Sperm quality and DNA integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons
Objectives: The objective of this study was to assess sperm quality and deoxyribonucleic acid (DNA) integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) as compared to control subjects. Material and methods: The coke oven workers (N = 52) and administrative staff (N = 35...
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| Format: | Article |
| Language: | English |
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Nofer Institute of Occupational Medicine
2016-12-01
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| Series: | International Journal of Occupational Medicine and Environmental Health |
| Subjects: | |
| Online Access: | http://ijomeh.eu/Sperm-quality-and-DNA-integrity-of-coke-oven-workers-exposed-to-polycyclic-aromatic-hydrocarbons,60062,0,2.html |
| _version_ | 1811330970516717568 |
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| author | Hueiwang Anna Jeng Chih-Hong Pan Mu-Rong Chao Chien-Chih Chiu Guodong Zhou Chon-Kit Chou Wen-Yi Lin |
| author_facet | Hueiwang Anna Jeng Chih-Hong Pan Mu-Rong Chao Chien-Chih Chiu Guodong Zhou Chon-Kit Chou Wen-Yi Lin |
| author_sort | Hueiwang Anna Jeng |
| collection | DOAJ |
| description | Objectives: The objective of this study was to assess sperm quality and deoxyribonucleic acid (DNA) integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) as compared to control subjects. Material and methods: The coke oven workers (N = 52) and administrative staff (N = 35) of a steel plant served as the exposed and control groups, respectively. Exposure to PAHs was assessed by measuring 1-hydroxypyren. Analysis of sperm quality (concentration, motility, vitality, and morphology) was performed simultaneously with sperm DNA integrity analysis, including DNA fragmentation, denaturation, bulky DNA adducts, and 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxo-dGuo). A questionnaire was conducted to collect demographic and potential confounding data. Results: The coke oven workers had lower percentages of sperm motility, vitality and normal morphology than the control group, but the difference was not significant. For DNA integrity, the coke oven workers had significantly higher concentrations of bulky DNA adducts and 8-oxo-dGuo than the control subjects (p = 0.009 and p = 0.048, respectively). However, DNA fragmentation percentages did not significantly increase as compared to those in the subjects from the control group (p = 0.232). There was no correlation between sperm quality parameters and DNA integrity indicators. Conclusions: Occupational exposure of the coke oven workers to PAHs was associated with decreased sperm DNA integrity. Int J Occup Med Environ Health 2016;29(6):915–926 |
| first_indexed | 2024-04-13T16:10:50Z |
| format | Article |
| id | doaj.art-5cef240d0dad4fcd9d68629eff3604af |
| institution | Directory Open Access Journal |
| issn | 1232-1087 1896-494X |
| language | English |
| last_indexed | 2024-04-13T16:10:50Z |
| publishDate | 2016-12-01 |
| publisher | Nofer Institute of Occupational Medicine |
| record_format | Article |
| series | International Journal of Occupational Medicine and Environmental Health |
| spelling | doaj.art-5cef240d0dad4fcd9d68629eff3604af2022-12-22T02:40:14ZengNofer Institute of Occupational MedicineInternational Journal of Occupational Medicine and Environmental Health1232-10871896-494X2016-12-01296915926Sperm quality and DNA integrity of coke oven workers exposed to polycyclic aromatic hydrocarbonsHueiwang Anna JengChih-Hong PanMu-Rong ChaoChien-Chih ChiuGuodong ZhouChon-Kit ChouWen-Yi LinObjectives: The objective of this study was to assess sperm quality and deoxyribonucleic acid (DNA) integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) as compared to control subjects. Material and methods: The coke oven workers (N = 52) and administrative staff (N = 35) of a steel plant served as the exposed and control groups, respectively. Exposure to PAHs was assessed by measuring 1-hydroxypyren. Analysis of sperm quality (concentration, motility, vitality, and morphology) was performed simultaneously with sperm DNA integrity analysis, including DNA fragmentation, denaturation, bulky DNA adducts, and 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxo-dGuo). A questionnaire was conducted to collect demographic and potential confounding data. Results: The coke oven workers had lower percentages of sperm motility, vitality and normal morphology than the control group, but the difference was not significant. For DNA integrity, the coke oven workers had significantly higher concentrations of bulky DNA adducts and 8-oxo-dGuo than the control subjects (p = 0.009 and p = 0.048, respectively). However, DNA fragmentation percentages did not significantly increase as compared to those in the subjects from the control group (p = 0.232). There was no correlation between sperm quality parameters and DNA integrity indicators. Conclusions: Occupational exposure of the coke oven workers to PAHs was associated with decreased sperm DNA integrity. Int J Occup Med Environ Health 2016;29(6):915–926http://ijomeh.eu/Sperm-quality-and-DNA-integrity-of-coke-oven-workers-exposed-to-polycyclic-aromatic-hydrocarbons,60062,0,2.htmlDNA damagesemen qualityspermDNA integrityDNA fragmentationbulky DNA adducts |
| spellingShingle | Hueiwang Anna Jeng Chih-Hong Pan Mu-Rong Chao Chien-Chih Chiu Guodong Zhou Chon-Kit Chou Wen-Yi Lin Sperm quality and DNA integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons International Journal of Occupational Medicine and Environmental Health DNA damage semen quality sperm DNA integrity DNA fragmentation bulky DNA adducts |
| title | Sperm quality and DNA integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons |
| title_full | Sperm quality and DNA integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons |
| title_fullStr | Sperm quality and DNA integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons |
| title_full_unstemmed | Sperm quality and DNA integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons |
| title_short | Sperm quality and DNA integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons |
| title_sort | sperm quality and dna integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons |
| topic | DNA damage semen quality sperm DNA integrity DNA fragmentation bulky DNA adducts |
| url | http://ijomeh.eu/Sperm-quality-and-DNA-integrity-of-coke-oven-workers-exposed-to-polycyclic-aromatic-hydrocarbons,60062,0,2.html |
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