Roles of the adenosine receptor and CD73 in the regulatory effect of γδ T cells.
The adenosine A2A receptor (A2AR), the main functional adenosine receptor on murine T cells, plays a unique role in the attenuation of inflammation and tissue damage in vivo. Here, we showed that, of the immune cell types tested, activated γδ T cells expressed the highest levels of A2AR mRNA and tha...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4182534?pdf=render |
| _version_ | 1818434847328174080 |
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| author | Dongchun Liang Aijun Zuo Hui Shao Mingjiazi Chen Henry J Kaplan Deming Sun |
| author_facet | Dongchun Liang Aijun Zuo Hui Shao Mingjiazi Chen Henry J Kaplan Deming Sun |
| author_sort | Dongchun Liang |
| collection | DOAJ |
| description | The adenosine A2A receptor (A2AR), the main functional adenosine receptor on murine T cells, plays a unique role in the attenuation of inflammation and tissue damage in vivo. Here, we showed that, of the immune cell types tested, activated γδ T cells expressed the highest levels of A2AR mRNA and that A2AR ligation inhibited αβ T cell activation, but enhanced γδ T cell activation. We also showed that the inhibitory effect of an adenosine receptor agonist on autoreactive T cells was prevented by addition of a low percentage of activated γδ T cells. Furthermore, compared to resting cells, activated γδ T cells expressed significantly lower levels of CD73, an enzyme involved in the generation of extracellular adenosine. Exogenous AMP had a significant inhibitory effect on autoreactive T cell responses, but only in the presence of CD73+ γδ T cells, and this effect was abolished by a CD73 inhibitor. Our results show that expression of increased amounts of A2AR allows γδ T cells to bind adenosine and thereby attenuate its suppressive effect, while decreased expression of CD73 results in less generation of adenosine in the inflammatory site. Together, these events allow activated γδ T cells to acquire increased proinflammatory activity, leading to augmented autoimmune responses. |
| first_indexed | 2024-12-14T16:43:29Z |
| format | Article |
| id | doaj.art-5ce10823aad54cd9a6269d3dcdf2c29d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T16:43:29Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5ce10823aad54cd9a6269d3dcdf2c29d2022-12-21T22:54:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0199e10893210.1371/journal.pone.0108932Roles of the adenosine receptor and CD73 in the regulatory effect of γδ T cells.Dongchun LiangAijun ZuoHui ShaoMingjiazi ChenHenry J KaplanDeming SunThe adenosine A2A receptor (A2AR), the main functional adenosine receptor on murine T cells, plays a unique role in the attenuation of inflammation and tissue damage in vivo. Here, we showed that, of the immune cell types tested, activated γδ T cells expressed the highest levels of A2AR mRNA and that A2AR ligation inhibited αβ T cell activation, but enhanced γδ T cell activation. We also showed that the inhibitory effect of an adenosine receptor agonist on autoreactive T cells was prevented by addition of a low percentage of activated γδ T cells. Furthermore, compared to resting cells, activated γδ T cells expressed significantly lower levels of CD73, an enzyme involved in the generation of extracellular adenosine. Exogenous AMP had a significant inhibitory effect on autoreactive T cell responses, but only in the presence of CD73+ γδ T cells, and this effect was abolished by a CD73 inhibitor. Our results show that expression of increased amounts of A2AR allows γδ T cells to bind adenosine and thereby attenuate its suppressive effect, while decreased expression of CD73 results in less generation of adenosine in the inflammatory site. Together, these events allow activated γδ T cells to acquire increased proinflammatory activity, leading to augmented autoimmune responses.http://europepmc.org/articles/PMC4182534?pdf=render |
| spellingShingle | Dongchun Liang Aijun Zuo Hui Shao Mingjiazi Chen Henry J Kaplan Deming Sun Roles of the adenosine receptor and CD73 in the regulatory effect of γδ T cells. PLoS ONE |
| title | Roles of the adenosine receptor and CD73 in the regulatory effect of γδ T cells. |
| title_full | Roles of the adenosine receptor and CD73 in the regulatory effect of γδ T cells. |
| title_fullStr | Roles of the adenosine receptor and CD73 in the regulatory effect of γδ T cells. |
| title_full_unstemmed | Roles of the adenosine receptor and CD73 in the regulatory effect of γδ T cells. |
| title_short | Roles of the adenosine receptor and CD73 in the regulatory effect of γδ T cells. |
| title_sort | roles of the adenosine receptor and cd73 in the regulatory effect of γδ t cells |
| url | http://europepmc.org/articles/PMC4182534?pdf=render |
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