Evaluation of pathotype marker genes in Streptococcus suis isolated from human and clinically healthy swine in Thailand
Abstract Background Streptococcus suis is a zoonotic pathogen that causes substantial economic losses in the pig industry and contributes to human infections worldwide, especially in Southeast Asia. Recently, a multiplex polymerase chain reaction (PCR) process was developed to distinguish disease-as...
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| Format: | Article |
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BMC
2023-05-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-02888-9 |
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| author | Anusak Kerdsin Nichari Bamphensin Kulsatri Sittichottumrong Ratchadaporn Ungcharoen Parichart Boueroy Peechanika Chopjitt Rujirat Hatrongjit Marcelo Gottschalk Nuchsupha Sunthamala |
| author_facet | Anusak Kerdsin Nichari Bamphensin Kulsatri Sittichottumrong Ratchadaporn Ungcharoen Parichart Boueroy Peechanika Chopjitt Rujirat Hatrongjit Marcelo Gottschalk Nuchsupha Sunthamala |
| author_sort | Anusak Kerdsin |
| collection | DOAJ |
| description | Abstract Background Streptococcus suis is a zoonotic pathogen that causes substantial economic losses in the pig industry and contributes to human infections worldwide, especially in Southeast Asia. Recently, a multiplex polymerase chain reaction (PCR) process was developed to distinguish disease-associated and non-disease-associated pathotypes of S. suis European strains. Herein, we evaluated the ability of this multiplex PCR approach to distinguish pathotypes of S. suis in Thailand. Results This study was conducted on 278 human S. suis isolates and 173 clinically healthy pig S. suis isolates. PCR identified 99.3% of disease-associated strains in the human isolates and 11.6% of non-disease-associated strains in the clinically healthy pig isolates. Of the clinically healthy pig S. suis isolates, 71.1% were classified as disease-associated. We also detected undetermined pathotype forms in humans (0.7%) and pigs (17.3%). The PCR assay classified the disease-associated isolates into four types. Statistical analysis revealed that human S. suis clonal complex (CC) 1 isolates were significantly associated with the disease-associated type I, whereas CC104 and CC25 were significantly associated with the disease-associated type IV. Conclusion Multiplex PCR cannot differentiate non-disease-associated from disease-associated isolates in Thai clinically healthy pig S. suis strains, although the method works well for human S. suis strains. This assay should be applied to pig S. suis strains with caution. It is highly important that multiplex PCR be validated using more diverse S. suis strains from different geographic areas and origins of isolation. |
| first_indexed | 2024-03-13T10:16:43Z |
| format | Article |
| id | doaj.art-5ce71b19bc47425ab5f95319fb9e822f |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-13T10:16:43Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-5ce71b19bc47425ab5f95319fb9e822f2023-05-21T11:10:07ZengBMCBMC Microbiology1471-21802023-05-012311910.1186/s12866-023-02888-9Evaluation of pathotype marker genes in Streptococcus suis isolated from human and clinically healthy swine in ThailandAnusak Kerdsin0Nichari Bamphensin1Kulsatri Sittichottumrong2Ratchadaporn Ungcharoen3Parichart Boueroy4Peechanika Chopjitt5Rujirat Hatrongjit6Marcelo Gottschalk7Nuchsupha Sunthamala8Faculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province CampusFaculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province CampusFaculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province CampusFaculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province CampusFaculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province CampusFaculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province CampusFaculty of Science and Engineering, Kasetsart University Chalermphrakiat Sakon NakhonGroupe de recherche sur les maladies infectieuses en production animale (GREMIP), The Swine and Poultry Infectious Diseases Research Centre (CRIPA), Faculty of Veterinary Medicine, University of MontrealDepartment of Biology, Faculty of Science, Mahasarakham UniversityAbstract Background Streptococcus suis is a zoonotic pathogen that causes substantial economic losses in the pig industry and contributes to human infections worldwide, especially in Southeast Asia. Recently, a multiplex polymerase chain reaction (PCR) process was developed to distinguish disease-associated and non-disease-associated pathotypes of S. suis European strains. Herein, we evaluated the ability of this multiplex PCR approach to distinguish pathotypes of S. suis in Thailand. Results This study was conducted on 278 human S. suis isolates and 173 clinically healthy pig S. suis isolates. PCR identified 99.3% of disease-associated strains in the human isolates and 11.6% of non-disease-associated strains in the clinically healthy pig isolates. Of the clinically healthy pig S. suis isolates, 71.1% were classified as disease-associated. We also detected undetermined pathotype forms in humans (0.7%) and pigs (17.3%). The PCR assay classified the disease-associated isolates into four types. Statistical analysis revealed that human S. suis clonal complex (CC) 1 isolates were significantly associated with the disease-associated type I, whereas CC104 and CC25 were significantly associated with the disease-associated type IV. Conclusion Multiplex PCR cannot differentiate non-disease-associated from disease-associated isolates in Thai clinically healthy pig S. suis strains, although the method works well for human S. suis strains. This assay should be applied to pig S. suis strains with caution. It is highly important that multiplex PCR be validated using more diverse S. suis strains from different geographic areas and origins of isolation.https://doi.org/10.1186/s12866-023-02888-9Streptococcus suisSerotypeSequence typePathotypeMultiplex PCR |
| spellingShingle | Anusak Kerdsin Nichari Bamphensin Kulsatri Sittichottumrong Ratchadaporn Ungcharoen Parichart Boueroy Peechanika Chopjitt Rujirat Hatrongjit Marcelo Gottschalk Nuchsupha Sunthamala Evaluation of pathotype marker genes in Streptococcus suis isolated from human and clinically healthy swine in Thailand BMC Microbiology Streptococcus suis Serotype Sequence type Pathotype Multiplex PCR |
| title | Evaluation of pathotype marker genes in Streptococcus suis isolated from human and clinically healthy swine in Thailand |
| title_full | Evaluation of pathotype marker genes in Streptococcus suis isolated from human and clinically healthy swine in Thailand |
| title_fullStr | Evaluation of pathotype marker genes in Streptococcus suis isolated from human and clinically healthy swine in Thailand |
| title_full_unstemmed | Evaluation of pathotype marker genes in Streptococcus suis isolated from human and clinically healthy swine in Thailand |
| title_short | Evaluation of pathotype marker genes in Streptococcus suis isolated from human and clinically healthy swine in Thailand |
| title_sort | evaluation of pathotype marker genes in streptococcus suis isolated from human and clinically healthy swine in thailand |
| topic | Streptococcus suis Serotype Sequence type Pathotype Multiplex PCR |
| url | https://doi.org/10.1186/s12866-023-02888-9 |
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