Effects of dehydroabietic acid on nontarget lipidomics and proteomics of HepG2
Objective: Studies of the effects of dehydroabietic acid on the multiomics of HepG2 hepatoma carcinoma cells are currently lacking. In this study, the molecular mechanism of the influence of dehydroabietic acid on HepG2 cells was disclosed by studying lipidomics and proteomics. Correlations among mu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.1015240/full |
| _version_ | 1828098068822622208 |
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| author | Zhikai Xiahou Jun Han Jun Han |
| author_facet | Zhikai Xiahou Jun Han Jun Han |
| author_sort | Zhikai Xiahou |
| collection | DOAJ |
| description | Objective: Studies of the effects of dehydroabietic acid on the multiomics of HepG2 hepatoma carcinoma cells are currently lacking. In this study, the molecular mechanism of the influence of dehydroabietic acid on HepG2 cells was disclosed by studying lipidomics and proteomics. Correlations among multiomics conjoint analysis results were verified.Methods: First, proteomics analysis of HepG2 cells was carried out using dehydroabietic acid. Differentially expressed proteins were screened and analyzed. Pathway enrichment analyses of differential proteins were compared, and the molecular mechanism was disclosed. Second, lipidomics analysis of HepG2 cells was conducted using dehydroabietic acid. The influence of dehydroabietic acid on HepG2 cells was determined on the lipid molecular level. Finally, a conjoint analysis of data related to differentially expressed proteins of ferroptosis and differentially changing lipid molecules was implemented.Results: A total of 260 upregulated and 961 downregulated proteins were screened in the proteomics analysis. The top five significantly enriched pathways included ferroptosis, oxidative phosphorylation, and protein processing in the endoplasmic reticulum. In the lipidomics analysis, 30 significantly differential metabolites with upregulated and downregulated expression were identified, and differentially expressed lipids were mainly related to the metabolism of glyceryl phosphatide. According to the comprehensive multiomics analysis results, real-time quantitative PCR and the enzyme-linked immunosorbent assay (ELISA), ACSL3 participated in cardiolipin metabolism.Conclusion: Dehydroabietic acid influences HepG2 cells through the above biological pathways. |
| first_indexed | 2024-04-11T07:57:02Z |
| format | Article |
| id | doaj.art-5ce417a4f5f54f3fa4a51b17ddaf6ba0 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-11T07:57:02Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-5ce417a4f5f54f3fa4a51b17ddaf6ba02022-12-22T04:35:54ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-12-011310.3389/fphar.2022.10152401015240Effects of dehydroabietic acid on nontarget lipidomics and proteomics of HepG2Zhikai Xiahou0Jun Han1Jun Han2China Institute of Sport and Health Science, Beijing Sport University, Beijing, ChinaBeijing Tcmages Pharmaceutical Co.Ltd., Beijing, ChinaBeijing Kangrentang Pharmaceutical Co.,Ltd., Beijing, ChinaObjective: Studies of the effects of dehydroabietic acid on the multiomics of HepG2 hepatoma carcinoma cells are currently lacking. In this study, the molecular mechanism of the influence of dehydroabietic acid on HepG2 cells was disclosed by studying lipidomics and proteomics. Correlations among multiomics conjoint analysis results were verified.Methods: First, proteomics analysis of HepG2 cells was carried out using dehydroabietic acid. Differentially expressed proteins were screened and analyzed. Pathway enrichment analyses of differential proteins were compared, and the molecular mechanism was disclosed. Second, lipidomics analysis of HepG2 cells was conducted using dehydroabietic acid. The influence of dehydroabietic acid on HepG2 cells was determined on the lipid molecular level. Finally, a conjoint analysis of data related to differentially expressed proteins of ferroptosis and differentially changing lipid molecules was implemented.Results: A total of 260 upregulated and 961 downregulated proteins were screened in the proteomics analysis. The top five significantly enriched pathways included ferroptosis, oxidative phosphorylation, and protein processing in the endoplasmic reticulum. In the lipidomics analysis, 30 significantly differential metabolites with upregulated and downregulated expression were identified, and differentially expressed lipids were mainly related to the metabolism of glyceryl phosphatide. According to the comprehensive multiomics analysis results, real-time quantitative PCR and the enzyme-linked immunosorbent assay (ELISA), ACSL3 participated in cardiolipin metabolism.Conclusion: Dehydroabietic acid influences HepG2 cells through the above biological pathways.https://www.frontiersin.org/articles/10.3389/fphar.2022.1015240/fulldehydroabietic acidliver cancerproteomicslipidomicsACSL3 |
| spellingShingle | Zhikai Xiahou Jun Han Jun Han Effects of dehydroabietic acid on nontarget lipidomics and proteomics of HepG2 Frontiers in Pharmacology dehydroabietic acid liver cancer proteomics lipidomics ACSL3 |
| title | Effects of dehydroabietic acid on nontarget lipidomics and proteomics of HepG2 |
| title_full | Effects of dehydroabietic acid on nontarget lipidomics and proteomics of HepG2 |
| title_fullStr | Effects of dehydroabietic acid on nontarget lipidomics and proteomics of HepG2 |
| title_full_unstemmed | Effects of dehydroabietic acid on nontarget lipidomics and proteomics of HepG2 |
| title_short | Effects of dehydroabietic acid on nontarget lipidomics and proteomics of HepG2 |
| title_sort | effects of dehydroabietic acid on nontarget lipidomics and proteomics of hepg2 |
| topic | dehydroabietic acid liver cancer proteomics lipidomics ACSL3 |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.1015240/full |
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