The coupled photocycle of phenyl-p-benzoquinone and Light-Harvesting Complex II (LHCII) within the biohybrid system
Abstract The combination of trimeric form of the light-harvesting complex II (LHCII3), a porous graphite electrode (GE), and the application of phenyl-p-benzoquinone (PPBQ), the quinone derivative, allow the construction of a new type of biohybrid photoactive system. The Chl fluorescence decay and v...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-16892-y |
| _version_ | 1828430760925724672 |
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| author | Magdalena Łazicka Adriana Palińska-Saadi Paulina Piotrowska Bohdan Paterczyk Radosław Mazur Magdalena Maj-Żurawska Maciej Garstka |
| author_facet | Magdalena Łazicka Adriana Palińska-Saadi Paulina Piotrowska Bohdan Paterczyk Radosław Mazur Magdalena Maj-Żurawska Maciej Garstka |
| author_sort | Magdalena Łazicka |
| collection | DOAJ |
| description | Abstract The combination of trimeric form of the light-harvesting complex II (LHCII3), a porous graphite electrode (GE), and the application of phenyl-p-benzoquinone (PPBQ), the quinone derivative, allow the construction of a new type of biohybrid photoactive system. The Chl fluorescence decay and voltammetric analyzes revealed that PPBQ impacts LHCII3 proportionally to accessible quenching sites and that PPBQ forms redox complexes with Chl in both ground and excited states. As a result, photocurrent generation is directly dependent on PPBQ-induced quenching of Chl fluorescence. Since PPBQ also undergoes photoactivation, the action of GE-LHCII3-PPBQ depends on the mutual coupling of LHCII3 and PPBQ photocycles. The GE-LHCII3-PPBQ generates a photocurrent of up to 4.5 µA and exhibits considerable stability during operation. The three-dimensional arrangement of graphite scraps in GE builds an active electrode surface and stabilizes LHCII3 in its native form in low-density multilayers. The results indicate the future usability of such designed photoactive device. |
| first_indexed | 2024-12-10T17:53:29Z |
| format | Article |
| id | doaj.art-5ce9fa66912b4bcea9560378c19ca857 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-10T17:53:29Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5ce9fa66912b4bcea9560378c19ca8572022-12-22T01:39:01ZengNature PortfolioScientific Reports2045-23222022-07-0112111310.1038/s41598-022-16892-yThe coupled photocycle of phenyl-p-benzoquinone and Light-Harvesting Complex II (LHCII) within the biohybrid systemMagdalena Łazicka0Adriana Palińska-Saadi1Paulina Piotrowska2Bohdan Paterczyk3Radosław Mazur4Magdalena Maj-Żurawska5Maciej Garstka6Department of Metabolic Regulation, Faculty of Biology, Institute of Biochemistry, University of WarsawLaboratory of Basics of Analytical Chemistry, Faculty of Chemistry, University of WarsawDepartment of Metabolic Regulation, Faculty of Biology, Institute of Biochemistry, University of WarsawLaboratory of Electron and Confocal Microscopy, Faculty of Biology, University of WarsawDepartment of Metabolic Regulation, Faculty of Biology, Institute of Biochemistry, University of WarsawLaboratory of Basics of Analytical Chemistry, Faculty of Chemistry, University of WarsawDepartment of Metabolic Regulation, Faculty of Biology, Institute of Biochemistry, University of WarsawAbstract The combination of trimeric form of the light-harvesting complex II (LHCII3), a porous graphite electrode (GE), and the application of phenyl-p-benzoquinone (PPBQ), the quinone derivative, allow the construction of a new type of biohybrid photoactive system. The Chl fluorescence decay and voltammetric analyzes revealed that PPBQ impacts LHCII3 proportionally to accessible quenching sites and that PPBQ forms redox complexes with Chl in both ground and excited states. As a result, photocurrent generation is directly dependent on PPBQ-induced quenching of Chl fluorescence. Since PPBQ also undergoes photoactivation, the action of GE-LHCII3-PPBQ depends on the mutual coupling of LHCII3 and PPBQ photocycles. The GE-LHCII3-PPBQ generates a photocurrent of up to 4.5 µA and exhibits considerable stability during operation. The three-dimensional arrangement of graphite scraps in GE builds an active electrode surface and stabilizes LHCII3 in its native form in low-density multilayers. The results indicate the future usability of such designed photoactive device.https://doi.org/10.1038/s41598-022-16892-y |
| spellingShingle | Magdalena Łazicka Adriana Palińska-Saadi Paulina Piotrowska Bohdan Paterczyk Radosław Mazur Magdalena Maj-Żurawska Maciej Garstka The coupled photocycle of phenyl-p-benzoquinone and Light-Harvesting Complex II (LHCII) within the biohybrid system Scientific Reports |
| title | The coupled photocycle of phenyl-p-benzoquinone and Light-Harvesting Complex II (LHCII) within the biohybrid system |
| title_full | The coupled photocycle of phenyl-p-benzoquinone and Light-Harvesting Complex II (LHCII) within the biohybrid system |
| title_fullStr | The coupled photocycle of phenyl-p-benzoquinone and Light-Harvesting Complex II (LHCII) within the biohybrid system |
| title_full_unstemmed | The coupled photocycle of phenyl-p-benzoquinone and Light-Harvesting Complex II (LHCII) within the biohybrid system |
| title_short | The coupled photocycle of phenyl-p-benzoquinone and Light-Harvesting Complex II (LHCII) within the biohybrid system |
| title_sort | coupled photocycle of phenyl p benzoquinone and light harvesting complex ii lhcii within the biohybrid system |
| url | https://doi.org/10.1038/s41598-022-16892-y |
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