FA profile by gas chromatography of methyl esters and gene expression by RNA-Seq were determined in 129 Iberian × Duroc backcrossed pigs. An association analysis between the muscle transcriptome and its FA profile was performed, followed by a concordance and functional analysis. Overall, a list of well-known (e.g., PLIN1, LEP, ELOVL6, SC5D, NCOA2, ACSL1, MDH1, LPL, LGALS12, TFRC, GOT1, and FBP1) and novel (e.g., TRARG1, TANK, ENSSSCG00000011196, and ENSSSCG00000038429) candidate genes was identified, either in association with specific or several FA traits. Likewise, several of these genes belong to biological processes and pathways linked to energy, lipid, and carbohydrate metabolism, which seem determinants in the modulation of FA compositions. This study can contribute to elucidate the complex relationship between gene expression and FA profile in pig muscle. |
| first_indexed | 2024-04-10T22:48:07Z |
| format | Article |
| id | doaj.art-5cdbd7f03bfb45859706a617ba088f40 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-10T22:48:07Z |
| publishDate | 2023-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5cdbd7f03bfb45859706a617ba088f402023-01-15T12:12:39ZengNature PortfolioScientific Reports2045-23222023-01-0113111210.1038/s41598-022-27016-xGlobal analysis of the association between pig muscle fatty acid composition and gene expression using RNA-SeqJesús Valdés-Hernández0Yuliaxis Ramayo-Caldas1Magí Passols2Cristina Sebastià3Lourdes Criado-Mesas4Daniel Crespo-Piazuelo5Anna Esteve-Codina6Anna Castelló7Armand Sánchez8Josep M. Folch9Plant and Animal Genomics, Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UBDepartament de Genètica i Millora Animal, Institut de Recerca y Tecnologia Agraroalimentàries (IRTA)Plant and Animal Genomics, Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UBPlant and Animal Genomics, Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UBPlant and Animal Genomics, Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UBDepartament de Genètica i Millora Animal, Institut de Recerca y Tecnologia Agraroalimentàries (IRTA)CNAG-CRG, Centre for Genomic Regulation, Barcelona Institute of Science and TechnologyPlant and Animal Genomics, Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UBPlant and Animal Genomics, Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UBPlant and Animal Genomics, Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UBAbstract Fatty acids (FAs) play an essential role as mediators of cell signaling and signal transduction, affecting metabolic homeostasis and determining meat quality in pigs. However, FAs are transformed by the action of several genes, such as those encoding desaturases and elongases of FAs in lipogenic tissues. The aim of the current work was to identify candidate genes, biological processes, and pathways involved in the modulation of intramuscular FA profile from longissimus dorsi muscle. FA profile by gas chromatography of methyl esters and gene expression by RNA-Seq were determined in 129 Iberian × Duroc backcrossed pigs. An association analysis between the muscle transcriptome and its FA profile was performed, followed by a concordance and functional analysis. Overall, a list of well-known (e.g., PLIN1, LEP, ELOVL6, SC5D, NCOA2, ACSL1, MDH1, LPL, LGALS12, TFRC, GOT1, and FBP1) and novel (e.g., TRARG1, TANK, ENSSSCG00000011196, and ENSSSCG00000038429) candidate genes was identified, either in association with specific or several FA traits. Likewise, several of these genes belong to biological processes and pathways linked to energy, lipid, and carbohydrate metabolism, which seem determinants in the modulation of FA compositions. This study can contribute to elucidate the complex relationship between gene expression and FA profile in pig muscle.https://doi.org/10.1038/s41598-022-27016-x |
| spellingShingle | Jesús Valdés-Hernández Yuliaxis Ramayo-Caldas Magí Passols Cristina Sebastià Lourdes Criado-Mesas Daniel Crespo-Piazuelo Anna Esteve-Codina Anna Castelló Armand Sánchez Josep M. Folch Global analysis of the association between pig muscle fatty acid composition and gene expression using RNA-Seq Scientific Reports |
| title | Global analysis of the association between pig muscle fatty acid composition and gene expression using RNA-Seq |
| title_full | Global analysis of the association between pig muscle fatty acid composition and gene expression using RNA-Seq |
| title_fullStr | Global analysis of the association between pig muscle fatty acid composition and gene expression using RNA-Seq |
| title_full_unstemmed | Global analysis of the association between pig muscle fatty acid composition and gene expression using RNA-Seq |
| title_short | Global analysis of the association between pig muscle fatty acid composition and gene expression using RNA-Seq |
| title_sort | global analysis of the association between pig muscle fatty acid composition and gene expression using rna seq |
| url | https://doi.org/10.1038/s41598-022-27016-x |
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