Molecular Characterization and Prevalence of Antimicrobial-Resistant <i>Escherichia coli</i> Isolates Derived from Clinical Specimens and Environmental Habitats
Antibiotic-resistant bacteria (ARB) are present in wastewaters as their elimination during treatment in wastewater treatment plants (WWTPs) is often impossible. Water plays an important role in the spread of these microorganisms among humans, animals and the environment. This study aimed to assess t...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/6/1399 |
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| author | Chrysoula Dioli Olga Pappa Eirini Siatravani Spyridoula Bratakou Apostolos Tatsiopoulos Panagiota Giakkoupi Vivi Miriagou Apostolos Beloukas |
| author_facet | Chrysoula Dioli Olga Pappa Eirini Siatravani Spyridoula Bratakou Apostolos Tatsiopoulos Panagiota Giakkoupi Vivi Miriagou Apostolos Beloukas |
| author_sort | Chrysoula Dioli |
| collection | DOAJ |
| description | Antibiotic-resistant bacteria (ARB) are present in wastewaters as their elimination during treatment in wastewater treatment plants (WWTPs) is often impossible. Water plays an important role in the spread of these microorganisms among humans, animals and the environment. This study aimed to assess the antimicrobial resistance patterns, resistance genes and molecular genotypes by means of phylogenetic groups of <i>E. coli</i> isolates in aquatic habitats, including sewage and receiving water bodies, as well as clinical settings in the Boeotia regional district of Greece. The highest resistance rates among both environmental and clinical isolates were observed to be for penicillins, ampicillin and piperacillin. Resistance patterns related to extended spectrum β-lactamases (ESBL) production and ESBL genes were also detected in both environmental and clinical isolates. Phylogenetic group B2 was predominant in clinical settings and the second most frequent among wastewaters, whereas group A was dominant in all environmental isolates. In conclusion, the studied river water and wastewaters may serve as reservoirs of resistant <i>E. coli</i> isolates that pose potential threats to both human and animal health. |
| first_indexed | 2024-03-11T02:08:13Z |
| format | Article |
| id | doaj.art-5cdc36f59a0a4ba5b1440e11fd95bbda |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T02:08:13Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5cdc36f59a0a4ba5b1440e11fd95bbda2023-11-18T11:41:21ZengMDPI AGMicroorganisms2076-26072023-05-01116139910.3390/microorganisms11061399Molecular Characterization and Prevalence of Antimicrobial-Resistant <i>Escherichia coli</i> Isolates Derived from Clinical Specimens and Environmental HabitatsChrysoula Dioli0Olga Pappa1Eirini Siatravani2Spyridoula Bratakou3Apostolos Tatsiopoulos4Panagiota Giakkoupi5Vivi Miriagou6Apostolos Beloukas7Molecular Microbiology and Immunology Laboratory, Department of Biomedical Sciences, University of West Attica, 28 Ag. Spyridonos Str., 12243 Athens, GreeceMolecular Microbiology and Immunology Laboratory, Department of Biomedical Sciences, University of West Attica, 28 Ag. Spyridonos Str., 12243 Athens, GreeceMolecular Microbiology and Immunology Laboratory, Department of Biomedical Sciences, University of West Attica, 28 Ag. Spyridonos Str., 12243 Athens, GreeceMolecular Microbiology and Immunology Laboratory, Department of Biomedical Sciences, University of West Attica, 28 Ag. Spyridonos Str., 12243 Athens, GreeceMicrobiology Laboratory, General Hospital of Livadeia, Agiou Vlasiou, 32100 Livadia, GreeceDepartment of Public Health Policy, School of Public Health, University of West Attica, 196 Alexandras Avenue, 11521 Athens, GreeceLaboratory of Bacteriology, Hellenic Pasteur Institute, 127 Vasilissis Sofias Avenue, 11521 Athens, GreeceMolecular Microbiology and Immunology Laboratory, Department of Biomedical Sciences, University of West Attica, 28 Ag. Spyridonos Str., 12243 Athens, GreeceAntibiotic-resistant bacteria (ARB) are present in wastewaters as their elimination during treatment in wastewater treatment plants (WWTPs) is often impossible. Water plays an important role in the spread of these microorganisms among humans, animals and the environment. This study aimed to assess the antimicrobial resistance patterns, resistance genes and molecular genotypes by means of phylogenetic groups of <i>E. coli</i> isolates in aquatic habitats, including sewage and receiving water bodies, as well as clinical settings in the Boeotia regional district of Greece. The highest resistance rates among both environmental and clinical isolates were observed to be for penicillins, ampicillin and piperacillin. Resistance patterns related to extended spectrum β-lactamases (ESBL) production and ESBL genes were also detected in both environmental and clinical isolates. Phylogenetic group B2 was predominant in clinical settings and the second most frequent among wastewaters, whereas group A was dominant in all environmental isolates. In conclusion, the studied river water and wastewaters may serve as reservoirs of resistant <i>E. coli</i> isolates that pose potential threats to both human and animal health.https://www.mdpi.com/2076-2607/11/6/1399<i>E. coli</i>environmentantibiotic resistanceantibiotic resistance genes |
| spellingShingle | Chrysoula Dioli Olga Pappa Eirini Siatravani Spyridoula Bratakou Apostolos Tatsiopoulos Panagiota Giakkoupi Vivi Miriagou Apostolos Beloukas Molecular Characterization and Prevalence of Antimicrobial-Resistant <i>Escherichia coli</i> Isolates Derived from Clinical Specimens and Environmental Habitats Microorganisms <i>E. coli</i> environment antibiotic resistance antibiotic resistance genes |
| title | Molecular Characterization and Prevalence of Antimicrobial-Resistant <i>Escherichia coli</i> Isolates Derived from Clinical Specimens and Environmental Habitats |
| title_full | Molecular Characterization and Prevalence of Antimicrobial-Resistant <i>Escherichia coli</i> Isolates Derived from Clinical Specimens and Environmental Habitats |
| title_fullStr | Molecular Characterization and Prevalence of Antimicrobial-Resistant <i>Escherichia coli</i> Isolates Derived from Clinical Specimens and Environmental Habitats |
| title_full_unstemmed | Molecular Characterization and Prevalence of Antimicrobial-Resistant <i>Escherichia coli</i> Isolates Derived from Clinical Specimens and Environmental Habitats |
| title_short | Molecular Characterization and Prevalence of Antimicrobial-Resistant <i>Escherichia coli</i> Isolates Derived from Clinical Specimens and Environmental Habitats |
| title_sort | molecular characterization and prevalence of antimicrobial resistant i escherichia coli i isolates derived from clinical specimens and environmental habitats |
| topic | <i>E. coli</i> environment antibiotic resistance antibiotic resistance genes |
| url | https://www.mdpi.com/2076-2607/11/6/1399 |
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