First molecular characterization of poxviruses in cattle, sheep, and goats in Botswana
Abstract Background Poxviruses within the Capripoxvirus, Orthopoxvirus, and Parapoxvirus genera can infect livestock, with the two former having zoonotic importance. In addition, they induce similar clinical symptoms in common host species, creating a challenge for diagnosis. Although endemic in the...
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| Format: | Article |
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BMC
2021-08-01
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| Series: | Virology Journal |
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| Online Access: | https://doi.org/10.1186/s12985-021-01634-9 |
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| author | Boitumelo Magret Modise Tirumala Bharani Kumar Settypalli Tebogo Kgotlele Dingrong Xue Kebonyemodisa Ntesang Kago Kumile Ivancho Naletoski John Frederick Nyange Carter Thanda Kenny Nametso Macheng Chandapiwa Marobela-Raborokgwe Gerrit Johannes Viljoen Giovanni Cattoli Charles Euloge Lamien |
| author_facet | Boitumelo Magret Modise Tirumala Bharani Kumar Settypalli Tebogo Kgotlele Dingrong Xue Kebonyemodisa Ntesang Kago Kumile Ivancho Naletoski John Frederick Nyange Carter Thanda Kenny Nametso Macheng Chandapiwa Marobela-Raborokgwe Gerrit Johannes Viljoen Giovanni Cattoli Charles Euloge Lamien |
| author_sort | Boitumelo Magret Modise |
| collection | DOAJ |
| description | Abstract Background Poxviruses within the Capripoxvirus, Orthopoxvirus, and Parapoxvirus genera can infect livestock, with the two former having zoonotic importance. In addition, they induce similar clinical symptoms in common host species, creating a challenge for diagnosis. Although endemic in the country, poxvirus infections of small ruminants and cattle have received little attention in Botswana, with no prior use of molecular tools to diagnose and characterize the pathogens. Methods A high-resolution melting (HRM) assay was used to detect and differentiate poxviruses in skin biopsy and skin scab samples from four cattle, one sheep, and one goat. Molecular characterization of capripoxviruses and parapoxviruses was undertaken by sequence analysis of RPO30 and GPCR genes. Results The HRM assay revealed lumpy skin disease virus (LSDV) in three cattle samples, pseudocowpox virus (PCPV) in one cattle sample, and orf virus (ORFV) in one goat and one sheep sample. The phylogenetic analyses, based on the RPO30 and GPCR multiple sequence alignments showed that the LSDV sequences of Botswana were similar to common LSDV field isolates encountered in Africa, Asia, and Europe. The Botswana PCPV presented unique features and clustered between camel and cattle PCPV isolates. The Botswana ORFV sequence isolated from goat differed from the ORFV sequence isolated from sheep. Conclusions This study is the first report on the genetic characterization of poxvirus diseases circulating in cattle, goats, and sheep in Botswana. It shows the importance of molecular methods to differentially diagnose poxvirus diseases of ruminants. |
| first_indexed | 2024-12-13T21:46:26Z |
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| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-13T21:46:26Z |
| publishDate | 2021-08-01 |
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| spelling | doaj.art-5cebf65bf2be4bb7a1c7fb2ee81898312022-12-21T23:30:24ZengBMCVirology Journal1743-422X2021-08-0118111110.1186/s12985-021-01634-9First molecular characterization of poxviruses in cattle, sheep, and goats in BotswanaBoitumelo Magret Modise0Tirumala Bharani Kumar Settypalli1Tebogo Kgotlele2Dingrong Xue3Kebonyemodisa Ntesang4Kago Kumile5Ivancho Naletoski6John Frederick Nyange7Carter Thanda8Kenny Nametso Macheng9Chandapiwa Marobela-Raborokgwe10Gerrit Johannes Viljoen11Giovanni Cattoli12Charles Euloge Lamien13Botswana National Veterinary LaboratoryAnimal Production and Health Section, Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna International CentreBotswana National Veterinary LaboratoryAnimal Production and Health Section, Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna International CentreBotswana National Veterinary LaboratoryBotswana National Veterinary LaboratoryAnimal Production and Health Section, Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna International CentreBotswana National Veterinary LaboratoryBotswana National Veterinary LaboratoryBotswana National Veterinary LaboratoryBotswana National Veterinary LaboratoryAnimal Production and Health Section, Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna International CentreAnimal Production and Health Section, Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna International CentreAnimal Production and Health Section, Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna International CentreAbstract Background Poxviruses within the Capripoxvirus, Orthopoxvirus, and Parapoxvirus genera can infect livestock, with the two former having zoonotic importance. In addition, they induce similar clinical symptoms in common host species, creating a challenge for diagnosis. Although endemic in the country, poxvirus infections of small ruminants and cattle have received little attention in Botswana, with no prior use of molecular tools to diagnose and characterize the pathogens. Methods A high-resolution melting (HRM) assay was used to detect and differentiate poxviruses in skin biopsy and skin scab samples from four cattle, one sheep, and one goat. Molecular characterization of capripoxviruses and parapoxviruses was undertaken by sequence analysis of RPO30 and GPCR genes. Results The HRM assay revealed lumpy skin disease virus (LSDV) in three cattle samples, pseudocowpox virus (PCPV) in one cattle sample, and orf virus (ORFV) in one goat and one sheep sample. The phylogenetic analyses, based on the RPO30 and GPCR multiple sequence alignments showed that the LSDV sequences of Botswana were similar to common LSDV field isolates encountered in Africa, Asia, and Europe. The Botswana PCPV presented unique features and clustered between camel and cattle PCPV isolates. The Botswana ORFV sequence isolated from goat differed from the ORFV sequence isolated from sheep. Conclusions This study is the first report on the genetic characterization of poxvirus diseases circulating in cattle, goats, and sheep in Botswana. It shows the importance of molecular methods to differentially diagnose poxvirus diseases of ruminants.https://doi.org/10.1186/s12985-021-01634-9Lumpy skin disease virusPseudocowpox virusOrf virusRPO30GPCRB2L gene |
| spellingShingle | Boitumelo Magret Modise Tirumala Bharani Kumar Settypalli Tebogo Kgotlele Dingrong Xue Kebonyemodisa Ntesang Kago Kumile Ivancho Naletoski John Frederick Nyange Carter Thanda Kenny Nametso Macheng Chandapiwa Marobela-Raborokgwe Gerrit Johannes Viljoen Giovanni Cattoli Charles Euloge Lamien First molecular characterization of poxviruses in cattle, sheep, and goats in Botswana Virology Journal Lumpy skin disease virus Pseudocowpox virus Orf virus RPO30 GPCR B2L gene |
| title | First molecular characterization of poxviruses in cattle, sheep, and goats in Botswana |
| title_full | First molecular characterization of poxviruses in cattle, sheep, and goats in Botswana |
| title_fullStr | First molecular characterization of poxviruses in cattle, sheep, and goats in Botswana |
| title_full_unstemmed | First molecular characterization of poxviruses in cattle, sheep, and goats in Botswana |
| title_short | First molecular characterization of poxviruses in cattle, sheep, and goats in Botswana |
| title_sort | first molecular characterization of poxviruses in cattle sheep and goats in botswana |
| topic | Lumpy skin disease virus Pseudocowpox virus Orf virus RPO30 GPCR B2L gene |
| url | https://doi.org/10.1186/s12985-021-01634-9 |
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